P57530 (EX5A_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Exodeoxyribonuclease V alpha chain
EC=3.1.11.5

Gene names

Name:	recD
Ordered Locus Names:	BU455

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exhibits several catalytic activities, including ATP-dependent exonuclease, ATP-stimulated endonuclease, ATP-dependent unwinding and DNA-dependent ATPase activities. Strand cleavage occurs 5' to 3' during the unwinding of duplex DNA at CHI sequences, which locally stimulate recombination By similarity.
Catalytic activity	Exonucleolytic cleavage (in the presence of ATP) in either 5'- to 3'- or 3'- to 5'-direction to yield 5'-phosphooligonucleotides.
Subunit structure	Consists of three subunits; RecB, RecC and RecD By similarity.
Sequence similarities	Belongs to the RecD family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Ligand	ATP-binding Nucleotide-binding
Molecular function	Endonuclease Exonuclease Helicase Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	exodeoxyribonuclease V complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW exodeoxyribonuclease V activity Inferred from electronic annotation. Source: EC helicase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Exodeoxyribonuclease V alpha chain		PRO_0000087112
Regions
	Nucleotide binding	171 – 178	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P57530 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 340FFAE9BB436059	FASTA	602	69,495
	10 20 30 40 50 60 MLILLKKAVK LKIIRPIDFY FSQFIAQKNN IVMLVAACVS YESSRGYISL PIKYFEKHYF 70 80 90 100 110 120 FSSSNEVFIK KILTLLEKKI NWPVELLKHA SIGNGGTSTP LVLHKKKIYL YKMWKSESNI 130 140 150 160 170 180 FNYLYTKNKK NKINQKKCSK ILENLFPQKN MSFQKIAVAL TLINNITFII GGPGTGKTTT 190 200 210 220 230 240 ILKIIIALIK SSKKSIKIQL SAPTGKATTH LNEILKNNIF DLYFSEKEKC SLPSTATTIH 250 260 270 280 290 300 QLLGIQKISQ KSFFNKSNCL DLDVLIIDEI SMVDILMMEK ILSSISKNTK LIFIGDHNQL 310 320 330 340 350 360 GPIESGSILR KICYYANDGY SFKSMISIEK LTQYKLCKKI NKKTTNFISD NICVLNKNYR 370 380 390 400 410 420 FNKNSGIYTL SNAIFKKKTR IIESLFDNSI KNIFFYETNS SEQYKKMIKN ICLNYEDFWE 430 440 450 460 470 480 KIYKKATMKE IIESFQNYQV LCILHDGLFG VNIINKKLEE NMYKKNIIKY FYIDGEEWYI 490 500 510 520 530 540 GKPIMIINNN RALNVSNGNI GITNINKNGI LQVSFLKENN TINNIPVKIL RNYKTAWAIT 550 560 570 580 590 600 VHKSQGSEFM NTALILPNFN SHILNKDTLY TGITRSRKIL SIFSDKKIFL NTIFKNTNKI LF

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13153.1.
RefSeq	NP_240267.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57530.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13153; BAB13153; BAB13153.
GeneID	1109803.
KEGG	buc:BU455.
PATRIC	21244452. VBIBucAph127364_0465.
Phylogenomic databases
eggNOG	COG0507.
HOGENOM	HOG000258341.
KO	K03581.
OMA	LFNGERG.
ProtClustDB	CLSK552876.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR006344. ExoDNase_V_RecD_asu. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01447. recD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

EX5A_BUCAI

Accession

Primary (citable) accession number: P57530

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program