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Protein

Fructose-bisphosphate aldolase class 2

Gene

fbaA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei61 – 611Glyceraldehyde 3-phosphateBy similarity
Active sitei109 – 1091Proton donorBy similarity
Metal bindingi110 – 1101Zinc 1; catalyticBy similarity
Metal bindingi144 – 1441Zinc 2By similarity
Metal bindingi174 – 1741Zinc 2By similarity
Metal bindingi226 – 2261Zinc 1; catalyticBy similarity
Binding sitei227 – 2271Dihydroxyacetone phosphate; via amide nitrogenBy similarity
Metal bindingi264 – 2641Zinc 1; catalyticBy similarity

GO - Molecular functioni

  1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC
  2. zinc ion binding Source: InterPro

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-451-MONOMER.
UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase class 2 (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Fructose-bisphosphate aldolase class II
Gene namesi
Name:fbaA
Synonyms:fba
Ordered Locus Names:BU451
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 358358Fructose-bisphosphate aldolase class 2PRO_0000178708Add
BLAST

Proteomic databases

PRIDEiP57526.

Structurei

3D structure databases

ProteinModelPortaliP57526.
SMRiP57526. Positions 3-358.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni265 – 2673Dihydroxyacetone phosphate bindingBy similarity
Regioni286 – 2894Dihydroxyacetone phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG69GZPB.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILNVIRPG VMNGDEARIV FELAKKKQFA IPAVNCIGTD SINAVLETAA
60 70 80 90 100
RVKSPIIIQF SHGGASFIAG YKKKLSENQE QAIQGAVSGA QHVHLMAKHY
110 120 130 140 150
EIPVILHTDH CPKETLSWID GLLEVGQNYY FNNNRPLFTS HMIDLSKESL
160 170 180 190 200
EENISTCKKY FKRIKNINMM LEIELGCTGG EEDGIDNTKI DKKLLYTQPQ
210 220 230 240 250
DVNYAYEELN TISKNFSIAA SFGNIHGVYQ PGNIDLRPII LKNSQEFVSS
260 270 280 290 300
KHNLEKNPLN LVFHGGSGSN LKEIKESIQY GVVKMNIDTD IQWAAWKGVL
310 320 330 340 350
DFYKQNKEFL QHQLGNTTNK HKPNKKYYDP RTWIRKSQES ISIRLEQSFK

ELNSFNIL
Length:358
Mass (Da):40,663
Last modified:December 1, 2000 - v1
Checksum:iAB7A47FBF80D2FC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13149.1.
RefSeqiNP_240263.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13149; BAB13149; BAB13149.
GeneIDi1109799.
KEGGibuc:BU451.
PATRICi21244444. VBIBucAph127364_0461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13149.1.
RefSeqiNP_240263.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57526.
SMRiP57526. Positions 3-358.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP57526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13149; BAB13149; BAB13149.
GeneIDi1109799.
KEGGibuc:BU451.
PATRICi21244444. VBIBucAph127364_0461.

Phylogenomic databases

eggNOGiCOG0191.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.
OrthoDBiEOG69GZPB.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.
BioCyciBAPH107806:GBZJ-451-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR006411. Fruct_bisP_bact.
IPR000771. Ketose_bisP_aldolase_II.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiALF_BUCAI
AccessioniPrimary (citable) accession number: P57526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.