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Protein

Fructose-bisphosphate aldolase class 2

Gene

fbaA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase class 2 (fbaA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei61Glyceraldehyde 3-phosphateBy similarity1
Active sitei109Proton donorBy similarity1
Metal bindingi110Zinc 1; catalyticBy similarity1
Metal bindingi144Zinc 2By similarity1
Metal bindingi174Zinc 2By similarity1
Metal bindingi226Zinc 1; catalyticBy similarity1
Binding sitei227Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi264Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase class 2 (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Fructose-bisphosphate aldolase class II
Gene namesi
Name:fbaA
Synonyms:fba
Ordered Locus Names:BU451
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001787081 – 358Fructose-bisphosphate aldolase class 2Add BLAST358

Proteomic databases

PRIDEiP57526.

Interactioni

Protein-protein interaction databases

STRINGi107806.BU451.

Structurei

3D structure databases

ProteinModelPortaliP57526.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni265 – 267Dihydroxyacetone phosphate bindingBy similarity3
Regioni286 – 289Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105D2N. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57526-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNILNVIRPG VMNGDEARIV FELAKKKQFA IPAVNCIGTD SINAVLETAA
60 70 80 90 100
RVKSPIIIQF SHGGASFIAG YKKKLSENQE QAIQGAVSGA QHVHLMAKHY
110 120 130 140 150
EIPVILHTDH CPKETLSWID GLLEVGQNYY FNNNRPLFTS HMIDLSKESL
160 170 180 190 200
EENISTCKKY FKRIKNINMM LEIELGCTGG EEDGIDNTKI DKKLLYTQPQ
210 220 230 240 250
DVNYAYEELN TISKNFSIAA SFGNIHGVYQ PGNIDLRPII LKNSQEFVSS
260 270 280 290 300
KHNLEKNPLN LVFHGGSGSN LKEIKESIQY GVVKMNIDTD IQWAAWKGVL
310 320 330 340 350
DFYKQNKEFL QHQLGNTTNK HKPNKKYYDP RTWIRKSQES ISIRLEQSFK

ELNSFNIL
Length:358
Mass (Da):40,663
Last modified:December 1, 2000 - v1
Checksum:iAB7A47FBF80D2FC2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13149.1.
RefSeqiNP_240263.1. NC_002528.1.
WP_009874405.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13149; BAB13149; BAB13149.
GeneIDi1109799.
KEGGibuc:BU451.
PATRICi21244444. VBIBucAph127364_0461.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13149.1.
RefSeqiNP_240263.1. NC_002528.1.
WP_009874405.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57526.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU451.

Proteomic databases

PRIDEiP57526.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13149; BAB13149; BAB13149.
GeneIDi1109799.
KEGGibuc:BU451.
PATRICi21244444. VBIBucAph127364_0461.

Phylogenomic databases

eggNOGiENOG4105D2N. Bacteria.
COG0191. LUCA.
HOGENOMiHOG000227794.
KOiK01624.
OMAiRMSKMGM.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Family and domain databases

CDDicd00946. FBP_aldolase_IIA. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR006411. Fruct_bisP_bact.
[Graphical view]
PfamiPF01116. F_bP_aldolase. 1 hit.
[Graphical view]
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01520. FruBisAldo_II_A. 1 hit.
PROSITEiPS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALF_BUCAI
AccessioniPrimary (citable) accession number: P57526
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.