Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P57519 (SYL_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BU444
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length859 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 859859Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151987

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P57519 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 43DBB88CD4020975

FASTA859102,170
        10         20         30         40         50         60 
MEKEYSPKKI ENYVQEFWKK NKTFEVKEDP KKEKYYCLPM LPYPSGKLHM GHVRNYTISD 

        70         80         90        100        110        120 
VISRYQRMLG KNVLQPMGWD AFGLPAEEAA IRNNTDPFSW TQKNIKYMKK QLQSLGFSYD 

       130        140        150        160        170        180 
WSREITTCHP EYYHWEQWFF TKLYEKKLVY KKNSLVNWCS YDKTVLANEQ VIDGCCWRCQ 

       190        200        210        220        230        240 
NKIRIKQIPQ WFIKIRNYAE SLYQDLKKLT HWPENVKNMQ RNWIGRIKGF EITLNVFNTC 

       250        260        270        280        290        300 
QKLKVFTQRL DLLMGVTYIS ISSCHKLSIN LSKKNELIKK FIKKYRYISQ EEQYKVKYTG 

       310        320        330        340        350        360 
INTNLFVVHP ITKKTIPIWI SNATHIEYGT NAVLSIPGHN ENDWNFAVKN NLKIKYVIFN 

       370        380        390        400        410        420 
PDHQEPKLYT SFLDIKGTLF NSQEFNGLNL KDGTEKIKKI LYKKKILKEK INYKLQDWCI 

       430        440        450        460        470        480 
SRQRYWGTPI PMAKFKNGKM IPIPENQLPV VLPKIRKNTN LLQQAINFNS KWAEIFIHGK 

       490        500        510        520        530        540 
HAIREIDTFD TFMESSWYYA RYTCPNFNTG MIDSIASKYW LPVDQYIGGI EHAIMHLMYF 

       550        560        570        580        590        600 
RFYHKLLRDF KLVDFDEPVK NLLCQGMVLS EAFYKIDSNS QRKWFNSSSV LIKRNTKGEI 

       610        620        630        640        650        660 
IESHTQKGEK LIYAGMIKMS KSKNNGIEPE LIIQRYGADT IRLFIMFSAP VESDLEWKES 

       670        680        690        700        710        720 
GLKGIYRFLK KLWMLIFNYI DIKNTHKKIN FDFLNHQQSE LRYQLHKTIA KVSDDIGRRQ 

       730        740        750        760        770        780 
TFNTAISEIM KLVNQLSKAP IKEEQDKSIM RESLICIIKM LYPFTPHFCF FVWNYFNNHS 

       790        800        810        820        830        840 
SIDNEKWPIF QKDILSKKYS TIVAQINGKK RCATKISDSL TKEEIFLYIQ NQPIIKKYLE 

       850 
DVDIKKIIYI PKKIINFVT 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13142.1.
RefSeqNP_240256.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57519.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13142; BAB13142; BAB13142.
GeneID1109792.
KEGGbuc:BU444.
PATRIC21244430. VBIBucAph127364_0454.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAHISHELW.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-444-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BUCAI
AccessionPrimary (citable) accession number: P57519
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries