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P57519

- SYL_BUCAI

UniProt

P57519 - SYL_BUCAI

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei621 – 6211ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-444-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:BU444
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 859859Leucine--tRNA ligasePRO_0000151987Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP57519.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi42 – 5211"HIGH" regionAdd
    BLAST
    Motifi618 – 6225"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG63Z74X.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57519-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKEYSPKKI ENYVQEFWKK NKTFEVKEDP KKEKYYCLPM LPYPSGKLHM    50
    GHVRNYTISD VISRYQRMLG KNVLQPMGWD AFGLPAEEAA IRNNTDPFSW 100
    TQKNIKYMKK QLQSLGFSYD WSREITTCHP EYYHWEQWFF TKLYEKKLVY 150
    KKNSLVNWCS YDKTVLANEQ VIDGCCWRCQ NKIRIKQIPQ WFIKIRNYAE 200
    SLYQDLKKLT HWPENVKNMQ RNWIGRIKGF EITLNVFNTC QKLKVFTQRL 250
    DLLMGVTYIS ISSCHKLSIN LSKKNELIKK FIKKYRYISQ EEQYKVKYTG 300
    INTNLFVVHP ITKKTIPIWI SNATHIEYGT NAVLSIPGHN ENDWNFAVKN 350
    NLKIKYVIFN PDHQEPKLYT SFLDIKGTLF NSQEFNGLNL KDGTEKIKKI 400
    LYKKKILKEK INYKLQDWCI SRQRYWGTPI PMAKFKNGKM IPIPENQLPV 450
    VLPKIRKNTN LLQQAINFNS KWAEIFIHGK HAIREIDTFD TFMESSWYYA 500
    RYTCPNFNTG MIDSIASKYW LPVDQYIGGI EHAIMHLMYF RFYHKLLRDF 550
    KLVDFDEPVK NLLCQGMVLS EAFYKIDSNS QRKWFNSSSV LIKRNTKGEI 600
    IESHTQKGEK LIYAGMIKMS KSKNNGIEPE LIIQRYGADT IRLFIMFSAP 650
    VESDLEWKES GLKGIYRFLK KLWMLIFNYI DIKNTHKKIN FDFLNHQQSE 700
    LRYQLHKTIA KVSDDIGRRQ TFNTAISEIM KLVNQLSKAP IKEEQDKSIM 750
    RESLICIIKM LYPFTPHFCF FVWNYFNNHS SIDNEKWPIF QKDILSKKYS 800
    TIVAQINGKK RCATKISDSL TKEEIFLYIQ NQPIIKKYLE DVDIKKIIYI 850
    PKKIINFVT 859
    Length:859
    Mass (Da):102,170
    Last modified:December 1, 2000 - v1
    Checksum:i43DBB88CD4020975
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13142.1.
    RefSeqiNP_240256.1. NC_002528.1.
    WP_010896120.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13142; BAB13142; BAB13142.
    GeneIDi1109792.
    KEGGibuc:BU444.
    PATRICi21244430. VBIBucAph127364_0454.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13142.1 .
    RefSeqi NP_240256.1. NC_002528.1.
    WP_010896120.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57519.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13142 ; BAB13142 ; BAB13142 .
    GeneIDi 1109792.
    KEGGi buc:BU444.
    PATRICi 21244430. VBIBucAph127364_0454.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG63Z74X.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-444-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiSYL_BUCAI
    AccessioniPrimary (citable) accession number: P57519
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3