Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P57516 (MIAB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

EC=2.8.4.3
Alternative name(s):
(Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
tRNA-i(6)A37 methylthiotransferase
Gene names
Name:miaB
Ordered Locus Names:BU441
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length439 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine By similarity. HAMAP-Rule MF_01864

Catalytic activity

N(6)-dimethylallyladenine37 in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine37 in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine. HAMAP-Rule MF_01864

Cofactor

Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01864

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01864.

Sequence similarities

Belongs to the methylthiotransferase family. MiaB subfamily.

Contains 1 MTTase N-terminal domain.

Contains 1 TRAM domain.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA modification

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

transferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 439439tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase HAMAP-Rule MF_01864
PRO_0000141730

Regions

Domain2 – 119118MTTase N-terminal
Domain377 – 43963TRAM

Sites

Metal binding111Iron-sulfur (4Fe-4S) By similarity
Metal binding481Iron-sulfur (4Fe-4S) By similarity
Metal binding821Iron-sulfur (4Fe-4S) By similarity
Metal binding1561Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1601Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding1631Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
P57516 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 287361B42483D610

FASTA43950,465
        10         20         30         40         50         60 
MKYIYIKTWG CQMNEYDSSM IITLLEKNNQ YSLTKSAENA DILILNTCSI REKAQEKVFH 

        70         80         90        100        110        120 
QLGRWKKIKN NNPKVIIAVG GCVATQEGKE IFKRANYVDI IFGTQTLHRL PKMIDEVEKK 

       130        140        150        160        170        180 
RKLSIDISFP KLEKFKYFLA PKKKGYTADI SIMEGCNKYC SFCVVPYTRG NEISRPCDDV 

       190        200        210        220        230        240 
LFEISLLAKQ GIKEINLLGQ NVNAYQGPTF NGKVCYFSEL IRLVAEIDGI ERIRFTTSNP 

       250        260        270        280        290        300 
LEFTDDIIEV YKDTPKLVSF LHLPVQSGSN KILNLMKRSY TTEDYTSIIK KLTIARPDIQ 

       310        320        330        340        350        360 
ISSDFIVGFP GESEIDFEKT IEFIKNINFD MSFSFIYSAR PGTPASNMND DLDLKEKKRR 

       370        380        390        400        410        420 
LYILQERINI QTMLWSRKMF GSIQSVLVEG VSDKNIMDLY GRTENNRVVT FQGSSKMIGQ 

       430 
FVNVKIKKVH THSLKGELF 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13139.1.
RefSeqNP_240253.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57516.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13139; BAB13139; BAB13139.
GeneID1109789.
KEGGbuc:BU441.
PATRIC21244422. VBIBucAph127364_0450.

Phylogenomic databases

eggNOGCOG0621.
HOGENOMHOG000224767.
KOK06168.
OMAIEVYKDT.
OrthoDBEOG6P5ZD8.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-441-MONOMER.

Family and domain databases

Gene3D3.80.30.20. 1 hit.
HAMAPMF_01864. tRNA_metthiotr_MiaB.
InterProIPR006638. Elp3/MiaB/NifB.
IPR023970. MeThioTfrase/rSAM.
IPR005839. Methylthiotransferase.
IPR020612. Methylthiotransferase_CS.
IPR013848. Methylthiotransferase_N.
IPR006463. MiaB_methiolase.
IPR007197. rSAM.
IPR023404. rSAM_horseshoe.
IPR002792. TRAM_dom.
[Graphical view]
PANTHERPTHR11918. PTHR11918. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
PF01938. TRAM. 1 hit.
PF00919. UPF0004. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00089. TIGR00089. 1 hit.
PROSITEPS51449. MTTASE_N. 1 hit.
PS01278. MTTASE_RADICAL. 1 hit.
PS50926. TRAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMIAB_BUCAI
AccessionPrimary (citable) accession number: P57516
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names