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P57516

- MIAB_BUCAI

UniProt

P57516 - MIAB_BUCAI

Protein

tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase

Gene

miaB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i6A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms2i6A) at position 37 in tRNAs that read codons beginning with uridine.UniRule annotation

    Catalytic activityi

    N(6)-dimethylallyladenine(37) in tRNA + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = 2-methylthio-N(6)-dimethylallyladenine(37) in tRNA + S-adenosyl-L-homocysteine + (sulfur carrier) + L-methionine + 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi11 – 111Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi48 – 481Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi82 – 821Iron-sulfur (4Fe-4S)UniRule annotation
    Metal bindingi156 – 1561Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi160 – 1601Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi163 – 1631Iron-sulfur (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. iron ion binding Source: UniProtKB-HAMAP
    3. transferase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. tRNA modification Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-441-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA-2-methylthio-N(6)-dimethylallyladenosine synthaseUniRule annotation (EC:2.8.4.3UniRule annotation)
    Alternative name(s):
    (Dimethylallyl)adenosine tRNA methylthiotransferase MiaBUniRule annotation
    tRNA-i(6)A37 methylthiotransferaseUniRule annotation
    Gene namesi
    Name:miaBUniRule annotation
    Ordered Locus Names:BU441
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 439439tRNA-2-methylthio-N(6)-dimethylallyladenosine synthasePRO_0000141730Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57516.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 119118MTTase N-terminalUniRule annotationAdd
    BLAST
    Domaini377 – 43963TRAMUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the methylthiotransferase family. MiaB subfamily.UniRule annotation
    Contains 1 MTTase N-terminal domain.UniRule annotation
    Contains 1 TRAM domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0621.
    HOGENOMiHOG000224767.
    KOiK06168.
    OMAiIEVYKDT.
    OrthoDBiEOG6P5ZD8.

    Family and domain databases

    Gene3Di3.80.30.20. 1 hit.
    HAMAPiMF_01864. tRNA_metthiotr_MiaB.
    InterProiIPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view]
    PANTHERiPTHR11918. PTHR11918. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view]
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00089. TIGR00089. 1 hit.
    PROSITEiPS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57516-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKYIYIKTWG CQMNEYDSSM IITLLEKNNQ YSLTKSAENA DILILNTCSI    50
    REKAQEKVFH QLGRWKKIKN NNPKVIIAVG GCVATQEGKE IFKRANYVDI 100
    IFGTQTLHRL PKMIDEVEKK RKLSIDISFP KLEKFKYFLA PKKKGYTADI 150
    SIMEGCNKYC SFCVVPYTRG NEISRPCDDV LFEISLLAKQ GIKEINLLGQ 200
    NVNAYQGPTF NGKVCYFSEL IRLVAEIDGI ERIRFTTSNP LEFTDDIIEV 250
    YKDTPKLVSF LHLPVQSGSN KILNLMKRSY TTEDYTSIIK KLTIARPDIQ 300
    ISSDFIVGFP GESEIDFEKT IEFIKNINFD MSFSFIYSAR PGTPASNMND 350
    DLDLKEKKRR LYILQERINI QTMLWSRKMF GSIQSVLVEG VSDKNIMDLY 400
    GRTENNRVVT FQGSSKMIGQ FVNVKIKKVH THSLKGELF 439
    Length:439
    Mass (Da):50,465
    Last modified:December 1, 2000 - v1
    Checksum:i287361B42483D610
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13139.1.
    RefSeqiNP_240253.1. NC_002528.1.
    WP_010896118.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13139; BAB13139; BAB13139.
    GeneIDi1109789.
    KEGGibuc:BU441.
    PATRICi21244422. VBIBucAph127364_0450.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13139.1 .
    RefSeqi NP_240253.1. NC_002528.1.
    WP_010896118.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57516.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13139 ; BAB13139 ; BAB13139 .
    GeneIDi 1109789.
    KEGGi buc:BU441.
    PATRICi 21244422. VBIBucAph127364_0450.

    Phylogenomic databases

    eggNOGi COG0621.
    HOGENOMi HOG000224767.
    KOi K06168.
    OMAi IEVYKDT.
    OrthoDBi EOG6P5ZD8.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-441-MONOMER.

    Family and domain databases

    Gene3Di 3.80.30.20. 1 hit.
    HAMAPi MF_01864. tRNA_metthiotr_MiaB.
    InterProi IPR006638. Elp3/MiaB/NifB.
    IPR023970. MeThioTfrase/rSAM.
    IPR005839. Methylthiotransferase.
    IPR020612. Methylthiotransferase_CS.
    IPR013848. Methylthiotransferase_N.
    IPR006463. MiaB_methiolase.
    IPR007197. rSAM.
    IPR023404. rSAM_horseshoe.
    IPR002792. TRAM_dom.
    [Graphical view ]
    PANTHERi PTHR11918. PTHR11918. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    PF01938. TRAM. 1 hit.
    PF00919. UPF0004. 1 hit.
    [Graphical view ]
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00089. TIGR00089. 1 hit.
    PROSITEi PS51449. MTTASE_N. 1 hit.
    PS01278. MTTASE_RADICAL. 1 hit.
    PS50926. TRAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiMIAB_BUCAI
    AccessioniPrimary (citable) accession number: P57516
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3