P57503 (CYSJ_BUCAI) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 72.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Sulfite reductase [NADPH] flavoprotein alpha-component Short name=SiR-FP EC=1.8.1.2 | ||||
| Gene names |
| ||||
| Organism | Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) | ||||
| Taxonomic identifier | 107806 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera |
Protein attributes
| Sequence length | 601 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity. HAMAP MF_01541 |
| Catalytic activity | H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01541 |
| Cofactor | Binds 1 FAD per subunit By similarity. HAMAP MF_01541 Binds 1 FMN per subunit By similarity. HAMAP MF_01541 |
| Pathway | Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01541 |
| Subunit structure | Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. |
| Sequence similarities | Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Amino-acid biosynthesis Cysteine biosynthesis Electron transport Transport |
| Ligand | FAD FMN Flavoprotein NADP |
| Molecular function | Oxidoreductase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chainInferred from electronic annotation. Source: UniProtKB-KW sulfate assimilationInferred from electronic annotation. Source: InterPro transportInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | FMN binding Inferred from electronic annotation. Source: InterPro flavin adenine dinucleotide bindingInferred from electronic annotation. Source: InterPro iron ion bindingInferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 601 | 601 | Sulfite reductase [NADPH] flavoprotein alpha-component HAMAP MF_01541 | PRO_0000199920 | |||||
Regions | |||||||||
| Domain | 65 – 203 | 139 | Flavodoxin-like | ||||||
| Domain | 236 – 450 | 215 | FAD-binding FR-type | ||||||
| Nucleotide binding | 71 – 75 | 5 | FMN By similarity | ||||||
| Nucleotide binding | 118 – 123 | 6 | FMN By similarity | ||||||
| Nucleotide binding | 151 – 182 | 32 | FMN By similarity | ||||||
| Nucleotide binding | 388 – 391 | 4 | FAD By similarity | ||||||
| Nucleotide binding | 422 – 424 | 3 | FAD By similarity | ||||||
| Nucleotide binding | 521 – 529 | 9 | NADP By similarity | ||||||
Sites | |||||||||
| Binding site | 491 | 1 | NADP By similarity | ||||||
Sequences
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References
| [1] | "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | BA000003 Genomic DNA. Translation: BAB13126.1. |
| RefSeq | NP_240240.1. NC_002528.1. |
3D structure databases | |
| ProteinModelPortal | P57503. |
| SMR | P57503. Positions 229-601. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblBacteria | EBBUCT00000003311; EBBUCP00000003087; EBBUCG00000003311. |
| GeneID | 1109776. |
| GenomeReviews | Gene locus BU428 in contig BA000003_GR. |
| KEGG | buc:BU428. |
| PATRIC | 21244396. VBIBucAph127364_0437. |
Phylogenomic databases | |
| GeneTree | EBGT00050000008144. |
| HOGENOM | HBG736048. |
| OMA | ESADEYL. |
| PhylomeDB | P57503. |
| ProtClustDB | CLSK316168. |
Enzyme and pathway databases | |
| BioCyc | BSP107806:BU428-MONOMER. |
Family and domain databases | |
| HAMAP | MF_01541. CysJ. [Tree] |
| InterPro | IPR010199. CysJ. IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR023173. NADPH_Cyt_P450_Rdtase_dom3. IPR001433. OxRdtase_FAD/NAD-bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view] |
| Gene3D | G3DSA:1.20.990.10. NADPH_Cyt_P450_Rdtase_dom3. 1 hit. |
| KO | K00380. |
| Pfam | PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF000207. SiR-FP_CysJ. 1 hit. |
| PRINTS | PR00369. FLAVODOXIN. PR00371. FPNCR. |
| SUPFAM | SSF63380. Riboflavin_synthase_like_b-brl. 1 hit. |
| TIGRFAMs | TIGR01931. CysJ. 1 hit. |
| PROSITE | PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | CYSJ_BUCAI | ||||||||
| Accession | Primary (citable) accession number: P57503 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Buchnera aphidicola (subsp. Acyrthosiphon pisum) Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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