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P57503

- CYSJ_BUCAI

UniProt

P57503 - CYSJ_BUCAI

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Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene
cysJ, BU428
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Binds 1 FAD per subunit By similarity.UniRule annotation
Binds 1 FMN per subunit By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei491 – 4911NADP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 755FMN By similarity
Nucleotide bindingi118 – 1236FMN By similarity
Nucleotide bindingi151 – 18232FMN By similarityAdd
BLAST
Nucleotide bindingi388 – 3914FAD By similarity
Nucleotide bindingi422 – 4243FAD By similarity
Nucleotide bindingi521 – 5299NADP By similarity

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-428-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-component (EC:1.8.1.2)
Short name:
SiR-FP
Gene namesi
Name:cysJ
Ordered Locus Names:BU428
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotationPRO_0000199920Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57503.
SMRiP57503. Positions 229-601.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 203139Flavodoxin-likeAdd
BLAST
Domaini236 – 450215FAD-binding FR-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282025.
KOiK00380.
OMAiHEFLQSK.
OrthoDBiEOG6CVV7G.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57503-1 [UniParc]FASTAAdd to Basket

« Hide

MKNQNKFDPV FPLSSEQLNN LKELERTCTN IQSAWLSGYF WKIANQTSNI    50
TSFQTNESEK NDPVITIISA SQTGNAKLLS KRLYEYFNKN NKISRLIDAM 100
DYKFKKIKDE KILILIISTQ GEGEPPEEAL SFYKFIMSKN APNLNNLYYS 150
VFGLGDTSYN LFCQAGKDFD KRFKELGGNS LLDRFDADIE YEDNYNKWSQ 200
DLLQSINSKE KIYKSSVSYI DQENTLILSK NHYTKKNPAE GIILTNQKIT 250
GRNSKKDVHH IEIDISNLNI KYSPGDALGV WYKNDSNLVK NILELLSINI 300
SETITIKNDV ITIFDALQNH FELTNNTKNI IKSYANFSKN KFLKDIISND 350
SDLENYTINT PLIKMIHDHP LKLSSQQLIG LLRPLTPRLY SISSSQEEID 400
NEIHITVGVV KKLISGHVYL GGASGYLSQS LKSDDIIKIF IQTNDNFRLP 450
INKNTPIIMI SSGTGIAPFR AFMQQRDNDN ADGKNWLFFG NPNFTEDFLY 500
QVEWQKYIKK GLITNMNLAW SQDQKNKIYV QDRIRENSQE IWSWIEEGAQ 550
IYVCGNASKM AKDVEKALLD IISHNAHLNL EESQEFLNNL RLNKRYKRDV 600
Y 601
Length:601
Mass (Da):69,231
Last modified:December 1, 2000 - v1
Checksum:iA7BDD35F0CCF0E8F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13126.1.
RefSeqiNP_240240.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13126; BAB13126; BAB13126.
GeneIDi1109776.
KEGGibuc:BU428.
PATRICi21244396. VBIBucAph127364_0437.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13126.1 .
RefSeqi NP_240240.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57503.
SMRi P57503. Positions 229-601.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13126 ; BAB13126 ; BAB13126 .
GeneIDi 1109776.
KEGGi buc:BU428.
PATRICi 21244396. VBIBucAph127364_0437.

Phylogenomic databases

eggNOGi COG0369.
HOGENOMi HOG000282025.
KOi K00380.
OMAi HEFLQSK.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .
BioCyci BAPH107806:GBZJ-428-MONOMER.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPi MF_01541. CysJ.
InterProi IPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsi TIGR01931. cysJ. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiCYSJ_BUCAI
AccessioniPrimary (citable) accession number: P57503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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