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P57503

- CYSJ_BUCAI

UniProt

P57503 - CYSJ_BUCAI

Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

    Catalytic activityi

    H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

    Cofactori

    Binds 1 FAD per subunit.UniRule annotation
    Binds 1 FMN per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei491 – 4911NADPUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi71 – 755FMNUniRule annotation
    Nucleotide bindingi118 – 1236FMNUniRule annotation
    Nucleotide bindingi151 – 18232FMNUniRule annotationAdd
    BLAST
    Nucleotide bindingi388 – 3914FADUniRule annotation
    Nucleotide bindingi422 – 4243FADUniRule annotation
    Nucleotide bindingi521 – 5299NADPUniRule annotation

    GO - Molecular functioni

    1. flavin adenine dinucleotide binding Source: InterPro
    2. FMN binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. sulfite reductase (NADPH) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cysteine biosynthetic process Source: UniProtKB-KW
    2. hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
    3. sulfate assimilation Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

    Keywords - Ligandi

    FAD, Flavoprotein, FMN, NADP

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-428-MONOMER.
    UniPathwayiUPA00140; UER00207.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
    Short name:
    SiR-FPUniRule annotation
    Gene namesi
    Name:cysJUniRule annotation
    Ordered Locus Names:BU428
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 601601Sulfite reductase [NADPH] flavoprotein alpha-componentPRO_0000199920Add
    BLAST

    Interactioni

    Subunit structurei

    Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57503.
    SMRiP57503. Positions 229-601.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini65 – 203139Flavodoxin-likeUniRule annotationAdd
    BLAST
    Domaini236 – 450215FAD-binding FR-typeUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FAD-binding FR-type domain.UniRule annotation
    Contains 1 flavodoxin-like domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0369.
    HOGENOMiHOG000282025.
    KOiK00380.
    OMAiHEFLQSK.
    OrthoDBiEOG6CVV7G.

    Family and domain databases

    Gene3Di1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPiMF_01541. CysJ.
    InterProiIPR010199. CysJ.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view]
    PfamiPF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
    PRINTSiPR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMiSSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    TIGRFAMsiTIGR01931. cysJ. 1 hit.
    PROSITEiPS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57503-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKNQNKFDPV FPLSSEQLNN LKELERTCTN IQSAWLSGYF WKIANQTSNI    50
    TSFQTNESEK NDPVITIISA SQTGNAKLLS KRLYEYFNKN NKISRLIDAM 100
    DYKFKKIKDE KILILIISTQ GEGEPPEEAL SFYKFIMSKN APNLNNLYYS 150
    VFGLGDTSYN LFCQAGKDFD KRFKELGGNS LLDRFDADIE YEDNYNKWSQ 200
    DLLQSINSKE KIYKSSVSYI DQENTLILSK NHYTKKNPAE GIILTNQKIT 250
    GRNSKKDVHH IEIDISNLNI KYSPGDALGV WYKNDSNLVK NILELLSINI 300
    SETITIKNDV ITIFDALQNH FELTNNTKNI IKSYANFSKN KFLKDIISND 350
    SDLENYTINT PLIKMIHDHP LKLSSQQLIG LLRPLTPRLY SISSSQEEID 400
    NEIHITVGVV KKLISGHVYL GGASGYLSQS LKSDDIIKIF IQTNDNFRLP 450
    INKNTPIIMI SSGTGIAPFR AFMQQRDNDN ADGKNWLFFG NPNFTEDFLY 500
    QVEWQKYIKK GLITNMNLAW SQDQKNKIYV QDRIRENSQE IWSWIEEGAQ 550
    IYVCGNASKM AKDVEKALLD IISHNAHLNL EESQEFLNNL RLNKRYKRDV 600
    Y 601
    Length:601
    Mass (Da):69,231
    Last modified:December 1, 2000 - v1
    Checksum:iA7BDD35F0CCF0E8F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13126.1.
    RefSeqiNP_240240.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13126; BAB13126; BAB13126.
    GeneIDi1109776.
    KEGGibuc:BU428.
    PATRICi21244396. VBIBucAph127364_0437.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13126.1 .
    RefSeqi NP_240240.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57503.
    SMRi P57503. Positions 229-601.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13126 ; BAB13126 ; BAB13126 .
    GeneIDi 1109776.
    KEGGi buc:BU428.
    PATRICi 21244396. VBIBucAph127364_0437.

    Phylogenomic databases

    eggNOGi COG0369.
    HOGENOMi HOG000282025.
    KOi K00380.
    OMAi HEFLQSK.
    OrthoDBi EOG6CVV7G.

    Enzyme and pathway databases

    UniPathwayi UPA00140 ; UER00207 .
    BioCyci BAPH107806:GBZJ-428-MONOMER.

    Family and domain databases

    Gene3Di 1.20.990.10. 1 hit.
    3.40.50.360. 1 hit.
    HAMAPi MF_01541. CysJ.
    InterProi IPR010199. CysJ.
    IPR003097. FAD-binding_1.
    IPR017927. Fd_Rdtase_FAD-bd.
    IPR001094. Flavdoxin.
    IPR008254. Flavodoxin/NO_synth.
    IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
    IPR029039. Flavoprotein-like.
    IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
    IPR001433. OxRdtase_FAD/NAD-bd.
    IPR017938. Riboflavin_synthase-like_b-brl.
    [Graphical view ]
    Pfami PF00667. FAD_binding_1. 1 hit.
    PF00258. Flavodoxin_1. 1 hit.
    PF00175. NAD_binding_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
    PRINTSi PR00369. FLAVODOXIN.
    PR00371. FPNCR.
    SUPFAMi SSF52218. SSF52218. 1 hit.
    SSF63380. SSF63380. 1 hit.
    TIGRFAMsi TIGR01931. cysJ. 1 hit.
    PROSITEi PS51384. FAD_FR. 1 hit.
    PS50902. FLAVODOXIN_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiCYSJ_BUCAI
    AccessioniPrimary (citable) accession number: P57503
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3