Reviewed, UniProtKB/Swiss-Prot P57503 (CYSJ_BUCAI)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-component
      Short name=SIR-FP
    EC=1.8.1.2

Gene names

Name:	cysJ
Ordered Locus Names:	BU428

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavo-protein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01541
Cofactor	Binds 1 FAD per subunit By similarity. Binds 1 FMN per subunit By similarity.
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Electron transport Transport
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Sulfite reductase [NADPH] flavoprotein alpha-component HAMAP MF_01541		PRO_0000199920
Regions
	Domain	65 – 203	139	Flavodoxin-like
	Domain	236 – 450	215	FAD-binding FR-type
	Nucleotide binding	71 – 75	5	FMN By similarity
	Nucleotide binding	151 – 182	32	FMN By similarity
	Nucleotide binding	238 – 290	53	FAD By similarity
	Nucleotide binding	474 – 601	128	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57503-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: A7BDD35F0CCF0E8F	FASTA	601	69,231
	10 20 30 40 50 60 MKNQNKFDPV FPLSSEQLNN LKELERTCTN IQSAWLSGYF WKIANQTSNI TSFQTNESEK 70 80 90 100 110 120 NDPVITIISA SQTGNAKLLS KRLYEYFNKN NKISRLIDAM DYKFKKIKDE KILILIISTQ 130 140 150 160 170 180 GEGEPPEEAL SFYKFIMSKN APNLNNLYYS VFGLGDTSYN LFCQAGKDFD KRFKELGGNS 190 200 210 220 230 240 LLDRFDADIE YEDNYNKWSQ DLLQSINSKE KIYKSSVSYI DQENTLILSK NHYTKKNPAE 250 260 270 280 290 300 GIILTNQKIT GRNSKKDVHH IEIDISNLNI KYSPGDALGV WYKNDSNLVK NILELLSINI 310 320 330 340 350 360 SETITIKNDV ITIFDALQNH FELTNNTKNI IKSYANFSKN KFLKDIISND SDLENYTINT 370 380 390 400 410 420 PLIKMIHDHP LKLSSQQLIG LLRPLTPRLY SISSSQEEID NEIHITVGVV KKLISGHVYL 430 440 450 460 470 480 GGASGYLSQS LKSDDIIKIF IQTNDNFRLP INKNTPIIMI SSGTGIAPFR AFMQQRDNDN 490 500 510 520 530 540 ADGKNWLFFG NPNFTEDFLY QVEWQKYIKK GLITNMNLAW SQDQKNKIYV QDRIRENSQE 550 560 570 580 590 600 IWSWIEEGAQ IYVCGNASKM AKDVEKALLD IISHNAHLNL EESQEFLNNL RLNKRYKRDV Y

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13126.1.
RefSeq	NP_240240.1.
3D structure databases
HSSP	HSSP built from PDB template 1DDG based on UniProtKB P38038.
ModBase	Search...
Genome annotation databases
GeneID	1109776.
GenomeReviews	Gene locus BU428 in contig BA000003_GR.
KEGG	buc:BU428.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57503.
OMA	P57503. EQLAWVS.
Enzyme and pathway databases
BioCyc	BSP107806:BU428-MON.
Family and domain databases
HAMAP	MF_01541. [Tree]
InterPro	IPR010199. CysJ. IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001094. Flavdoxin-like. IPR008254. Flavodoxin/NO_synth. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
TIGRFAMs	TIGR01931. cysJ. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSJ_BUCAI

Accession

Primary (citable) accession number: P57503

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)