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P57503

- CYSJ_BUCAI

UniProt

P57503 - CYSJ_BUCAI

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Protein

Sulfite reductase [NADPH] flavoprotein alpha-component

Gene

cysJ

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component.UniRule annotation

Catalytic activityi

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • FADUniRule annotationNote: Binds 1 FAD per subunit.UniRule annotation
  • FMNUniRule annotationNote: Binds 1 FMN per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei491 – 4911NADPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi71 – 755FMNUniRule annotation
Nucleotide bindingi118 – 1236FMNUniRule annotation
Nucleotide bindingi151 – 18232FMNUniRule annotationAdd
BLAST
Nucleotide bindingi388 – 3914FADUniRule annotation
Nucleotide bindingi422 – 4243FADUniRule annotation
Nucleotide bindingi521 – 5299NADPUniRule annotation

GO - Molecular functioni

  1. flavin adenine dinucleotide binding Source: InterPro
  2. FMN binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. sulfite reductase (NADPH) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cysteine biosynthetic process Source: UniProtKB-KW
  2. hydrogen sulfide biosynthetic process Source: UniProtKB-HAMAP
  3. sulfate assimilation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-428-MONOMER.
UniPathwayiUPA00140; UER00207.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfite reductase [NADPH] flavoprotein alpha-componentUniRule annotation (EC:1.8.1.2UniRule annotation)
Short name:
SiR-FPUniRule annotation
Gene namesi
Name:cysJUniRule annotation
Ordered Locus Names:BU428
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 601601Sulfite reductase [NADPH] flavoprotein alpha-componentPRO_0000199920Add
BLAST

Interactioni

Subunit structurei

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57503.
SMRiP57503. Positions 229-601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 203139Flavodoxin-likeUniRule annotationAdd
BLAST
Domaini236 – 450215FAD-binding FR-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding FR-type domain.UniRule annotation
Contains 1 flavodoxin-like domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0369.
HOGENOMiHOG000282025.
KOiK00380.
OMAiHEFLQSK.
OrthoDBiEOG6CVV7G.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPiMF_01541. CysJ.
InterProiIPR010199. CysJ.
IPR029758. CysJ_Proteobact.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSiPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01931. cysJ. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57503-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNQNKFDPV FPLSSEQLNN LKELERTCTN IQSAWLSGYF WKIANQTSNI
60 70 80 90 100
TSFQTNESEK NDPVITIISA SQTGNAKLLS KRLYEYFNKN NKISRLIDAM
110 120 130 140 150
DYKFKKIKDE KILILIISTQ GEGEPPEEAL SFYKFIMSKN APNLNNLYYS
160 170 180 190 200
VFGLGDTSYN LFCQAGKDFD KRFKELGGNS LLDRFDADIE YEDNYNKWSQ
210 220 230 240 250
DLLQSINSKE KIYKSSVSYI DQENTLILSK NHYTKKNPAE GIILTNQKIT
260 270 280 290 300
GRNSKKDVHH IEIDISNLNI KYSPGDALGV WYKNDSNLVK NILELLSINI
310 320 330 340 350
SETITIKNDV ITIFDALQNH FELTNNTKNI IKSYANFSKN KFLKDIISND
360 370 380 390 400
SDLENYTINT PLIKMIHDHP LKLSSQQLIG LLRPLTPRLY SISSSQEEID
410 420 430 440 450
NEIHITVGVV KKLISGHVYL GGASGYLSQS LKSDDIIKIF IQTNDNFRLP
460 470 480 490 500
INKNTPIIMI SSGTGIAPFR AFMQQRDNDN ADGKNWLFFG NPNFTEDFLY
510 520 530 540 550
QVEWQKYIKK GLITNMNLAW SQDQKNKIYV QDRIRENSQE IWSWIEEGAQ
560 570 580 590 600
IYVCGNASKM AKDVEKALLD IISHNAHLNL EESQEFLNNL RLNKRYKRDV

Y
Length:601
Mass (Da):69,231
Last modified:December 1, 2000 - v1
Checksum:iA7BDD35F0CCF0E8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13126.1.
RefSeqiNP_240240.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13126; BAB13126; BAB13126.
GeneIDi1109776.
KEGGibuc:BU428.
PATRICi21244396. VBIBucAph127364_0437.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13126.1 .
RefSeqi NP_240240.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57503.
SMRi P57503. Positions 229-601.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13126 ; BAB13126 ; BAB13126 .
GeneIDi 1109776.
KEGGi buc:BU428.
PATRICi 21244396. VBIBucAph127364_0437.

Phylogenomic databases

eggNOGi COG0369.
HOGENOMi HOG000282025.
KOi K00380.
OMAi HEFLQSK.
OrthoDBi EOG6CVV7G.

Enzyme and pathway databases

UniPathwayi UPA00140 ; UER00207 .
BioCyci BAPH107806:GBZJ-428-MONOMER.

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
HAMAPi MF_01541. CysJ.
InterProi IPR010199. CysJ.
IPR029758. CysJ_Proteobact.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSi PR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsi TIGR01931. cysJ. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiCYSJ_BUCAI
AccessioniPrimary (citable) accession number: P57503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 26, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3