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P57503 (CYSJ_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Sulfite reductase [NADPH] flavoprotein alpha-component
Short name=SiR-FP
EC=1.8.1.2
Gene names
Name:cysJ
Ordered Locus Names:BU428
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera ›

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity. HAMAP-Rule MF_01541

Catalytic activity

H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP-Rule MF_01541

Cofactor

Binds 1 FAD per subunit By similarity.

Binds 1 FMN per subunit By similarity.

Pathway

Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP-Rule MF_01541

Subunit structure

Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.

Sequence similarities

Contains 1 FAD-binding FR-type domain.

Contains 1 flavodoxin-like domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 601601Sulfite reductase [NADPH] flavoprotein alpha-component HAMAP-Rule MF_01541
PRO_0000199920

Regions

Domain65 – 203139Flavodoxin-like
Domain236 – 450215FAD-binding FR-type
Nucleotide binding71 – 755FMN By similarity
Nucleotide binding118 – 1236FMN By similarity
Nucleotide binding151 – 18232FMN By similarity
Nucleotide binding388 – 3914FAD By similarity
Nucleotide binding422 – 4243FAD By similarity
Nucleotide binding521 – 5299NADP By similarity

Sites

Binding site4911NADP By similarity

Sequences

Sequence LengthMass (Da)Tools
P57503 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: A7BDD35F0CCF0E8F

FASTA60169,231
        10         20         30         40         50         60 
MKNQNKFDPV FPLSSEQLNN LKELERTCTN IQSAWLSGYF WKIANQTSNI TSFQTNESEK 

        70         80         90        100        110        120 
NDPVITIISA SQTGNAKLLS KRLYEYFNKN NKISRLIDAM DYKFKKIKDE KILILIISTQ 

       130        140        150        160        170        180 
GEGEPPEEAL SFYKFIMSKN APNLNNLYYS VFGLGDTSYN LFCQAGKDFD KRFKELGGNS 

       190        200        210        220        230        240 
LLDRFDADIE YEDNYNKWSQ DLLQSINSKE KIYKSSVSYI DQENTLILSK NHYTKKNPAE 

       250        260        270        280        290        300 
GIILTNQKIT GRNSKKDVHH IEIDISNLNI KYSPGDALGV WYKNDSNLVK NILELLSINI 

       310        320        330        340        350        360 
SETITIKNDV ITIFDALQNH FELTNNTKNI IKSYANFSKN KFLKDIISND SDLENYTINT 

       370        380        390        400        410        420 
PLIKMIHDHP LKLSSQQLIG LLRPLTPRLY SISSSQEEID NEIHITVGVV KKLISGHVYL 

       430        440        450        460        470        480 
GGASGYLSQS LKSDDIIKIF IQTNDNFRLP INKNTPIIMI SSGTGIAPFR AFMQQRDNDN 

       490        500        510        520        530        540 
ADGKNWLFFG NPNFTEDFLY QVEWQKYIKK GLITNMNLAW SQDQKNKIYV QDRIRENSQE 

       550        560        570        580        590        600 
IWSWIEEGAQ IYVCGNASKM AKDVEKALLD IISHNAHLNL EESQEFLNNL RLNKRYKRDV 


Y

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References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB13126.1.
RefSeqNP_240240.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57503.
SMRP57503. Positions 229-601.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13126; BAB13126; BAB13126.
GeneID1109776.
KEGGbuc:BU428.
PATRIC21244396. VBIBucAph127364_0437.

Phylogenomic databases

eggNOGCOG0369.
HOGENOMHOG000282025.
KOK00380.
OMASDKDVRH.
ProtClustDBCLSK316168.

Enzyme and pathway databases

UniPathwayUPA00140; UER00207.

Family and domain databases

Gene3D1.20.990.10. 1 hit.
HAMAPMF_01541. CysJ.
InterProIPR010199. CysJ.
IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001094. Flavdoxin.
IPR008254. Flavodoxin/NO_synth.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamPF00667. FAD_binding_1. 1 hit.
PF00258. Flavodoxin_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFPIRSF000207. SiR-FP_CysJ. 1 hit.
PRINTSPR00369. FLAVODOXIN.
PR00371. FPNCR.
SUPFAMSSF63380. Riboflavin_synthase_like_b-brl. 1 hit.
TIGRFAMsTIGR01931. cysJ. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
PS50902. FLAVODOXIN_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCYSJ_BUCAI
AccessionPrimary (citable) accession number: P57503
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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