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Protein

Phosphoadenosine phosphosulfate reductase

Gene

cysH

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reduction of activated sulfate into sulfite.By similarity

Catalytic activityi

Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.

Pathway: hydrogen sulfide biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-426-MONOMER.
UniPathwayiUPA00140; UER00206.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoadenosine phosphosulfate reductase (EC:1.8.4.8)
Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase
Gene namesi
Name:cysH
Ordered Locus Names:BU426
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 244244Phosphoadenosine phosphosulfate reductasePRO_0000100628Add
BLAST

Proteomic databases

PRIDEiP57501.

Interactioni

Protein-protein interaction databases

STRINGi107806.BU426.

Structurei

3D structure databases

ProteinModelPortaliP57501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PAPS reductase family. CysH subfamily.Curated

Phylogenomic databases

eggNOGiCOG0175.
HOGENOMiHOG000249396.
KOiK00390.
OMAiPRNKLLD.
OrthoDBiEOG6V1M7D.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00063. CysH.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.

Sequencei

Sequence statusi: Complete.

P57501-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFYIENIN LLNSEKKNNI LSELNLLLSN YSAEERISWA LSHLPHTQIM
60 70 80 90 100
SSSFGIQSTV LLHLIIKKKP DIPVILIDTG YLFPETYNFI DFLTNKFHLN
110 120 130 140 150
LKVFRSTISS AWQEARYGKL WEKGIEGIDF YNNINKVQPM NFALNELSVQ
160 170 180 190 200
TWFAGLRHDQ SKSRNLLPYL SIKKGIFKIL PILDWSKDKI KDYLKENNLD
210 220 230 240
THPLYNNGYS SVGDTHTTKK HMPGMLEEET RFFGLKRECG LHEN
Length:244
Mass (Da):28,402
Last modified:December 1, 2000 - v1
Checksum:iED7ADB9778927DDB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13124.1.
RefSeqiNP_240238.1. NC_002528.1.
WP_010896110.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13124; BAB13124; BAB13124.
GeneIDi1109774.
KEGGibuc:BU426.
PATRICi21244392. VBIBucAph127364_0435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13124.1.
RefSeqiNP_240238.1. NC_002528.1.
WP_010896110.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU426.

Proteomic databases

PRIDEiP57501.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13124; BAB13124; BAB13124.
GeneIDi1109774.
KEGGibuc:BU426.
PATRICi21244392. VBIBucAph127364_0435.

Phylogenomic databases

eggNOGiCOG0175.
HOGENOMiHOG000249396.
KOiK00390.
OMAiPRNKLLD.
OrthoDBiEOG6V1M7D.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00206.
BioCyciBAPH107806:GBZJ-426-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00063. CysH.
InterProiIPR004511. PAPS/APS_Rdtase.
IPR002500. PAPS_reduct.
IPR011800. PAPS_reductase_CysH.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
[Graphical view]
PIRSFiPIRSF000857. PAPS_reductase. 1 hit.
TIGRFAMsiTIGR00434. cysH. 1 hit.
TIGR02057. PAPS_reductase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiCYSH_BUCAI
AccessioniPrimary (citable) accession number: P57501
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 24, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.