P57500 (CYSG_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Siroheme synthase

Including the following 3 domains:

1. Uroporphyrinogen-III C-methyltransferase
   Short name=Urogen III methylase
   EC=2.1.1.107
   Alternative name(s):
   SUMT
   Uroporphyrinogen III methylase
   Short name=UROM
2. Precorrin-2 dehydrogenase
   EC=1.3.1.76
3. Sirohydrochlorin ferrochelatase
   EC=4.99.1.4

Gene names

Name:	cysG
Ordered Locus Names:	BU425

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP-Rule MF_01646
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP-Rule MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP-Rule MF_01646 Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP-Rule MF_01646 Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP-Rule MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP-Rule MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP-Rule MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	cobalamin biosynthetic process Inferred from electronic annotation. Source: HAMAP siroheme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: HAMAP sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Siroheme synthase HAMAP-Rule MF_01646		PRO_0000150377
Regions
	Region	217 – 457	241	Uroporphyrinogen-III C-methyltransferase HAMAP-Rule MF_01646

Sequences

	Sequence		Length	Mass (Da)
	P57500 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 4B5781BC2AA565BA	FASTA	473	52,874
	10 20 30 40 50 60 MNYLPIFIDL KSKNVLVIGA GEVGLNKIRI LLRAKAKVNV IAKELCSEVK LLLRDQKINW 70 80 90 100 110 120 LSKNFDLIYL NKIFLVVSAT NDIKLNQYIF KKCNERCVLV NIVDDKLKCS FIFPSIIDRS 130 140 150 160 170 180 PLIVAISSGG TAPVLLRLLR EKIEAILPNK LGDVAKIAGK WRLAIKKHFS NFLERRKFWE 190 200 210 220 230 240 KLFHSIFVEH ILNGNKEQAI NVLKKNMNQN ISLTGEIILV GAGPGDSGLL TLRGLQVLQQ 250 260 270 280 290 300 ADVVLYDYLV SEDILDLIRR DAKRICVGKR VGLKNITQNE IIKLLIFFAQ KGKKVVRLKG 310 320 330 340 350 360 GDPFIFGRGS EEIEAAKDAG IHFQVVPGIT SAIGIAAYTG IPLTHRKYSQ GVIFITGHKC 370 380 390 400 410 420 IDGFLNNWSI LSDPSYTLVV YMGTLKAVYI AQKLITFGRS KLTPIAIIVQ GTTIHQKVIV 430 440 450 460 470 GCLGEIEKII PFAATPSLLI IGDVVHLHKK LAWFQSENIL KKIKNKFSTL TFI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13123.1.
RefSeq	NP_240237.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57500.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13123; BAB13123; BAB13123.
GeneID	1109773.
KEGG	buc:BU425.
PATRIC	21244390. VBIBucAph127364_0434.
Phylogenomic databases
eggNOG	COG0007.
HOGENOM	HOG000290518.
KO	K02302.
OMA	IAVYMGR.
ProtClustDB	CLSK2459979.
Enzyme and pathway databases
UniPathway	UPA00148; UER00211. UPA00148; UER00222. UPA00262; UER00211. UPA00262; UER00222. UPA00262; UER00376.
Family and domain databases
Gene3D	1.10.8.210. 1 hit. 3.30.950.10. 1 hit. 3.40.1010.10. 1 hit. 3.40.50.720. 1 hit.
HAMAP	MF_01646. Siroheme_synth.
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA/CysG_C. IPR016040. NAD(P)-bd_dom. IPR012409. Sirohaem_synth. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Pfam	PF10414. CysG_dimeriser. 1 hit. PF13241. NAD_binding_7. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAM	SSF53790. Cor/por_Metransf. 1 hit.
TIGRFAMs	TIGR01469. cobA_cysG_Cterm. 1 hit. TIGR01470. cysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSG_BUCAI

Accession

Primary (citable) accession number: P57500

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program