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P57492

- ENO_BUCAI

UniProt

P57492 - ENO_BUCAI

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Protein

Enolase

Gene
eno, BU417
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis By similarity.UniRule annotation

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei159 – 1591Substrate By similarity
Binding sitei168 – 1681Substrate By similarity
Active sitei209 – 2091Proton donor By similarity
Metal bindingi246 – 2461Magnesium By similarity
Metal bindingi291 – 2911Magnesium By similarity
Binding sitei291 – 2911Substrate By similarity
Metal bindingi318 – 3181Magnesium By similarity
Binding sitei318 – 3181Substrate By similarity
Active sitei343 – 3431Proton acceptor By similarity
Binding sitei343 – 3431Substrate (covalent); in inhibited form By similarity
Binding sitei394 – 3941Substrate By similarity

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-417-MONOMER.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:eno
Ordered Locus Names:BU417
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity. Secreted By similarity. Cell surface By similarity
Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface By similarity.UniRule annotation

GO - Cellular componenti

  1. cell surface Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-SubCell
  3. phosphopyruvate hydratase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 434434EnolaseUniRule annotationPRO_0000133855Add
BLAST

Proteomic databases

PRIDEiP57492.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57492.
SMRiP57492. Positions 2-431.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni370 – 3734Substrate binding By similarity

Sequence similaritiesi

Belongs to the enolase family.

Phylogenomic databases

eggNOGiCOG0148.
HOGENOMiHOG000072174.
KOiK01689.
OMAiHANWQGA.
OrthoDBiEOG65J589.

Family and domain databases

Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57492-1 [UniParc]FASTAAdd to Basket

« Hide

MSKITKIIAR EIIDSRGNPT VESEVHLEGG FVGLASSPSG ASTGSLEALE    50
LRDENKDRFM GKGVEKAVSL INEKISIALK NKNARNQSDI DHIMIDLDGT 100
INKSKLGANA ILSVSLAVAK AAAASKRMPL YAHIAEINET PGVFSMPLPM 150
INIINGGKHA NNNIDIQEFM IQPISAKTVK ESIRIGCEIF HALGELLKEK 200
GMSTTVGDEG GYAPNLKSNE EALNIIQDAI QKTKYKLGQD IRLAIDCAAS 250
ELYNKNEKKY NLKGENISFS SKEFTHYLEK LSQKYPIVSI EDGQDESDWE 300
GFLYQTHVLG NKIQLVGDDL FVTNKNILKK GIKKGIANSI LIKLNQIGTL 350
TETLEAIKTA KQANYGVIIS HRSGETEDAS IADLSVGTSS GQIKTGSMSR 400
SDRTSKYNQL IRIEENLGTK YAPFHGLREI KSAF 434
Length:434
Mass (Da):47,428
Last modified:December 1, 2000 - v1
Checksum:iD3F5FD25D8F52D40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13115.1.
RefSeqiNP_240229.1. NC_002528.1.
WP_010896105.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13115; BAB13115; BAB13115.
GeneIDi1109765.
KEGGibuc:BU417.
PATRICi21244374. VBIBucAph127364_0426.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13115.1 .
RefSeqi NP_240229.1. NC_002528.1.
WP_010896105.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57492.
SMRi P57492. Positions 2-431.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P57492.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13115 ; BAB13115 ; BAB13115 .
GeneIDi 1109765.
KEGGi buc:BU417.
PATRICi 21244374. VBIBucAph127364_0426.

Phylogenomic databases

eggNOGi COG0148.
HOGENOMi HOG000072174.
KOi K01689.
OMAi HANWQGA.
OrthoDBi EOG65J589.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00187 .
BioCyci BAPH107806:GBZJ-417-MONOMER.

Family and domain databases

Gene3Di 3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPi MF_00318. Enolase.
InterProi IPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N_like.
[Graphical view ]
PANTHERi PTHR11902. PTHR11902. 1 hit.
Pfami PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF001400. Enolase. 1 hit.
PRINTSi PR00148. ENOLASE.
SUPFAMi SSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsi TIGR01060. eno. 1 hit.
PROSITEi PS00164. ENOLASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiENO_BUCAI
AccessioniPrimary (citable) accession number: P57492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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