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P57492

- ENO_BUCAI

UniProt

P57492 - ENO_BUCAI

Protein

Enolase

Gene

eno

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible conversion of 2-phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis.UniRule annotation

    Catalytic activityi

    2-phospho-D-glycerate = phosphoenolpyruvate + H2O.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    The covalent binding to the substrate causes inactivation of the enzyme, and possibly serves as a signal for the export of the protein.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei159 – 1591SubstrateUniRule annotation
    Binding sitei168 – 1681SubstrateUniRule annotation
    Active sitei209 – 2091Proton donorUniRule annotation
    Metal bindingi246 – 2461MagnesiumUniRule annotation
    Metal bindingi291 – 2911MagnesiumUniRule annotation
    Binding sitei291 – 2911SubstrateUniRule annotation
    Metal bindingi318 – 3181MagnesiumUniRule annotation
    Binding sitei318 – 3181SubstrateUniRule annotation
    Active sitei343 – 3431Proton acceptorUniRule annotation
    Binding sitei343 – 3431Substrate (covalent); in inhibited formUniRule annotation
    Binding sitei394 – 3941SubstrateUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. phosphopyruvate hydratase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-417-MONOMER.
    UniPathwayiUPA00109; UER00187.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EnolaseUniRule annotation (EC:4.2.1.11UniRule annotation)
    Alternative name(s):
    2-phospho-D-glycerate hydro-lyaseUniRule annotation
    2-phosphoglycerate dehydrataseUniRule annotation
    Gene namesi
    Name:enoUniRule annotation
    Ordered Locus Names:BU417
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation. Secreted UniRule annotation. Cell surface UniRule annotation
    Note: Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface.UniRule annotation

    GO - Cellular componenti

    1. cell surface Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-SubCell
    3. phosphopyruvate hydratase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 434434EnolasePRO_0000133855Add
    BLAST

    Proteomic databases

    PRIDEiP57492.

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57492.
    SMRiP57492. Positions 2-431.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni370 – 3734Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the enolase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0148.
    HOGENOMiHOG000072174.
    KOiK01689.
    OMAiHANWQGA.
    OrthoDBiEOG65J589.

    Family and domain databases

    Gene3Di3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPiMF_00318. Enolase.
    InterProiIPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view]
    PANTHERiPTHR11902. PTHR11902. 1 hit.
    PfamiPF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001400. Enolase. 1 hit.
    PRINTSiPR00148. ENOLASE.
    SUPFAMiSSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsiTIGR01060. eno. 1 hit.
    PROSITEiPS00164. ENOLASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57492-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSKITKIIAR EIIDSRGNPT VESEVHLEGG FVGLASSPSG ASTGSLEALE    50
    LRDENKDRFM GKGVEKAVSL INEKISIALK NKNARNQSDI DHIMIDLDGT 100
    INKSKLGANA ILSVSLAVAK AAAASKRMPL YAHIAEINET PGVFSMPLPM 150
    INIINGGKHA NNNIDIQEFM IQPISAKTVK ESIRIGCEIF HALGELLKEK 200
    GMSTTVGDEG GYAPNLKSNE EALNIIQDAI QKTKYKLGQD IRLAIDCAAS 250
    ELYNKNEKKY NLKGENISFS SKEFTHYLEK LSQKYPIVSI EDGQDESDWE 300
    GFLYQTHVLG NKIQLVGDDL FVTNKNILKK GIKKGIANSI LIKLNQIGTL 350
    TETLEAIKTA KQANYGVIIS HRSGETEDAS IADLSVGTSS GQIKTGSMSR 400
    SDRTSKYNQL IRIEENLGTK YAPFHGLREI KSAF 434
    Length:434
    Mass (Da):47,428
    Last modified:December 1, 2000 - v1
    Checksum:iD3F5FD25D8F52D40
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13115.1.
    RefSeqiNP_240229.1. NC_002528.1.
    WP_010896105.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13115; BAB13115; BAB13115.
    GeneIDi1109765.
    KEGGibuc:BU417.
    PATRICi21244374. VBIBucAph127364_0426.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13115.1 .
    RefSeqi NP_240229.1. NC_002528.1.
    WP_010896105.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57492.
    SMRi P57492. Positions 2-431.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P57492.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13115 ; BAB13115 ; BAB13115 .
    GeneIDi 1109765.
    KEGGi buc:BU417.
    PATRICi 21244374. VBIBucAph127364_0426.

    Phylogenomic databases

    eggNOGi COG0148.
    HOGENOMi HOG000072174.
    KOi K01689.
    OMAi HANWQGA.
    OrthoDBi EOG65J589.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00187 .
    BioCyci BAPH107806:GBZJ-417-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.120. 1 hit.
    3.30.390.10. 1 hit.
    HAMAPi MF_00318. Enolase.
    InterProi IPR000941. Enolase.
    IPR020810. Enolase_C.
    IPR029065. Enolase_C-like.
    IPR020809. Enolase_CS.
    IPR020811. Enolase_N.
    IPR029017. Enolase_N_like.
    [Graphical view ]
    PANTHERi PTHR11902. PTHR11902. 1 hit.
    Pfami PF00113. Enolase_C. 1 hit.
    PF03952. Enolase_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001400. Enolase. 1 hit.
    PRINTSi PR00148. ENOLASE.
    SUPFAMi SSF51604. SSF51604. 1 hit.
    SSF54826. SSF54826. 1 hit.
    TIGRFAMsi TIGR01060. eno. 1 hit.
    PROSITEi PS00164. ENOLASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiENO_BUCAI
    AccessioniPrimary (citable) accession number: P57492
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3