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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei272 – 2721ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-415-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:glnS
Ordered Locus Names:BU415
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Glutamine--tRNA ligasePRO_0000195827Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi107806.BU415.

Structurei

3D structure databases

ProteinModelPortaliP57490.
SMRiP57490. Positions 10-557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi35 – 4511"HIGH" regionAdd
BLAST
Motifi269 – 2735"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiLRDSWAK.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTKKEIKNN NFICQIINKD LNENKNLSFY TRFPPEPNGY LHIGHAKSIC
60 70 80 90 100
LNFELASLYK GRCNLRFDDT NPLKENIKYI ESIKHDINWL GYKWHGNVRY
110 120 130 140 150
ASEYFLKLYQ YAQELIKKGL AYVDHLTKEQ IREYRGTLNT PGKNSPYRNR
160 170 180 190 200
TIQENIELFE KMKKGDFSEG EACLRAKINM SSSSIIMRDP VLYRIIFIKH
210 220 230 240 250
HQTQNKWCIY PMYDFAHCLS DSIEGITHSL CTLEFQDNKF LYNWILKNTS
260 270 280 290 300
VKHYPKQYEF SRLNLEFSIL SKRKIKILID KNIIEGWDDP RIPTLSALRR
310 320 330 340 350
KGYTPSSIKN FCQKIGVTKQ NNLIEFSMLE HCIRKELNQT AIRTMAILDP
360 370 380 390 400
IKIFLYNLDS NYKEEFIVPN HPNNPEMGTH KIIFTNTIYI DRSDFKEKYD
410 420 430 440 450
KKYKRLKLGE KIRLRYSYII HAEKIEKDEY GNISNIICYC DLNTLGRKPK
460 470 480 490 500
DNKNPAVIHW ISEKNTLSAE FKLYDQLFNI KNPEQQENFL LYINSKSLIK
510 520 530 540 550
KFGFIEKKIG EEIQKKISNN NIEIFFQFER IGYFCIDFID SKKNQLVFNR
560 570
TVGLRDTWDS KKIKTKNITN N
Length:571
Mass (Da):67,835
Last modified:December 1, 2000 - v1
Checksum:i1FCE69932D563D52
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13113.1.
RefSeqiNP_240227.1. NC_002528.1.
WP_010896103.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13113; BAB13113; BAB13113.
GeneIDi1109763.
KEGGibuc:BU415.
PATRICi21244370. VBIBucAph127364_0424.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13113.1.
RefSeqiNP_240227.1. NC_002528.1.
WP_010896103.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57490.
SMRiP57490. Positions 10-557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU415.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13113; BAB13113; BAB13113.
GeneIDi1109763.
KEGGibuc:BU415.
PATRICi21244370. VBIBucAph127364_0424.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OMAiLRDSWAK.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-415-MONOMER.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiSYQ_BUCAI
AccessioniPrimary (citable) accession number: P57490
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 22, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.