Reviewed, UniProtKB/Swiss-Prot P57490 (SYQ_BUCAI)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	BU415

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000195827
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP MF_00126
	Motif	269 – 273	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	272	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57490-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 1FCE69932D563D52	FASTA	571	67,835
	10 20 30 40 50 60 MDTKKEIKNN NFICQIINKD LNENKNLSFY TRFPPEPNGY LHIGHAKSIC LNFELASLYK 70 80 90 100 110 120 GRCNLRFDDT NPLKENIKYI ESIKHDINWL GYKWHGNVRY ASEYFLKLYQ YAQELIKKGL 130 140 150 160 170 180 AYVDHLTKEQ IREYRGTLNT PGKNSPYRNR TIQENIELFE KMKKGDFSEG EACLRAKINM 190 200 210 220 230 240 SSSSIIMRDP VLYRIIFIKH HQTQNKWCIY PMYDFAHCLS DSIEGITHSL CTLEFQDNKF 250 260 270 280 290 300 LYNWILKNTS VKHYPKQYEF SRLNLEFSIL SKRKIKILID KNIIEGWDDP RIPTLSALRR 310 320 330 340 350 360 KGYTPSSIKN FCQKIGVTKQ NNLIEFSMLE HCIRKELNQT AIRTMAILDP IKIFLYNLDS 370 380 390 400 410 420 NYKEEFIVPN HPNNPEMGTH KIIFTNTIYI DRSDFKEKYD KKYKRLKLGE KIRLRYSYII 430 440 450 460 470 480 HAEKIEKDEY GNISNIICYC DLNTLGRKPK DNKNPAVIHW ISEKNTLSAE FKLYDQLFNI 490 500 510 520 530 540 KNPEQQENFL LYINSKSLIK KFGFIEKKIG EEIQKKISNN NIEIFFQFER IGYFCIDFID 550 560 570 SKKNQLVFNR TVGLRDTWDS KKIKTKNITN N

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13113.1.
RefSeq	NP_240227.1.
3D structure databases
HSSP	HSSP built from PDB template 1NYL based on UniProtKB P00962.
ModBase	Search...
Genome annotation databases
GeneID	1109763.
GenomeReviews	Gene locus BU415 in contig BA000003_GR.
KEGG	buc:BU415.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57490.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	BSP107806:BU415-MON.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_BUCAI

Accession

Primary (citable) accession number: P57490

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)