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P57490 (SYQ_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:BU415
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length571 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 571571Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_0000195827

Regions

Motif35 – 4511"HIGH" region HAMAP-Rule MF_00126
Motif269 – 2735"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2721ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P57490 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 1FCE69932D563D52

FASTA57167,835
        10         20         30         40         50         60 
MDTKKEIKNN NFICQIINKD LNENKNLSFY TRFPPEPNGY LHIGHAKSIC LNFELASLYK 

        70         80         90        100        110        120 
GRCNLRFDDT NPLKENIKYI ESIKHDINWL GYKWHGNVRY ASEYFLKLYQ YAQELIKKGL 

       130        140        150        160        170        180 
AYVDHLTKEQ IREYRGTLNT PGKNSPYRNR TIQENIELFE KMKKGDFSEG EACLRAKINM 

       190        200        210        220        230        240 
SSSSIIMRDP VLYRIIFIKH HQTQNKWCIY PMYDFAHCLS DSIEGITHSL CTLEFQDNKF 

       250        260        270        280        290        300 
LYNWILKNTS VKHYPKQYEF SRLNLEFSIL SKRKIKILID KNIIEGWDDP RIPTLSALRR 

       310        320        330        340        350        360 
KGYTPSSIKN FCQKIGVTKQ NNLIEFSMLE HCIRKELNQT AIRTMAILDP IKIFLYNLDS 

       370        380        390        400        410        420 
NYKEEFIVPN HPNNPEMGTH KIIFTNTIYI DRSDFKEKYD KKYKRLKLGE KIRLRYSYII 

       430        440        450        460        470        480 
HAEKIEKDEY GNISNIICYC DLNTLGRKPK DNKNPAVIHW ISEKNTLSAE FKLYDQLFNI 

       490        500        510        520        530        540 
KNPEQQENFL LYINSKSLIK KFGFIEKKIG EEIQKKISNN NIEIFFQFER IGYFCIDFID 

       550        560        570 
SKKNQLVFNR TVGLRDTWDS KKIKTKNITN N 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13113.1.
RefSeqNP_240227.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57490.
SMRP57490. Positions 10-557.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13113; BAB13113; BAB13113.
GeneID1109763.
KEGGbuc:BU415.
PATRIC21244370. VBIBucAph127364_0424.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMAVTHSICT.
OrthoDBEOG6DRPF7.
ProtClustDBPRK05347.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-415-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_BUCAI
AccessionPrimary (citable) accession number: P57490
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries