P57488 (RSME_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase E
EC=2.1.1.193

Alternative name(s):
16S rRNA m3U1498 methyltransferase

Gene names

Name:	rsmE
Ordered Locus Names:	BU410

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit By similarity.
Catalytic activity	S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the RNA methyltransferase RsmE family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Ribosomal RNA small subunit methyltransferase E		PRO_0000176208

Sequences

	Sequence		Length	Mass (Da)
	P57488 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: D56AEB51B760AFD6	FASTA	253	29,962
	10 20 30 40 50 60 MFTSTAIKNK KIPRIYIEDD LDLNQFYFLS DENRHYVTKV LRMKIEDILE IFNNTHYIFF 70 80 90 100 110 120 AKIIDISKKI IKIQTFKKIL KNLESPLHIH LGQVISKNEK MDFTIQKSIE IGVKTITPLF 130 140 150 160 170 180 FENDHFQKKR INFSNKIKRW EKIAISACRQ CNRNIIPKIK IPMNVFHWCE NNQNNDKKII 190 200 210 220 230 240 FHPKSTLTMK CLTEPIKYIQ IIIGSERGFF NDEFQKIIKY GFIPIRLGPR ILRTETASVV 250 AITALQTMFG DFK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13111.1.
RefSeq	NP_240225.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57488.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13111; BAB13111; BAB13111.
GeneID	1109761.
KEGG	buc:BU410.
PATRIC	21244360. VBIBucAph127364_0422.
Phylogenomic databases
eggNOG	COG1385.
HOGENOM	HOG000015265.
KO	K09761.
OMA	QENFFIL.
ProtClustDB	PRK11713.
Family and domain databases
InterPro	IPR015947. PUA-like_domain. IPR006700. rRNA_ssu_MeTrfase-E. [Graphical view]
Pfam	PF04452. Methyltrans_RNA. 1 hit. [Graphical view]
PIRSF	PIRSF015601. MTase_slr0722. 1 hit.
SUPFAM	SSF88697. PUA-like. 1 hit.
TIGRFAMs	TIGR00046. TIGR00046. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSME_BUCAI

Accession

Primary (citable) accession number: P57488

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program