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Protein

Glutamate--cysteine ligase

Gene

gshA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. glutamate-cysteine ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutathione biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Glutathione biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-407-MONOMER.
UniPathwayiUPA00142; UER00209.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--cysteine ligase (EC:6.3.2.2)
Alternative name(s):
Gamma-ECS
Short name:
GCS
Gamma-glutamylcysteine synthetase
Gene namesi
Name:gshA
Ordered Locus Names:BU407
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Glutamate--cysteine ligasePRO_0000192520Add
BLAST

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2918.
HOGENOMiHOG000266224.
KOiK01919.
OMAiQTISGIH.
OrthoDBiEOG6BKJ7H.

Family and domain databases

HAMAPiMF_00578. Glu_cys_ligase.
InterProiIPR007370. Glu_cys_ligase.
IPR006334. Glut_cys_ligase.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01434. glu_cys_ligase. 1 hit.

Sequencei

Sequence statusi: Complete.

P57485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEDISKKIA WLKKNPKMLK GIFRGIERET LRIQKNGHFS KTIHPYLIGS
60 70 80 90 100
SLTHKWITTD FSENLLEFIT PTSDNIDYLL SFLTDLHSFT ASKIKNERMW
110 120 130 140 150
PFSIPYCFND QTNIQIAQYG KSNIGKMKTT YRIGLKNRYG DLINTISGIH
160 170 180 190 200
YNFSLPLFFW TNWENNQNKK NNTDLISSGY LNLIRNYYRF GWIVPYLFGS
210 220 230 240 250
SPAISSFFLK NTKKKYKFKK NKEDIFYLPW STSLRLSDIG YSNTNILDLN
260 270 280 290 300
IMFNDFNEYI ESFQNALKTP SKKFINIGLK DEHGNFKQLN TNILQIENEL
310 320 330 340 350
YTQIRPKRKT KDGESLLEAL KNRGIEYVEI RSLDVNPFSP IGINKNQILL
360 370 380 390 400
LDLFLIWCAL IDSPKIDKTD FLLTTKNWER IIYEGRKPNQ KIYINNNNET
410 420 430 440 450
KTLIEIGQII FKDLNEIALI LDSNSNDLLY QKACKETQLF LKNPELTYSA
460 470 480 490 500
QCLNFLMTTG IKKTGLYLAN KYHEKFINKN YFNLNQSVLE QEVIRSHQKK
510
IEIEREDILS FEEYIRNK
Length:518
Mass (Da):60,979
Last modified:December 1, 2000 - v1
Checksum:iAC6659F1E2C50BD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13108.1.
RefSeqiNP_240222.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13108; BAB13108; BAB13108.
GeneIDi1109758.
KEGGibuc:BU407.
PATRICi21244354. VBIBucAph127364_0419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13108.1.
RefSeqiNP_240222.1. NC_002528.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13108; BAB13108; BAB13108.
GeneIDi1109758.
KEGGibuc:BU407.
PATRICi21244354. VBIBucAph127364_0419.

Phylogenomic databases

eggNOGiCOG2918.
HOGENOMiHOG000266224.
KOiK01919.
OMAiQTISGIH.
OrthoDBiEOG6BKJ7H.

Enzyme and pathway databases

UniPathwayiUPA00142; UER00209.
BioCyciBAPH107806:GBZJ-407-MONOMER.

Family and domain databases

HAMAPiMF_00578. Glu_cys_ligase.
InterProiIPR007370. Glu_cys_ligase.
IPR006334. Glut_cys_ligase.
[Graphical view]
PfamiPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01434. glu_cys_ligase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiGSH1_BUCAI
AccessioniPrimary (citable) accession number: P57485
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 1, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.