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P57485 (GSH1_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--cysteine ligase

EC=6.3.2.2
Alternative name(s):
Gamma-ECS
Short name=GCS
Gamma-glutamylcysteine synthetase
Gene names
Name:gshA
Ordered Locus Names:BU407
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length518 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine. HAMAP-Rule MF_00578

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2. HAMAP-Rule MF_00578

Sequence similarities

Belongs to the glutamate--cysteine ligase type 1 family. Type 1 subfamily.

Ontologies

Keywords
   Biological processGlutathione biosynthesis
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutathione biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-cysteine ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 518518Glutamate--cysteine ligase HAMAP-Rule MF_00578
PRO_0000192520

Sequences

Sequence LengthMass (Da)Tools
P57485 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: AC6659F1E2C50BD4

FASTA51860,979
        10         20         30         40         50         60 
MIEDISKKIA WLKKNPKMLK GIFRGIERET LRIQKNGHFS KTIHPYLIGS SLTHKWITTD 

        70         80         90        100        110        120 
FSENLLEFIT PTSDNIDYLL SFLTDLHSFT ASKIKNERMW PFSIPYCFND QTNIQIAQYG 

       130        140        150        160        170        180 
KSNIGKMKTT YRIGLKNRYG DLINTISGIH YNFSLPLFFW TNWENNQNKK NNTDLISSGY 

       190        200        210        220        230        240 
LNLIRNYYRF GWIVPYLFGS SPAISSFFLK NTKKKYKFKK NKEDIFYLPW STSLRLSDIG 

       250        260        270        280        290        300 
YSNTNILDLN IMFNDFNEYI ESFQNALKTP SKKFINIGLK DEHGNFKQLN TNILQIENEL 

       310        320        330        340        350        360 
YTQIRPKRKT KDGESLLEAL KNRGIEYVEI RSLDVNPFSP IGINKNQILL LDLFLIWCAL 

       370        380        390        400        410        420 
IDSPKIDKTD FLLTTKNWER IIYEGRKPNQ KIYINNNNET KTLIEIGQII FKDLNEIALI 

       430        440        450        460        470        480 
LDSNSNDLLY QKACKETQLF LKNPELTYSA QCLNFLMTTG IKKTGLYLAN KYHEKFINKN 

       490        500        510 
YFNLNQSVLE QEVIRSHQKK IEIEREDILS FEEYIRNK 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13108.1.
RefSeqNP_240222.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57485.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13108; BAB13108; BAB13108.
GeneID1109758.
KEGGbuc:BU407.
PATRIC21244354. VBIBucAph127364_0419.

Phylogenomic databases

eggNOGCOG2918.
HOGENOMHOG000266224.
KOK01919.
OMAWPMSMPC.
OrthoDBEOG6BKJ7H.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-407-MONOMER.
UniPathwayUPA00142; UER00209.

Family and domain databases

HAMAPMF_00578. Glu_cys_ligase.
InterProIPR007370. Glu_cys_ligase.
IPR006334. Glut_cys_ligase.
[Graphical view]
PfamPF04262. Glu_cys_ligase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01434. glu_cys_ligase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGSH1_BUCAI
AccessionPrimary (citable) accession number: P57485
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names