ID   NUSA_BUCAI              Reviewed;         496 AA.
AC   P57459;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   27-MAR-2024, entry version 132.
DE   RecName: Full=Transcription termination/antitermination protein NusA {ECO:0000255|HAMAP-Rule:MF_00945};
GN   Name=nusA {ECO:0000255|HAMAP-Rule:MF_00945}; OrderedLocusNames=BU378;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Participates in both transcription termination and
CC       antitermination. {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SUBUNIT: Monomer. Binds directly to the core enzyme of the DNA-
CC       dependent RNA polymerase and to nascent RNA. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00945}.
CC   -!- SIMILARITY: Belongs to the NusA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00945}.
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DR   EMBL; BA000003; BAB13082.1; -; Genomic_DNA.
DR   RefSeq; NP_240196.1; NC_002528.1.
DR   RefSeq; WP_009874336.1; NC_002528.1.
DR   AlphaFoldDB; P57459; -.
DR   SMR; P57459; -.
DR   STRING; 563178.BUAP5A_371; -.
DR   EnsemblBacteria; BAB13082; BAB13082; BAB13082.
DR   KEGG; buc:BU378; -.
DR   PATRIC; fig|107806.10.peg.392; -.
DR   eggNOG; COG0195; Bacteria.
DR   HOGENOM; CLU_029242_0_0_6; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006353; P:DNA-templated transcription termination; IEA:UniProtKB-UniRule.
DR   GO; GO:0031564; P:transcription antitermination; IEA:UniProtKB-UniRule.
DR   CDD; cd02134; KH-II_NusA_rpt1; 1.
DR   CDD; cd22529; KH-II_NusA_rpt2; 1.
DR   CDD; cd04455; S1_NusA; 1.
DR   Gene3D; 3.30.300.20; -; 2.
DR   Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   Gene3D; 3.30.1480.10; NusA, N-terminal domain; 1.
DR   HAMAP; MF_00945_B; NusA_B; 1.
DR   InterPro; IPR010995; DNA_repair_Rad51/TF_NusA_a-hlx.
DR   InterPro; IPR015946; KH_dom-like_a/b.
DR   InterPro; IPR025249; KH_dom_NusA-like.
DR   InterPro; IPR009019; KH_sf_prok-type.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR030842; NusA_bac.
DR   InterPro; IPR036555; NusA_N_sf.
DR   InterPro; IPR003029; S1_domain.
DR   InterPro; IPR013735; TF_NusA_N.
DR   InterPro; IPR010214; Tscrpt_termin_fac_NusA_C_rpt.
DR   InterPro; IPR010213; Tscrpt_termination_fac_NusA.
DR   NCBIfam; TIGR01953; NusA; 1.
DR   NCBIfam; TIGR01954; nusA_Cterm_rpt; 2.
DR   PANTHER; PTHR22648; TRANSCRIPTION TERMINATION FACTOR NUSA; 1.
DR   PANTHER; PTHR22648:SF0; TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA; 1.
DR   Pfam; PF14520; HHH_5; 1.
DR   Pfam; PF13184; KH_5; 1.
DR   Pfam; PF08529; NusA_N; 1.
DR   SMART; SM00316; S1; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   SUPFAM; SSF54814; Prokaryotic type KH domain (KH-domain type II); 2.
DR   SUPFAM; SSF47794; Rad51 N-terminal domain-like; 2.
DR   SUPFAM; SSF69705; Transcription factor NusA, N-terminal domain; 1.
DR   PROSITE; PS50084; KH_TYPE_1; 1.
DR   PROSITE; PS50126; S1; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; Repeat; RNA-binding; Transcription;
KW   Transcription antitermination; Transcription regulation;
KW   Transcription termination.
FT   CHAIN           1..496
FT                   /note="Transcription termination/antitermination protein
FT                   NusA"
FT                   /id="PRO_0000181962"
FT   DOMAIN          135..200
FT                   /note="S1 motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   DOMAIN          302..370
FT                   /note="KH"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
FT   REPEAT          364..414
FT                   /note="1"
FT   REPEAT          440..490
FT                   /note="2"
FT   REGION          364..490
FT                   /note="2 X 51 AA approximate repeats"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00945"
SQ   SEQUENCE   496 AA;  55995 MW;  17878203D5D4164E CRC64;
     MNKEILAVVE AVSNEKSLPR EKIFEALEMA LATATKKKHE QEIDIRVSIN RKTGGFTTFR
     RWMVVETVTQ PTREITLEAA CFDGEKVYLN DYIEEQIESV DFDRITTQTA KQVIVQKVRE
     AERAMLVEQF RKYTGQIITG IVKKINRDNI MLDLGNNAEA LILREGMLPR ENFRPGDRIR
     GILYGVYPEA RGAQLFISRS KTEMLTELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT
     NDKRIDPVGA CVGMRGARVQ AVSSELCGER IDIILWDDNP AQFVINAMAP ADVASIVVDE
     DCHTMDIAVD INNLAQAIGR NGQNVRLASQ ISGWELNVMT TEDLHSKHKE EAHTAFNFFK
     KNLNINENII KILVKEGFSS LEELAYIPFN ELLEVKNLTE DQAKKVREGA KSKLLLMESD
     KNKMIIQERK TEKELLKING MNAVLALQLA EKNIFTIEEL ADQGIDDLTD IKNLNSEQAG
     LLIMTARNIC WFSSKV
//