P57459 (NUSA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Transcription termination/antitermination protein NusA

Gene names

Name:	nusA
Ordered Locus Names:	BU378

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Participates in both transcription termination and antitermination By similarity. HAMAP-Rule MF_00945
Subunit structure	Monomer By similarity. Binds directly to the core enzyme of the DNA-dependent RNA polymerase and to nascent RNA By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NusA family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Biological process	Transcription Transcription antitermination Transcription regulation Transcription termination
Cellular component	Cytoplasm
Domain	Repeat
Ligand	RNA-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, termination Inferred from electronic annotation. Source: UniProtKB-KW transcription antitermination Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 496	496	Transcription termination/antitermination protein NusA HAMAP-Rule MF_00945		PRO_0000181962
Regions
	Domain	135 – 200	66	S1 motif
	Domain	302 – 370	69	KH
	Repeat	364 – 414	51	1 HAMAP-Rule MF_00945
	Repeat	440 – 490	51	2 HAMAP-Rule MF_00945
	Region	364 – 490	127	2 X 51 AA approximate repeats By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57459 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 17878203D5D4164E	FASTA	496	55,995
	10 20 30 40 50 60 MNKEILAVVE AVSNEKSLPR EKIFEALEMA LATATKKKHE QEIDIRVSIN RKTGGFTTFR 70 80 90 100 110 120 RWMVVETVTQ PTREITLEAA CFDGEKVYLN DYIEEQIESV DFDRITTQTA KQVIVQKVRE 130 140 150 160 170 180 AERAMLVEQF RKYTGQIITG IVKKINRDNI MLDLGNNAEA LILREGMLPR ENFRPGDRIR 190 200 210 220 230 240 GILYGVYPEA RGAQLFISRS KTEMLTELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT 250 260 270 280 290 300 NDKRIDPVGA CVGMRGARVQ AVSSELCGER IDIILWDDNP AQFVINAMAP ADVASIVVDE 310 320 330 340 350 360 DCHTMDIAVD INNLAQAIGR NGQNVRLASQ ISGWELNVMT TEDLHSKHKE EAHTAFNFFK 370 380 390 400 410 420 KNLNINENII KILVKEGFSS LEELAYIPFN ELLEVKNLTE DQAKKVREGA KSKLLLMESD 430 440 450 460 470 480 KNKMIIQERK TEKELLKING MNAVLALQLA EKNIFTIEEL ADQGIDDLTD IKNLNSEQAG 490 LLIMTARNIC WFSSKV

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13082.1.
RefSeq	NP_240196.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57459.
SMR	P57459. Positions 430-494.
ModBase	Search...
Proteomic databases
PRIDE	P57459.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13082; BAB13082; BAB13082.
GeneID	1109732.
KEGG	buc:BU378.
PATRIC	21244294. VBIBucAph127364_0392.
Phylogenomic databases
eggNOG	COG0195.
HOGENOM	HOG000006394.
KO	K02600.
OMA	ICWFGDD.
ProtClustDB	PRK09202.
Family and domain databases
Gene3D	2.40.50.140. 1 hit. 3.30.1480.10. 1 hit. 3.30.300.20. 2 hits.
HAMAP	MF_00945. NusA.
InterPro	IPR010995. DNA_repair_Rad51/TF_NusA_a-hlx. IPR004087. KH_dom. IPR015946. KH_dom-like_a/b. IPR025249. KH_dom_NusA-like. IPR004088. KH_dom_type_1. IPR009019. KH_prok-type. IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR022967. RNA-binding_domain_S1. IPR013735. TF_NusA_N. IPR010214. Tscrpt_termin_fac_NusA_C_rpt. IPR010213. Tscrpt_termination_fac_NusA. [Graphical view]
Pfam	PF13184. KH_5. 1 hit. PF08529. NusA_N. 1 hit. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00322. KH. 2 hits. SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF54814. KH_prok. 2 hits. SSF50249. Nucleic_acid_OB. 1 hit. SSF47794. Rad51_N. 2 hits. SSF69705. TF_NusA_N. 1 hit.
TIGRFAMs	TIGR01953. NusA. 1 hit. TIGR01954. nusA_Cterm_rpt. 2 hits.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUSA_BUCAI

Accession

Primary (citable) accession number: P57459

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program