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P57453 (DEAD_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent RNA helicase DeaD

EC=3.6.4.13
Alternative name(s):
Cold-shock DEAD box protein A
Gene names
Name:deaD
Synonyms:csdA
Ordered Locus Names:BU372
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

DEAD-box RNA helicase involved in various cellular processes at low temperature, including ribosome biogenesis, mRNA degradation and translation initiation By similarity. HAMAP-Rule MF_00964

Catalytic activity

ATP + H2O = ADP + phosphate. HAMAP-Rule MF_00964

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00964.

Sequence similarities

Belongs to the DEAD box helicase family. DeaD subfamily.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Ontologies

Keywords
   Biological processStress response
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
RNA-binding
   Molecular functionHelicase
Hydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processRNA catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

cellular response to cold

Inferred from electronic annotation. Source: InterPro

ribosomal large subunit assembly

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ATP-dependent RNA helicase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 601601ATP-dependent RNA helicase DeaD HAMAP-Rule MF_00964
PRO_0000055098

Regions

Domain37 – 208172Helicase ATP-binding
Domain231 – 378148Helicase C-terminal
Nucleotide binding50 – 578ATP By similarity
Motif6 – 3429Q motif HAMAP-Rule MF_00964
Motif156 – 1594DEAD box HAMAP-Rule MF_00964

Sequences

Sequence LengthMass (Da)Tools
P57453 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 886C237AC5E61279

FASTA60168,806
        10         20         30         40         50         60 
MTHIESTFSF LGLNPFIIQS LNEMGYVKPS PIQASCIPLL LEGRDVLGMA QTGSGKTAAF 

        70         80         90        100        110        120 
SLPLLHNLNI NLKAPQILVL APTRELAVQV AEAFSDFSKY MIGIHVLPLY GGQRYELQLR 

       130        140        150        160        170        180 
ALRQGPQIVV GTPGRLLDHL KRGTLNLSNL HGLVLDEADE MLRMGFIEDV ETIMAQIPKE 

       190        200        210        220        230        240 
HQTALFSATM PEAIRRISKR FMRNPKEIKI QSNITTRPDI KQSYWMVYGR KTDALIRFLE 

       250        260        270        280        290        300 
AEDFSATIIF VRTKNATLEV SEALERNGYN SAALNGDMNQ ALREQTLERL KNGRLDILIA 

       310        320        330        340        350        360 
TDVAARGLDV DRISFVINYD IPMDSESYVH RIGRTGRAGR AGRALLFVEN RERRLLRNIE 

       370        380        390        400        410        420 
RTMKQSIPEV QLPKVELLCQ RRLEQFAKKV QQQLESRDLD EYSALLDKLY STDDLDIKTL 

       430        440        450        460        470        480 
AAALLKMAQG ERPLIIKPDV IKRQSRDLLF QDDRRREDNR NSRTRRDRRD INKDAELYRI 

       490        500        510        520        530        540 
EVGRNDGVEV RHIVGAIANE GNINSRNIGN IKLFSSYSTI ELPKGMSKDL LQTFNRTRIL 

       550        560        570        580        590        600 
NKPINMKLLR DSRHYENKTT HRSIFNKDKN SNRRVSDGSF NKSNSPKKTE FKSSFFRRRN 


V 

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References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13076.1.
RefSeqNP_240190.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57453.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13076; BAB13076; BAB13076.
GeneID1109726.
KEGGbuc:BU372.
PATRIC21244282. VBIBucAph127364_0386.

Phylogenomic databases

eggNOGCOG0513.
HOGENOMHOG000268810.
KOK05592.
OMALPQGMPK.
OrthoDBEOG6GBMBM.
ProtClustDBCLSK390910.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-372-MONOMER.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
HAMAPMF_00964. DEAD_helicase_DeaD.
InterProIPR005580. DbpA_RNA-bd.
IPR028618. DEAD_helicase_DeaD.
IPR011545. DNA/RNA_helicase_DEAD/DEAH_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
[Graphical view]
PfamPF03880. DbpA. 1 hit.
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
PROSITEPS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEAD_BUCAI
AccessionPrimary (citable) accession number: P57453
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names