P57453 (DEAD_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cold-shock DEAD box protein A homolog
EC=3.6.4.13

Alternative name(s):
ATP-dependent RNA helicase DeaD homolog

Gene names

Name:	deaD
Synonyms:	csdA
Ordered Locus Names:	BU372

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Has a helix-destabilizing activity By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the DEAD box helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding RNA-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	Cold-shock DEAD box protein A homolog		PRO_0000055098
Regions
	Domain	37 – 208	172	Helicase ATP-binding
	Domain	231 – 378	148	Helicase C-terminal
	Nucleotide binding	50 – 57	8	ATP By similarity
	Motif	6 – 34	29	Q motif
	Motif	156 – 159	4	DEAD box

Sequences

	Sequence		Length	Mass (Da)
	P57453 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 886C237AC5E61279	FASTA	601	68,806
	10 20 30 40 50 60 MTHIESTFSF LGLNPFIIQS LNEMGYVKPS PIQASCIPLL LEGRDVLGMA QTGSGKTAAF 70 80 90 100 110 120 SLPLLHNLNI NLKAPQILVL APTRELAVQV AEAFSDFSKY MIGIHVLPLY GGQRYELQLR 130 140 150 160 170 180 ALRQGPQIVV GTPGRLLDHL KRGTLNLSNL HGLVLDEADE MLRMGFIEDV ETIMAQIPKE 190 200 210 220 230 240 HQTALFSATM PEAIRRISKR FMRNPKEIKI QSNITTRPDI KQSYWMVYGR KTDALIRFLE 250 260 270 280 290 300 AEDFSATIIF VRTKNATLEV SEALERNGYN SAALNGDMNQ ALREQTLERL KNGRLDILIA 310 320 330 340 350 360 TDVAARGLDV DRISFVINYD IPMDSESYVH RIGRTGRAGR AGRALLFVEN RERRLLRNIE 370 380 390 400 410 420 RTMKQSIPEV QLPKVELLCQ RRLEQFAKKV QQQLESRDLD EYSALLDKLY STDDLDIKTL 430 440 450 460 470 480 AAALLKMAQG ERPLIIKPDV IKRQSRDLLF QDDRRREDNR NSRTRRDRRD INKDAELYRI 490 500 510 520 530 540 EVGRNDGVEV RHIVGAIANE GNINSRNIGN IKLFSSYSTI ELPKGMSKDL LQTFNRTRIL 550 560 570 580 590 600 NKPINMKLLR DSRHYENKTT HRSIFNKDKN SNRRVSDGSF NKSNSPKKTE FKSSFFRRRN V

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13076.1.
RefSeq	NP_240190.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57453.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13076; BAB13076; BAB13076.
GeneID	1109726.
KEGG	buc:BU372.
PATRIC	21244282. VBIBucAph127364_0386.
Phylogenomic databases
eggNOG	COG0513.
HOGENOM	HOG000268810.
KO	K05592.
OMA	LPQGMPK.
ProtClustDB	CLSK390910.
Family and domain databases
InterPro	IPR005580. DbpA_RNA-bd. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR014001. Helicase_ATP-bd. IPR001650. Helicase_C. IPR000629. RNA-helicase_DEAD-box_CS. IPR014014. RNA_helicase_DEAD_Q_motif. [Graphical view]
Pfam	PF03880. DbpA. 1 hit. PF00270. DEAD. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00039. DEAD_ATP_HELICASE. 1 hit. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. PS51195. Q_MOTIF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DEAD_BUCAI

Accession

Primary (citable) accession number: P57453

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program