Reviewed, UniProtKB/Swiss-Prot P57450 (PYRB_BUCAI)

Names and origin

Protein names

Recommended name:
    Aspartate carbamoyltransferase catalytic chain
    EC=2.1.3.2
Alternative name(s):
    Aspartate transcarbamylase
      Short name=ATCase

Gene names

Name:	pyrB
Ordered Locus Names:	BU369

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 2/6.
Subunit structure	Contains six catalytic and six regulatory chains By similarity.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Aspartate carbamoyltransferase catalytic chain		PRO_0000113110

Sequences

	Sequence		Length	Mass (Da)
	P57450-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: B02131513FA8D388	FASTA	310	35,553
	10 20 30 40 50 60 MRNSLYKKNI ISINDLQRNE LELVLNKSAM LKKTPQPNLL KNKVIASCFF EASTRTRLSF 70 80 90 100 110 120 ETAIYRLGAS IVGFSDGNNI SLEKKGETLT DTISVISSYV DAIIIRHPQE GSARLAAEFS 130 140 150 160 170 180 NKKPIFNAGD GANQHPTQTL LDLFTIQETQ NRLTQLNIAI VGDLKYGRTV HSLTQALAKF 190 200 210 220 230 240 KHNKFYFISP DALKMPNYIN NMLDKKEIYW KRHNNIEEII SEIDILYMTR IQKERLDSTE 250 260 270 280 290 300 YANAKSKFVL RAAILKNARN NMKILHPLPR IDEIDRDVDY TPYAWYFKQA ANGIYARQAI 310 LSLVLIEKHL

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13073.1.
RefSeq	NP_240187.1.
3D structure databases
HSSP	HSSP built from PDB template 3CSU based on UniProtKB P00479.
SMR	P57450. Positions 3-305.
ModBase	Search...
Genome annotation databases
GeneID	1109723.
GenomeReviews	Gene locus BU369 in contig BA000003_GR.
KEGG	buc:BU369.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57450.
Enzyme and pathway databases
BioCyc	BSP107806:BU369-MON.
Family and domain databases
HAMAP	MF_00001. [Tree]
InterPro	IPR006130. Asp/Orn_carbamoyltranf. IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002082. Aspartate_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
TIGRFAMs	TIGR00670. asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BUCAI

Accession

Primary (citable) accession number: P57450

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)