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Protein

Ornithine carbamoyltransferase

Gene

argI

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argI)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10Carbamoyl phosphateBy similarity1
Binding sitei72Carbamoyl phosphateBy similarity1
Binding sitei107Carbamoyl phosphateBy similarity1
Binding sitei168OrnithineBy similarity1
Binding sitei232OrnithineBy similarity1
Binding sitei302Carbamoyl phosphateBy similarity1
Binding sitei320Carbamoyl phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argI
Ordered Locus Names:BU368
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001128971 – 338Ornithine carbamoyltransferaseAdd BLAST338

Interactioni

Protein-protein interaction databases

STRINGi107806.BU368.

Structurei

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei31Important for structural integrityBy similarity1
Sitei147Important for structural integrityBy similarity1

3D structure databases

ProteinModelPortaliP57449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni56 – 60Carbamoyl phosphate bindingBy similarity5
Regioni134 – 137Carbamoyl phosphate bindingBy similarity4
Regioni236 – 237Ornithine bindingBy similarity2
Regioni273 – 276Carbamoyl phosphate bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiSACWPEA.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLYQRDCL RLLDFTSLEL QNIITLAQKL KQYKKNNKEI QLLKKKNIAL
60 70 80 90 100
IFEKESTRTR CSFEVAAFDQ GAHVTYLGPG STHLGTKESI EDTAKILGRL
110 120 130 140 150
YDGIQYRGHH HSTIEILAKN SKVPVWNGLT EKFHPTQLLA DLLTIKEIFP
160 170 180 190 200
ERKFYEIKCA YVGDAHNNMG NSLLEAASLV GLDLRLVAPK ECWPEKNIFK
210 220 230 240 250
FCKEQMKNKK GNIICTENIN EGVKNVDFIY TDVWVSMGES QEVWKKRIEL
260 270 280 290 300
LSSYQVNSLM LKITNNPQVK VLHCLPALHD QKTCTVKSIL KKYGFKNGME
310 320 330
ITDEVFQKNQ KIIFEQAENR LHTIKAILVS SLLKTIKF
Length:338
Mass (Da):38,865
Last modified:December 1, 2000 - v1
Checksum:iE6CCAEE0584668E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13072.1.
RefSeqiNP_240186.1. NC_002528.1.
WP_010896090.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13072; BAB13072; BAB13072.
GeneIDi1109722.
KEGGibuc:BU368.
PATRICi21244274. VBIBucAph127364_0382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13072.1.
RefSeqiNP_240186.1. NC_002528.1.
WP_010896090.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU368.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13072; BAB13072; BAB13072.
GeneIDi1109722.
KEGGibuc:BU368.
PATRICi21244274. VBIBucAph127364_0382.

Phylogenomic databases

eggNOGiENOG4105DBV. Bacteria.
COG0078. LUCA.
HOGENOMiHOG000022686.
KOiK00611.
OMAiSACWPEA.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase. 1 hit.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. OTCase_ArgI.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_BUCAI
AccessioniPrimary (citable) accession number: P57449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.