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Protein

Ornithine carbamoyltransferase

Gene

argI

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline.By similarity

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Carbamoyl phosphateBy similarity
Sitei31 – 311Important for structural integrityBy similarity
Binding sitei72 – 721Carbamoyl phosphateBy similarity
Binding sitei107 – 1071Carbamoyl phosphateBy similarity
Sitei147 – 1471Important for structural integrityBy similarity
Binding sitei168 – 1681OrnithineBy similarity
Binding sitei232 – 2321OrnithineBy similarity
Binding sitei302 – 3021Carbamoyl phosphateBy similarity
Binding sitei320 – 3201Carbamoyl phosphateBy similarity

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. ornithine carbamoyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. arginine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-368-MONOMER.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase (EC:2.1.3.3)
Short name:
OTCase
Gene namesi
Name:argI
Ordered Locus Names:BU368
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338Ornithine carbamoyltransferasePRO_0000112897Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57449.
SMRiP57449. Positions 3-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni56 – 605Carbamoyl phosphate bindingBy similarity
Regioni134 – 1374Carbamoyl phosphate bindingBy similarity
Regioni236 – 2372Ornithine bindingBy similarity
Regioni273 – 2764Carbamoyl phosphate bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
KOiK00611.
OMAiYLGKGDI.
OrthoDBiEOG690MGV.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLYQRDCL RLLDFTSLEL QNIITLAQKL KQYKKNNKEI QLLKKKNIAL
60 70 80 90 100
IFEKESTRTR CSFEVAAFDQ GAHVTYLGPG STHLGTKESI EDTAKILGRL
110 120 130 140 150
YDGIQYRGHH HSTIEILAKN SKVPVWNGLT EKFHPTQLLA DLLTIKEIFP
160 170 180 190 200
ERKFYEIKCA YVGDAHNNMG NSLLEAASLV GLDLRLVAPK ECWPEKNIFK
210 220 230 240 250
FCKEQMKNKK GNIICTENIN EGVKNVDFIY TDVWVSMGES QEVWKKRIEL
260 270 280 290 300
LSSYQVNSLM LKITNNPQVK VLHCLPALHD QKTCTVKSIL KKYGFKNGME
310 320 330
ITDEVFQKNQ KIIFEQAENR LHTIKAILVS SLLKTIKF
Length:338
Mass (Da):38,865
Last modified:December 1, 2000 - v1
Checksum:iE6CCAEE0584668E5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13072.1.
RefSeqiNP_240186.1. NC_002528.1.
WP_010896090.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13072; BAB13072; BAB13072.
GeneIDi1109722.
KEGGibuc:BU368.
PATRICi21244274. VBIBucAph127364_0382.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13072.1.
RefSeqiNP_240186.1. NC_002528.1.
WP_010896090.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57449.
SMRiP57449. Positions 3-332.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13072; BAB13072; BAB13072.
GeneIDi1109722.
KEGGibuc:BU368.
PATRICi21244274. VBIBucAph127364_0382.

Phylogenomic databases

eggNOGiCOG0078.
HOGENOMiHOG000022686.
KOiK00611.
OMAiYLGKGDI.
OrthoDBiEOG690MGV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
BioCyciBAPH107806:GBZJ-368-MONOMER.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
HAMAPiMF_01109. OTCase.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiOTC_BUCAI
AccessioniPrimary (citable) accession number: P57449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 4, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.