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P57449 (OTC_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ornithine carbamoyltransferase

Short name=OTCase
EC=2.1.3.3
Gene names
Name:argI
Ordered Locus Names:BU368
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reversibly catalyzes the transfer of the carbamoyl group from carbamoyl phosphate (CP) to the N(epsilon) atom of ornithine (ORN) to produce L-citrulline By similarity. HAMAP-Rule MF_01109

Catalytic activity

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP-Rule MF_01109

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP-Rule MF_01109

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01109.

Sequence similarities

Belongs to the ATCase/OTCase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionamino acid binding

Inferred from electronic annotation. Source: InterPro

ornithine carbamoyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Ornithine carbamoyltransferase HAMAP-Rule MF_01109
PRO_0000112897

Regions

Region56 – 605Carbamoyl phosphate binding By similarity
Region134 – 1374Carbamoyl phosphate binding By similarity
Region236 – 2372Ornithine binding By similarity
Region273 – 2764Carbamoyl phosphate binding By similarity

Sites

Binding site101Carbamoyl phosphate By similarity
Binding site721Carbamoyl phosphate By similarity
Binding site1071Carbamoyl phosphate By similarity
Binding site1681Ornithine By similarity
Binding site2321Ornithine By similarity
Binding site3021Carbamoyl phosphate By similarity
Binding site3201Carbamoyl phosphate By similarity
Site311Important for structural integrity By similarity
Site1471Important for structural integrity By similarity

Sequences

Sequence LengthMass (Da)Tools
P57449 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: E6CCAEE0584668E5

FASTA33838,865
        10         20         30         40         50         60 
MNNLYQRDCL RLLDFTSLEL QNIITLAQKL KQYKKNNKEI QLLKKKNIAL IFEKESTRTR 

        70         80         90        100        110        120 
CSFEVAAFDQ GAHVTYLGPG STHLGTKESI EDTAKILGRL YDGIQYRGHH HSTIEILAKN 

       130        140        150        160        170        180 
SKVPVWNGLT EKFHPTQLLA DLLTIKEIFP ERKFYEIKCA YVGDAHNNMG NSLLEAASLV 

       190        200        210        220        230        240 
GLDLRLVAPK ECWPEKNIFK FCKEQMKNKK GNIICTENIN EGVKNVDFIY TDVWVSMGES 

       250        260        270        280        290        300 
QEVWKKRIEL LSSYQVNSLM LKITNNPQVK VLHCLPALHD QKTCTVKSIL KKYGFKNGME 

       310        320        330 
ITDEVFQKNQ KIIFEQAENR LHTIKAILVS SLLKTIKF 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13072.1.
RefSeqNP_240186.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57449.
SMRP57449. Positions 3-332.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13072; BAB13072; BAB13072.
GeneID1109722.
KEGGbuc:BU368.
PATRIC21244274. VBIBucAph127364_0382.

Phylogenomic databases

eggNOGCOG0078.
HOGENOMHOG000022686.
KOK00611.
OMAYQRHFLR.
OrthoDBEOG690MGV.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-368-MONOMER.
UniPathwayUPA00068; UER00112.

Family and domain databases

Gene3D3.40.50.1370. 2 hits.
HAMAPMF_01109. OTCase.
InterProIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
IPR024904. Orn_carbamltrans.
[Graphical view]
PfamPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMSSF53671. SSF53671. 1 hit.
TIGRFAMsTIGR00658. orni_carb_tr. 1 hit.
PROSITEPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOTC_BUCAI
AccessionPrimary (citable) accession number: P57449
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names