P57448 (AMPA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	BU367

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Cytosol aminopeptidase HAMAP MF_00181		PRO_0000165730
Sites
	Active site	279	1	Potential
	Active site	353	1	Potential
	Metal binding	267	1	Manganese 2 By similarity
	Metal binding	272	1	Manganese 1 By similarity
	Metal binding	272	1	Manganese 2 By similarity
	Metal binding	290	1	Manganese 2 By similarity
	Metal binding	349	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 1 By similarity
	Metal binding	351	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57448 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: D4FDB26BBE24A957	FASTA	499	55,268
	10 20 30 40 50 60 MNFFIKSCFL DKEKTDCIVV SVFELSELSD SAIYLDKCSN GHITSLIKLG DIQGKIGDTL 70 80 90 100 110 120 MLYKVPKILS KRILLVGCGK KDEINIIRFK KILKNTIHAI KKKSITNIVY SFSNINIDNI 130 140 150 160 170 180 YWMIRRMVLS LKESLYETIK INNINIKNTN IHSITLNIIK KNDLFIAKTA LKHALAIDHA 190 200 210 220 230 240 ITSTKNLSNL PPNICNPLYL SYKAQELSKK YENNIVVEII DIKKMKELGM NAYIAVGNGS 250 260 270 280 290 300 KNKPFMSVIK YSGNNIVNKK IIAFVGKGLT FDSGGISIKP ALHMHEMKYD MCGAAAVYGT 310 320 330 340 350 360 LIMAAELQLP LTVIGILSGC ENMVGSHSFR PGDVLTTMSG QTVEILNTDA EGRLVLCDSL 370 380 390 400 410 420 TYLERFSPDI VIDVATLTGA CVTALGESVS GLFSNNEELS NQLLHASQET DDKIWSLPLF 430 440 450 460 470 480 SEYHKELNSD IADFSNIGRG KAGAITAACF LSKFTKKYNW AHLDIAGTAW KSGKKSGATG 490 RPVELLCQFL LNQSNYIYN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13071.1.
RefSeq	NP_240185.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57448.
SMR	P57448. Positions 1-497.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003172; EBBUCP00000002948; EBBUCG00000003172.
GeneID	1109721.
GenomeReviews	Gene locus BU367 in contig BA000003_GR.
KEGG	buc:BU367.
PATRIC	21244272. VBIBucAph127364_0381.
Phylogenomic databases
GeneTree	EBGT00050000008030.
HOGENOM	HBG742580.
OMA	MPANICT.
PhylomeDB	P57448.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	BSP107806:BU367-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BUCAI

Accession

Primary (citable) accession number: P57448

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program