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P57443 (PYRD_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone)
EC=1.3.5.2
Alternative name(s):
DHOdehase
Short name=DHOD
Short name=DHODase
Dihydroorotate oxidase
Gene names
Name:pyrD
Ordered Locus Names:BU362
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length337 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP MF_00225

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP MF_00225

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225

Subunit structure

Monomer By similarity. HAMAP MF_00225

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Sequence caution

The sequence BAB13066.1 differs from that shown. Reason: Frameshift at position 46.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFMN
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological process'de novo' pyrimidine base biosynthetic process

Inferred from electronic annotation. Source: InterPro

UMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 337337Dihydroorotate dehydrogenase (quinone) HAMAP MF_00225
PRO_0000148426

Regions

Nucleotide binding63 – 675FMN By similarity
Nucleotide binding319 – 3202FMN By similarity
Region112 – 1165Substrate binding By similarity
Region247 – 2482Substrate binding By similarity

Sites

Active site1761Nucleophile By similarity
Binding site671Substrate By similarity
Binding site871FMN; via amide nitrogen By similarity
Binding site1401FMN By similarity
Binding site1731FMN By similarity
Binding site1731Substrate By similarity
Binding site1781Substrate By similarity
Binding site2181FMN By similarity
Binding site2461FMN; via carbonyl oxygen By similarity
Binding site2691FMN; via amide nitrogen By similarity
Binding site2981FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
P57443 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 5CC459EB4E6AB772

FASTA33737,955
        10         20         30         40         50         60 
MFYYFIRKLL FLLEPEKAHF LTLKYLNIKN IQFFDFFFYK TIILSKKIKC MGLIFDNKLG 

        70         80         90        100        110        120 
TAAGIDKNGE YIDALSKLGF GFIEVGTVTP LPQVGNPKPR MFRIVSMEGI INRMGFNNLG 

       130        140        150        160        170        180 
IDNLIRNIKK SNFKGIIGVN IGKNKSTSLE NSVDDYLICI EKIYCYAGYI AINISSPNTT 

       190        200        210        220        230        240 
NLRNLQYGIL FKKLLYKIKK KQKELHKKYL KYVPIAIKIS PDLSIKELVD ISKQLIRYKI 

       250        260        270        280        290        300 
DAVIATNTTL DHSSLFGLKN SSEQGGLSGL PLQKKSTNTI SILSKSLQKK IPIIGVGGIN 

       310        320        330 
SINSAKEKIE SGATLIQIYS GLVYHGPKLI KKIINHI

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB13066.1. Frameshift.
RefSeqNP_240180.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57443.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBUCT00000002786; EBBUCP00000002562; EBBUCG00000002786.
GeneID1110058.
GenomeReviewsGene locus BU362 in contig BA000003_GR.
KEGGbuc:BU362.
PATRIC21244262. VBIBucAph127364_0376.

Phylogenomic databases

GeneTreeEBGT00050000007915.
HOGENOMHBG351027.
OMASYVTVNI.
PhylomeDBP57443.
ProtClustDBPRK05286.

Enzyme and pathway databases

BioCycBSP107806:BU362-MONOMER.

Family and domain databases

HAMAPMF_00225. DHO_dh_type2.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK00226.
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. PyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRD_BUCAI
AccessionPrimary (citable) accession number: P57443
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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