Reviewed, UniProtKB/Swiss-Prot P57443 (PYRD_BUCAI)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.3.1
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	BU362

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + O(2) = orotate + H(2)O(2).
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane proteinBy similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.
Sequence caution	The sequence BAB13066.1 differs from that shown. Reason: Frameshift at position 46.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Dihydroorotate dehydrogenase		PRO_0000148426
Sites
	Active site	176	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57443-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 5CC459EB4E6AB772	FASTA	337	37,955
	10 20 30 40 50 60 MFYYFIRKLL FLLEPEKAHF LTLKYLNIKN IQFFDFFFYK TIILSKKIKC MGLIFDNKLG 70 80 90 100 110 120 TAAGIDKNGE YIDALSKLGF GFIEVGTVTP LPQVGNPKPR MFRIVSMEGI INRMGFNNLG 130 140 150 160 170 180 IDNLIRNIKK SNFKGIIGVN IGKNKSTSLE NSVDDYLICI EKIYCYAGYI AINISSPNTT 190 200 210 220 230 240 NLRNLQYGIL FKKLLYKIKK KQKELHKKYL KYVPIAIKIS PDLSIKELVD ISKQLIRYKI 250 260 270 280 290 300 DAVIATNTTL DHSSLFGLKN SSEQGGLSGL PLQKKSTNTI SILSKSLQKK IPIIGVGGIN 310 320 330 SINSAKEKIE SGATLIQIYS GLVYHGPKLI KKIINHI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13066.1. Frameshift.
RefSeq	NP_240180.1.
3D structure databases
HSSP	HSSP built from PDB template 1F76 based on UniProtKB P05021.
ModBase	Search...
Genome annotation databases
GeneID	1110058.
GenomeReviews	Gene locus BU362 in contig BA000003_GR.
KEGG	buc:BU362.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57443.
Enzyme and pathway databases
BioCyc	BSP107806:BU362-MON.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. DHO_DHase_1_2. IPR005719. DHO_DHase_2. IPR001295. Dihydroorotate_DHase_core. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
LinkHub	P57443.

Entry information

Entry name

PYRD_BUCAI

Accession

Primary (citable) accession number: P57443

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)