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P57441 (SYN_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Asparagine--tRNA ligase
EC=6.1.1.22
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS
Gene names
Name:asnS
Ordered Locus Names:BU360
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534

Subunit structure

Homodimer By similarity. HAMAP MF_00534

Subcellular location

Cytoplasm HAMAP MF_00534.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processasparaginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

asparagine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Asparagine--tRNA ligase HAMAP MF_00534
PRO_0000176397

Sequences

Sequence LengthMass (Da)Tools
P57441 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: D97B360FD0AF26D0

FASTA46653,975
        10         20         30         40         50         60 
MNRVSISDIY KDDIIVNSLI TVCGWVRNRR SSKSGFSFIT IYDGSCFNSI QVIANNTLPN 

        70         80         90        100        110        120 
YYKEILHLTT GCSVMLSGKL ILSIGDKQKY EIQLKKIKVL GWVENPETYP ISSKKHSTEY 

       130        140        150        160        170        180 
LREVAHLRSR TNLIGAIARI RNYILHSLHH FFYKEKYYWV PTPIITSLNT EGAGEMFRVS 

       190        200        210        220        230        240 
TLDMENIPKN LDSSVDFKKD FFGKESFLTV SGQLNLETYA CSLSKVYTFG PTFRAENSNT 

       250        260        270        280        290        300 
GRHLAEFWML EVESAFTDLN DISDFAECML KYVCKSLLKK CITDINFLEN YTNSNIVDRL 

       310        320        330        340        350        360 
EKFLLVDFVR IDYVEVINIL LDSKIKFNNS VFLGIDLSSE HERFLVENYF KIPVIVKNYP 

       370        380        390        400        410        420 
KELKAFYMRL NNDKKTVAAI DILVPNVGEL IGGSQREERI AILDERLLEL GLKKEDYWWY 

       430        440        450        460 
RDLRRYGTVP HSGFGMGFER LISYFTGITN IRDLIPFPRT VNNAYF

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB13064.1.
RefSeqNP_240178.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57441.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBBUCT00000002898; EBBUCP00000002674; EBBUCG00000002898.
GeneID1110056.
GenomeReviewsGene locus BU360 in contig BA000003_GR.
KEGGbuc:BU360.
PATRIC21244256. VBIBucAph127364_0373.

Phylogenomic databases

GeneTreeEBGT00050000007970.
HOGENOMHBG745843.
OMAAIHRFFH.
PhylomeDBP57441.
ProtClustDBPRK03932.

Enzyme and pathway databases

BioCycBSP107806:BU360-MONOMER.

Family and domain databases

HAMAPMF_00534. Asn_tRNA_synth.
[Tree]
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-synth_IIb.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004365. NA-bd_OB_tRNA-helicase.
[Graphical view]
Gene3DG3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01893.
PANTHERPTHR22594. aa-tRNA-synt_II. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMsTIGR00457. AsnS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYN_BUCAI
AccessionPrimary (citable) accession number: P57441
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents