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P57437

- PTGCB_BUCAI

UniProt

P57437 - PTGCB_BUCAI

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Protein

PTS system glucose-specific EIICB component

Gene

ptsG

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport (By similarity).By similarity

Catalytic activityi

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei421 – 4211Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotation

GO - Molecular functioni

  1. glucose transmembrane transporter activity Source: InterPro
  2. kinase activity Source: UniProtKB-KW
  3. protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICB component
Alternative name(s):
EIICB-Glc
Short name:
EII-Glc
Including the following 2 domains:
Glucose permease IIC component
Alternative name(s):
PTS system glucose-specific EIIC component
Glucose-specific phosphotransferase enzyme IIB component (EC:2.7.1.69)
Alternative name(s):
PTS system glucose-specific EIIB component
Gene namesi
Name:ptsG
Ordered Locus Names:BU356
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei15 – 3521HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei51 – 7121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei76 – 9621HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei112 – 13221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei152 – 17221HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei191 – 21121HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei250 – 27021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei280 – 30021HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei304 – 32421HelicalPROSITE-ProRule annotationAdd
BLAST
Transmembranei357 – 37721HelicalPROSITE-ProRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477PTS system glucose-specific EIICB componentPRO_0000186525Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57437.
SMRiP57437. Positions 401-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 388388PTS EIIC type-1PROSITE-ProRule annotationAdd
BLAST
Domaini399 – 47779PTS EIIB type-1PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.
The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similaritiesi

Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1263.
HOGENOMiHOG000250993.
KOiK02778.
K02779.
OMAiMQIGEFT.
OrthoDBiEOG6FFS9V.

Family and domain databases

Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57437-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFKNVFANLQ KVGKSLMLPV SVLPIAGILL GIGSAHFNFL PDILSQIMAQ
60 70 80 90 100
TGGSVFSNMP LIFAIGVALG FTNNDGVAAL AAVVSYGILI QTLTAVEPIV
110 120 130 140 150
LHTTIEVIKN KHLSDTGILG GIIAGAISAY MFNKFYRIQL PEYLGFFAGK
160 170 180 190 200
RFVPIISGLS AILIGVILSL IWPPIGHGIQ IFSKWAAYQN PILAFALYGL
210 220 230 240 250
VERALVPFGL HHIWNVPFQM QIGEYTNSIG QVFHGDIARY MAGDSTAGNL
260 270 280 290 300
SGGFIFKMYG LPGAALAIWH TSKKENKTKI GSIMISAALT AFLTGITEPI
310 320 330 340 350
EFSFIIVAPV LYVIHAILAG LSFPLCIFLD MRAGTSFSHG FIDFIVLSGN
360 370 380 390 400
SHHILLFPII GILYGLLYYI LFYLFIINFN LDTPGRENIK NNILEKDNNE
410 420 430 440 450
IAPYIITALG GKNNIKNLDA CITRLRITVS DISKVKQKDL KNIGAAGIII
460 470
SGSGIQVVFG TRSENIKTAM DECIKNI
Length:477
Mass (Da):51,727
Last modified:December 1, 2000 - v1
Checksum:i5204C68E94477C5F
GO

Sequence cautioni

The sequence BAB13060.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13060.1. Different initiation.
RefSeqiNP_240174.2. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13060; BAB13060; BAB13060.
GeneIDi1110052.
KEGGibuc:BU356.
PATRICi21244248. VBIBucAph127364_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13060.1 . Different initiation.
RefSeqi NP_240174.2. NC_002528.1.

3D structure databases

ProteinModelPortali P57437.
SMRi P57437. Positions 401-476.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13060 ; BAB13060 ; BAB13060 .
GeneIDi 1110052.
KEGGi buc:BU356.
PATRICi 21244248. VBIBucAph127364_0369.

Phylogenomic databases

eggNOGi COG1263.
HOGENOMi HOG000250993.
KOi K02778.
K02779.
OMAi MQIGEFT.
OrthoDBi EOG6FFS9V.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-356-MONOMER.

Family and domain databases

Gene3Di 3.30.1360.60. 1 hit.
InterProi IPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view ]
Pfami PF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view ]
SUPFAMi SSF55604. SSF55604. 1 hit.
TIGRFAMsi TIGR00826. EIIB_glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEi PS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiPTGCB_BUCAI
AccessioniPrimary (citable) accession number: P57437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3