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P57437 (PTGCB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system glucose-specific EIICB component
Alternative name(s):
EIICB-Glc
Short name=EII-Glc

Including the following 2 domains:

  1. Glucose permease IIC component
    Alternative name(s):
    PTS system glucose-specific EIIC component
  2. Glucose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system glucose-specific EIIB component
Gene names
Name:ptsG
Ordered Locus Names:BU356
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera ›

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-1 domain.

Contains 1 PTS EIIC type-1 domain.

Sequence caution

The sequence BAB13060.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477PTS system glucose-specific EIICB component
PRO_0000186525

Regions

Transmembrane15 – 3521Helical; Potential
Transmembrane51 – 7121Helical; Potential
Transmembrane76 – 9621Helical; Potential
Transmembrane112 – 13221Helical; Potential
Transmembrane152 – 17221Helical; Potential
Transmembrane191 – 21121Helical; Potential
Transmembrane250 – 27021Helical; Potential
Transmembrane280 – 30021Helical; Potential
Transmembrane304 – 32421Helical; Potential
Transmembrane357 – 37721Helical; Potential
Domain1 – 388388PTS EIIC type-1
Domain399 – 47779PTS EIIB type-1

Sites

Active site4211Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P57437 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 5204C68E94477C5F

FASTA47751,727
        10         20         30         40         50         60 
MFKNVFANLQ KVGKSLMLPV SVLPIAGILL GIGSAHFNFL PDILSQIMAQ TGGSVFSNMP 

        70         80         90        100        110        120 
LIFAIGVALG FTNNDGVAAL AAVVSYGILI QTLTAVEPIV LHTTIEVIKN KHLSDTGILG 

       130        140        150        160        170        180 
GIIAGAISAY MFNKFYRIQL PEYLGFFAGK RFVPIISGLS AILIGVILSL IWPPIGHGIQ 

       190        200        210        220        230        240 
IFSKWAAYQN PILAFALYGL VERALVPFGL HHIWNVPFQM QIGEYTNSIG QVFHGDIARY 

       250        260        270        280        290        300 
MAGDSTAGNL SGGFIFKMYG LPGAALAIWH TSKKENKTKI GSIMISAALT AFLTGITEPI 

       310        320        330        340        350        360 
EFSFIIVAPV LYVIHAILAG LSFPLCIFLD MRAGTSFSHG FIDFIVLSGN SHHILLFPII 

       370        380        390        400        410        420 
GILYGLLYYI LFYLFIINFN LDTPGRENIK NNILEKDNNE IAPYIITALG GKNNIKNLDA 

       430        440        450        460        470 
CITRLRITVS DISKVKQKDL KNIGAAGIII SGSGIQVVFG TRSENIKTAM DECIKNI

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB13060.1. Different initiation.
RefSeqNP_240174.2. NC_002528.1.

3D structure databases

ProteinModelPortalP57437.
SMRP57437. Positions 401-476.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13060; BAB13060; BAB13060.
GeneID1110052.
KEGGbuc:BU356.
PATRIC21244248. VBIBucAph127364_0369.

Phylogenomic databases

eggNOGCOG1263.
HOGENOMHOG000250993.
KOK02778.
K02779.
OMAPFLANDS.
ProtClustDBPRK11089.

Family and domain databases

Gene3D3.30.1360.60. 1 hit.
InterProIPR018113. PTrfase_EIIB/Cys_phosph_CS.
IPR001996. PTS_EIIB_1.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMSSF55604. PTS_EIIB. 1 hit.
TIGRFAMsTIGR00826. EIIB_glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTGCB_BUCAI
AccessionPrimary (citable) accession number: P57437
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

SIMILARITY comments

Index of protein domains and families

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