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Reviewed, UniProtKB/Swiss-Prot P57437 (PTGCB_BUCAI)

Last modified November 3, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    PTS system glucose-specific EIICB component
Alternative name(s):
    EIICB-Glc
      Short name=EII-Glc
Including the following 2 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
Gene names
Name: ptsG
Ordered Locus Names: BU356
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]
Taxonomic identifier118099 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-1 domain.

Contains 1 PTS EIIC type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477PTS system glucose-specific EIICB component
PRO_0000186525

Regions

Transmembrane15 – 3521 Potential
Transmembrane51 – 7121 Potential
Transmembrane76 – 9621 Potential
Transmembrane112 – 13221 Potential
Transmembrane152 – 17221 Potential
Transmembrane191 – 21121 Potential
Transmembrane250 – 27021 Potential
Transmembrane280 – 30021 Potential
Transmembrane304 – 32421 Potential
Transmembrane357 – 37721 Potential
Domain1 – 388388PTS EIIC type-1
Domain399 – 47779PTS EIIB type-1

Sites

Active site4211Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P57437-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 5204C68E94477C5F

FASTA47751,727
        10         20         30         40         50         60 
MFKNVFANLQ KVGKSLMLPV SVLPIAGILL GIGSAHFNFL PDILSQIMAQ TGGSVFSNMP 

        70         80         90        100        110        120 
LIFAIGVALG FTNNDGVAAL AAVVSYGILI QTLTAVEPIV LHTTIEVIKN KHLSDTGILG 

       130        140        150        160        170        180 
GIIAGAISAY MFNKFYRIQL PEYLGFFAGK RFVPIISGLS AILIGVILSL IWPPIGHGIQ 

       190        200        210        220        230        240 
IFSKWAAYQN PILAFALYGL VERALVPFGL HHIWNVPFQM QIGEYTNSIG QVFHGDIARY 

       250        260        270        280        290        300 
MAGDSTAGNL SGGFIFKMYG LPGAALAIWH TSKKENKTKI GSIMISAALT AFLTGITEPI 

       310        320        330        340        350        360 
EFSFIIVAPV LYVIHAILAG LSFPLCIFLD MRAGTSFSHG FIDFIVLSGN SHHILLFPII 

       370        380        390        400        410        420 
GILYGLLYYI LFYLFIINFN LDTPGRENIK NNILEKDNNE IAPYIITALG GKNNIKNLDA 

       430        440        450        460        470 
CITRLRITVS DISKVKQKDL KNIGAAGIII SGSGIQVVFG TRSENIKTAM DECIKNI 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tokyo 1998.

Cross-references

Sequence databases

BA000003 Genomic DNA. Translation: BAB13060.1. Different initiation.
RefSeqNP_240174.2.

3D structure databases

HSSPHSSP built from PDB template 1IBA based on UniProtKB P05053.
ModBaseSearch...

Genome annotation databases

GeneID1110052.
GenomeReviewsGene locus BU356 in contig BA000003_GR.
KEGGbuc:BU356.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP57437.
OMANNDGVAG.

Enzyme and pathway databases

BioCycBSP107806:BU356-MON.

Family and domain databases

InterProIPR018113. PTrfase_EIIB/Cys_phosph_CS.
IPR001996. PTS_EIIB.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR011535. PTS_Glc-like_IIB_component.
IPR011299. PTS_IIBC_glc.
[Graphical view]
Gene3DG3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
PfamPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
TIGRFAMsTIGR00826. EIIB_glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTGCB_BUCAI
AccessionPrimary (citable) accession number: P57437
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents