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Protein

PTS system glucose-specific EIICB component

Gene

ptsG

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II complex composed of PtsG and Crr is involved in glucose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei421Phosphocysteine intermediate; for EIIB activityPROSITE-ProRule annotationBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system glucose-specific EIICB componentBy similarity
Alternative name(s):
EIICB-GlcBy similarity
Short name:
EII-GlcBy similarity
Including the following 2 domains:
Glucose permease IIC componentBy similarity
Alternative name(s):
PTS system glucose-specific EIIC componentBy similarity
Glucose-specific phosphotransferase enzyme IIB componentBy similarity (EC:2.7.1.199By similarity)
Alternative name(s):
PTS system glucose-specific EIIB componentBy similarity
Gene namesi
Name:ptsG
Ordered Locus Names:BU356
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei15 – 35HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei51 – 71HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei76 – 96HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei112 – 132HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei152 – 172HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei191 – 211HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei250 – 270HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei280 – 300HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei304 – 324HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei357 – 377HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865251 – 477PTS system glucose-specific EIICB componentAdd BLAST477

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei421PhosphocysteineBy similarity1

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi107806.BU356.

Structurei

3D structure databases

ProteinModelPortaliP57437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 388PTS EIIC type-1PROSITE-ProRule annotationAdd BLAST388
Domaini399 – 477PTS EIIB type-1PROSITE-ProRule annotationAdd BLAST79

Domaini

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.PROSITE-ProRule annotation
The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIB type-1 domain.PROSITE-ProRule annotation
Contains 1 PTS EIIC type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000250993.
KOiK02778.
K02779.
OMAiMQIGEFT.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKNVFANLQ KVGKSLMLPV SVLPIAGILL GIGSAHFNFL PDILSQIMAQ
60 70 80 90 100
TGGSVFSNMP LIFAIGVALG FTNNDGVAAL AAVVSYGILI QTLTAVEPIV
110 120 130 140 150
LHTTIEVIKN KHLSDTGILG GIIAGAISAY MFNKFYRIQL PEYLGFFAGK
160 170 180 190 200
RFVPIISGLS AILIGVILSL IWPPIGHGIQ IFSKWAAYQN PILAFALYGL
210 220 230 240 250
VERALVPFGL HHIWNVPFQM QIGEYTNSIG QVFHGDIARY MAGDSTAGNL
260 270 280 290 300
SGGFIFKMYG LPGAALAIWH TSKKENKTKI GSIMISAALT AFLTGITEPI
310 320 330 340 350
EFSFIIVAPV LYVIHAILAG LSFPLCIFLD MRAGTSFSHG FIDFIVLSGN
360 370 380 390 400
SHHILLFPII GILYGLLYYI LFYLFIINFN LDTPGRENIK NNILEKDNNE
410 420 430 440 450
IAPYIITALG GKNNIKNLDA CITRLRITVS DISKVKQKDL KNIGAAGIII
460 470
SGSGIQVVFG TRSENIKTAM DECIKNI
Length:477
Mass (Da):51,727
Last modified:December 1, 2000 - v1
Checksum:i5204C68E94477C5F
GO

Sequence cautioni

The sequence BAB13060 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13060.1. Different initiation.
RefSeqiNP_240174.2. NC_002528.1.
WP_009874312.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13060; BAB13060; BAB13060.
GeneIDi1110052.
KEGGibuc:BU356.
PATRICi21244248. VBIBucAph127364_0369.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13060.1. Different initiation.
RefSeqiNP_240174.2. NC_002528.1.
WP_009874312.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57437.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU356.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13060; BAB13060; BAB13060.
GeneIDi1110052.
KEGGibuc:BU356.
PATRICi21244248. VBIBucAph127364_0369.

Phylogenomic databases

eggNOGiENOG4105CI1. Bacteria.
COG1263. LUCA.
COG1264. LUCA.
HOGENOMiHOG000250993.
KOiK02778.
K02779.
OMAiMQIGEFT.

Family and domain databases

CDDicd00212. PTS_IIB_glc. 1 hit.
Gene3Di3.30.1360.60. 1 hit.
InterProiIPR018113. PTrfase_EIIB_Cys.
IPR003352. PTS_EIIC.
IPR013013. PTS_EIIC_1.
IPR001996. PTS_IIB_1.
IPR011299. PTS_IIBC_glc.
[Graphical view]
PfamiPF00367. PTS_EIIB. 1 hit.
PF02378. PTS_EIIC. 1 hit.
[Graphical view]
SUPFAMiSSF55604. SSF55604. 1 hit.
TIGRFAMsiTIGR00826. EIIB_glc. 1 hit.
TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITEiPS51098. PTS_EIIB_TYPE_1. 1 hit.
PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit.
PS51103. PTS_EIIC_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTGCB_BUCAI
AccessioniPrimary (citable) accession number: P57437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.