ID   RLUC_BUCAI              Reviewed;         314 AA.
AC   P57430;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   27-MAR-2024, entry version 111.
DE   RecName: Full=Ribosomal large subunit pseudouridine synthase C;
DE            EC=5.4.99.24;
DE   AltName: Full=23S rRNA pseudouridine(955/2504/2580) synthase;
DE   AltName: Full=rRNA pseudouridylate synthase C;
DE   AltName: Full=rRNA-uridine isomerase C;
GN   Name=rluC; OrderedLocusNames=BU348;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Responsible for synthesis of pseudouridine from uracil at
CC       positions 955, 2504 and 2580 in 23S ribosomal RNA. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=uridine(955/2504/2580) in 23S rRNA =
CC         pseudouridine(955/2504/2580) in 23S rRNA; Xref=Rhea:RHEA:42528,
CC         Rhea:RHEA-COMP:10099, Rhea:RHEA-COMP:10100, ChEBI:CHEBI:65314,
CC         ChEBI:CHEBI:65315; EC=5.4.99.24;
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase RluA family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BA000003; BAB13053.1; -; Genomic_DNA.
DR   RefSeq; NP_240167.1; NC_002528.1.
DR   RefSeq; WP_009874303.1; NC_002528.1.
DR   AlphaFoldDB; P57430; -.
DR   SMR; P57430; -.
DR   STRING; 563178.BUAP5A_342; -.
DR   EnsemblBacteria; BAB13053; BAB13053; BAB13053.
DR   KEGG; buc:BU348; -.
DR   PATRIC; fig|107806.10.peg.360; -.
DR   eggNOG; COG0564; Bacteria.
DR   eggNOG; COG1188; Bacteria.
DR   HOGENOM; CLU_016902_1_1_6; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0120159; F:rRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0000455; P:enzyme-directed rRNA pseudouridine synthesis; IEA:UniProt.
DR   CDD; cd02869; PseudoU_synth_RluA_like; 1.
DR   CDD; cd00165; S4; 1.
DR   Gene3D; 3.30.2350.10; Pseudouridine synthase; 1.
DR   Gene3D; 3.10.290.10; RNA-binding S4 domain; 1.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR006224; PsdUridine_synth_RluA-like_CS.
DR   InterPro; IPR006225; PsdUridine_synth_RluC/D.
DR   InterPro; IPR006145; PsdUridine_synth_RsuA/RluA.
DR   InterPro; IPR002942; S4_RNA-bd.
DR   InterPro; IPR036986; S4_RNA-bd_sf.
DR   NCBIfam; TIGR00005; rluA_subfam; 1.
DR   PANTHER; PTHR21600; MITOCHONDRIAL RNA PSEUDOURIDINE SYNTHASE; 1.
DR   PANTHER; PTHR21600:SF93; PSEUDOURIDYLATE SYNTHASE RPUSD4, MITOCHONDRIAL; 1.
DR   Pfam; PF00849; PseudoU_synth_2; 1.
DR   Pfam; PF01479; S4; 1.
DR   SMART; SM00363; S4; 1.
DR   SUPFAM; SSF55174; Alpha-L RNA-binding motif; 1.
DR   SUPFAM; SSF55120; Pseudouridine synthase; 1.
DR   PROSITE; PS01129; PSI_RLU; 1.
DR   PROSITE; PS50889; S4; 1.
PE   3: Inferred from homology;
KW   Isomerase; Reference proteome; RNA-binding; rRNA processing.
FT   CHAIN           1..314
FT                   /note="Ribosomal large subunit pseudouridine synthase C"
FT                   /id="PRO_0000162664"
FT   DOMAIN          20..84
FT                   /note="S4 RNA-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00182"
FT   ACT_SITE        143
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   314 AA;  36614 MW;  4B0FC4D2FD085B08 CRC64;
     MTHKILPISI IYINKEMLNQ RIDNFMRSKF KSVPKSMIYR IIRTGKIRIN KKRIKPHYKL
     KIGDILKIPP IKILCDIKNT FFPLNHSTNL LNRILYEDSH LLIINKPSGI AVHGGSGLNF
     GVIEYFRKLR PLNKFLELVH RIDRDTSGVL ILAKKRTSLV SLHEQLREKK IKKEYIALVH
     GLWPDHIKKI SEPLLKIQFK NKQKMVLIDK KGKPSETYFQ IKKKFSSSTL LSIIPKTGRT
     HQIRVHALHA GHPIFFDKRY GKNDLDADIK NNHKINRLLL HSSGVHFIHP KNGNKIYIKA
     PLDIDFQNYL NTII
//