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P57429 (RNE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length902 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs By similarity. HAMAP-Rule MF_00970

Catalytic activity

Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions. HAMAP-Rule MF_00970

Cofactor

Binds 2 Zn2+ ions per homotetramer By similarity. HAMAP-Rule MF_00970

Binds 1 Mg2+ ion per subunit By similarity. HAMAP-Rule MF_00970

Subunit structure

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, Rnase E assembles into a homotetramer formed by a dimer of dimers By similarity.

Subcellular location

Cytoplasm By similarity. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00970.

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.

Contains 1 S1 motif domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 902902Ribonuclease E HAMAP-Rule MF_00970
PRO_0000097370

Regions

Domain39 – 11981S1 motif
Region404 – 4074Required for zinc-mediated homotetramerization and catalytic activity By similarity

Sites

Metal binding3031Magnesium; catalytic By similarity
Metal binding3461Magnesium; catalytic By similarity
Metal binding4041Zinc; shared with dimeric partner By similarity
Metal binding4071Zinc; shared with dimeric partner By similarity

Sequences

Sequence LengthMass (Da)Tools
P57429 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: C3FA00476E3C2E37

FASTA902104,007
        10         20         30         40         50         60 
MKRMLINATQ QEELRVALVD GQRLYDLDIE HSGSEQKKSN IYKGKITRIE PSLEAAFVDY 

        70         80         90        100        110        120 
GEEKNGFLPL KEISKNYFPE NHIETLGFNI KNVLQEGQEV IVQISKEERG TKGAALTTFI 

       130        140        150        160        170        180 
SLAGSYLVLM PNSPKSGGIS RRIEGNDRIA LKELLTLLEL PEEMSLIIRT AGAGKSIESL 

       190        200        210        220        230        240 
RWDLSLRLQH WKTIQIIAKS RTAPFLIHQE SNIIVRAFRD YLRQDIGEIL IDNPKILDLA 

       250        260        270        280        290        300 
RKHITFLGRP DFVNKIKLYS GEVPLFSYFQ IETQINSAFQ RKVRLPSGGS IMVDSTEALT 

       310        320        330        340        350        360 
AIDINSSRST SGTDIASTAF NTNLEAVDEI SRQLRLRDLG GLIVIDFIDM SAISHQRAIE 

       370        380        390        400        410        420 
NRLREIARDD RARIQIGQIS RFGLLEMSRQ RLSSSLGESS HHICPRCTGT GTIRDNESLS 

       430        440        450        460        470        480 
LSILRLIEEE ALKENTYEVR AIVPVEIACY LLNEKRDAVH AIEKRQAGGK TIIVPSKKMK 

       490        500        510        520        530        540 
TPHYSVSRIR KSESKNYTRY GLSNIRQSKI TSFLKKNLLK KKQKEILDVA NFNFYDNCYN 

       550        560        570        580        590        600 
KIQEAQENIL KKNNYNNILL KVLSNNRNFI FKMITWFKNS FFIKNMLITS DIFKKNTLKN 

       610        620        630        640        650        660 
TNNIFFKKKY SSLNKKNNNQ KKRVILSKLF EANIENIPLK NKKLDTSSAN YLYDNIERKK 

       670        680        690        700        710        720 
NITKKNDLIQ KNIHENSYLK HVLMNRYNVI NIINNNYIFY KIFNRTKIFK NQNTNNSFLK 

       730        740        750        760        770        780 
FSSVTSPIFI FSSVFSLELA LGKVWIKYPI AILDETKKQK KLNRKQILHI SSISETNTIV 

       790        800        810        820        830        840 
NKNNYSGIKK IKHETYSFKK YVKNNIQNQE VTQSQLIKRT KKRNVFILDK KNFLYKRTCN 

       850        860        870        880        890        900 
RKNKIHQSSA PITKISKQSD LNKKEKEFHY NLSMMKSSLR GKNSAGVHSA TNFSNSPVSK 


LK 

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References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13052.1.
RefSeqNP_240166.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57429.
SMRP57429. Positions 1-502.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP57429.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13052; BAB13052; BAB13052.
GeneID1110043.
KEGGbuc:BU347.
PATRIC21244228. VBIBucAph127364_0359.

Phylogenomic databases

eggNOGCOG1530.
HOGENOMHOG000258026.
KOK08300.
OMAEIACYLL.
OrthoDBEOG6PCPTH.
ProtClustDBCLSK873789.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-347-MONOMER.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00970. RNase_E.
InterProIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR022967. RNA-binding_domain_S1.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
[Graphical view]
PfamPF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTSM00316. S1. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00757. RNaseEG. 1 hit.
PROSITEPS50126. S1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNE_BUCAI
AccessionPrimary (citable) accession number: P57429
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names