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Protein

Ribonuclease E

Gene

rne

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per homotetramer.UniRule annotation
  • Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi303 – 3031Magnesium; catalyticUniRule annotation
Metal bindingi346 – 3461Magnesium; catalyticUniRule annotation
Metal bindingi404 – 4041Zinc; shared with dimeric partnerUniRule annotation
Metal bindingi407 – 4071Zinc; shared with dimeric partnerUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

rRNA processing, tRNA processing

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-347-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease EUniRule annotation (EC:3.1.26.12UniRule annotation)
Short name:
RNase EUniRule annotation
Gene namesi
Name:rneUniRule annotation
Ordered Locus Names:BU347
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation
  • Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 902902Ribonuclease EPRO_0000097370Add
BLAST

Proteomic databases

PRIDEiP57429.

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, Rnase E assembles into a homotetramer formed by a dimer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi107806.BU347.

Structurei

3D structure databases

ProteinModelPortaliP57429.
SMRiP57429. Positions 1-502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 11981S1 motifUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni404 – 4074Required for zinc-mediated homotetramerization and catalytic activityUniRule annotation

Sequence similaritiesi

Belongs to the RNase E/G family. RNase E subfamily.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1530.
HOGENOMiHOG000258026.
KOiK08300.
OMAiEIACYLL.
OrthoDBiEOG6PCPTH.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00970. RNase_E.
InterProiIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57429-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRMLINATQ QEELRVALVD GQRLYDLDIE HSGSEQKKSN IYKGKITRIE
60 70 80 90 100
PSLEAAFVDY GEEKNGFLPL KEISKNYFPE NHIETLGFNI KNVLQEGQEV
110 120 130 140 150
IVQISKEERG TKGAALTTFI SLAGSYLVLM PNSPKSGGIS RRIEGNDRIA
160 170 180 190 200
LKELLTLLEL PEEMSLIIRT AGAGKSIESL RWDLSLRLQH WKTIQIIAKS
210 220 230 240 250
RTAPFLIHQE SNIIVRAFRD YLRQDIGEIL IDNPKILDLA RKHITFLGRP
260 270 280 290 300
DFVNKIKLYS GEVPLFSYFQ IETQINSAFQ RKVRLPSGGS IMVDSTEALT
310 320 330 340 350
AIDINSSRST SGTDIASTAF NTNLEAVDEI SRQLRLRDLG GLIVIDFIDM
360 370 380 390 400
SAISHQRAIE NRLREIARDD RARIQIGQIS RFGLLEMSRQ RLSSSLGESS
410 420 430 440 450
HHICPRCTGT GTIRDNESLS LSILRLIEEE ALKENTYEVR AIVPVEIACY
460 470 480 490 500
LLNEKRDAVH AIEKRQAGGK TIIVPSKKMK TPHYSVSRIR KSESKNYTRY
510 520 530 540 550
GLSNIRQSKI TSFLKKNLLK KKQKEILDVA NFNFYDNCYN KIQEAQENIL
560 570 580 590 600
KKNNYNNILL KVLSNNRNFI FKMITWFKNS FFIKNMLITS DIFKKNTLKN
610 620 630 640 650
TNNIFFKKKY SSLNKKNNNQ KKRVILSKLF EANIENIPLK NKKLDTSSAN
660 670 680 690 700
YLYDNIERKK NITKKNDLIQ KNIHENSYLK HVLMNRYNVI NIINNNYIFY
710 720 730 740 750
KIFNRTKIFK NQNTNNSFLK FSSVTSPIFI FSSVFSLELA LGKVWIKYPI
760 770 780 790 800
AILDETKKQK KLNRKQILHI SSISETNTIV NKNNYSGIKK IKHETYSFKK
810 820 830 840 850
YVKNNIQNQE VTQSQLIKRT KKRNVFILDK KNFLYKRTCN RKNKIHQSSA
860 870 880 890 900
PITKISKQSD LNKKEKEFHY NLSMMKSSLR GKNSAGVHSA TNFSNSPVSK

LK
Length:902
Mass (Da):104,007
Last modified:December 1, 2000 - v1
Checksum:iC3FA00476E3C2E37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13052.1.
RefSeqiNP_240166.1. NC_002528.1.
WP_010896079.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13052; BAB13052; BAB13052.
GeneIDi1110043.
KEGGibuc:BU347.
PATRICi21244228. VBIBucAph127364_0359.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13052.1.
RefSeqiNP_240166.1. NC_002528.1.
WP_010896079.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57429.
SMRiP57429. Positions 1-502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU347.

Proteomic databases

PRIDEiP57429.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13052; BAB13052; BAB13052.
GeneIDi1110043.
KEGGibuc:BU347.
PATRICi21244228. VBIBucAph127364_0359.

Phylogenomic databases

eggNOGiCOG1530.
HOGENOMiHOG000258026.
KOiK08300.
OMAiEIACYLL.
OrthoDBiEOG6PCPTH.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-347-MONOMER.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00970. RNase_E.
InterProiIPR012340. NA-bd_OB-fold.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR019307. RNA-bd_AU-1/RNase_E/G.
IPR028878. RNase_E.
IPR004659. RNase_E/G.
IPR022967. S1_dom.
[Graphical view]
PfamiPF10150. RNase_E_G. 1 hit.
PF00575. S1. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
PROSITEiPS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiRNE_BUCAI
AccessioniPrimary (citable) accession number: P57429
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 24, 2015
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.