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P57429

- RNE_BUCAI

UniProt

P57429 - RNE_BUCAI

Protein

Ribonuclease E

Gene

rne

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.UniRule annotation

    Catalytic activityi

    Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.UniRule annotation

    Cofactori

    Binds 2 Zn2+ ions per homotetramer.UniRule annotation
    Binds 1 Mg2+ ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi303 – 3031Magnesium; catalyticUniRule annotation
    Metal bindingi346 – 3461Magnesium; catalyticUniRule annotation
    Metal bindingi404 – 4041Zinc; shared with dimeric partnerUniRule annotation
    Metal bindingi407 – 4071Zinc; shared with dimeric partnerUniRule annotation

    GO - Molecular functioni

    1. endoribonuclease activity Source: UniProtKB-HAMAP
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. ribonuclease E activity Source: InterPro
    4. RNA binding Source: UniProtKB-HAMAP
    5. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. rRNA processing Source: UniProtKB-HAMAP
    3. tRNA processing Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    rRNA processing, tRNA processing

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-347-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonuclease EUniRule annotation (EC:3.1.26.12UniRule annotation)
    Short name:
    RNase EUniRule annotation
    Gene namesi
    Name:rneUniRule annotation
    Ordered Locus Names:BU347
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation. Cell inner membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. cytoplasmic side of plasma membrane Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 902902Ribonuclease EPRO_0000097370Add
    BLAST

    Proteomic databases

    PRIDEiP57429.

    Interactioni

    Subunit structurei

    Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, Rnase E assembles into a homotetramer formed by a dimer of dimers.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57429.
    SMRiP57429. Positions 1-502.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 11981S1 motifUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni404 – 4074Required for zinc-mediated homotetramerization and catalytic activityUniRule annotation

    Sequence similaritiesi

    Belongs to the RNase E/G family. RNase E subfamily.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1530.
    HOGENOMiHOG000258026.
    KOiK08300.
    OMAiEIACYLL.
    OrthoDBiEOG6PCPTH.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    HAMAPiMF_00970. RNase_E.
    InterProiIPR012340. NA-bd_OB-fold.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR019307. RNA-bd_AU-1/RNase_E/G.
    IPR022967. RNA-binding_domain_S1.
    IPR028878. RNase_E.
    IPR004659. RNase_E/G.
    [Graphical view]
    PfamiPF10150. RNase_E_G. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view]
    SMARTiSM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    TIGRFAMsiTIGR00757. RNaseEG. 1 hit.
    PROSITEiPS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57429-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRMLINATQ QEELRVALVD GQRLYDLDIE HSGSEQKKSN IYKGKITRIE    50
    PSLEAAFVDY GEEKNGFLPL KEISKNYFPE NHIETLGFNI KNVLQEGQEV 100
    IVQISKEERG TKGAALTTFI SLAGSYLVLM PNSPKSGGIS RRIEGNDRIA 150
    LKELLTLLEL PEEMSLIIRT AGAGKSIESL RWDLSLRLQH WKTIQIIAKS 200
    RTAPFLIHQE SNIIVRAFRD YLRQDIGEIL IDNPKILDLA RKHITFLGRP 250
    DFVNKIKLYS GEVPLFSYFQ IETQINSAFQ RKVRLPSGGS IMVDSTEALT 300
    AIDINSSRST SGTDIASTAF NTNLEAVDEI SRQLRLRDLG GLIVIDFIDM 350
    SAISHQRAIE NRLREIARDD RARIQIGQIS RFGLLEMSRQ RLSSSLGESS 400
    HHICPRCTGT GTIRDNESLS LSILRLIEEE ALKENTYEVR AIVPVEIACY 450
    LLNEKRDAVH AIEKRQAGGK TIIVPSKKMK TPHYSVSRIR KSESKNYTRY 500
    GLSNIRQSKI TSFLKKNLLK KKQKEILDVA NFNFYDNCYN KIQEAQENIL 550
    KKNNYNNILL KVLSNNRNFI FKMITWFKNS FFIKNMLITS DIFKKNTLKN 600
    TNNIFFKKKY SSLNKKNNNQ KKRVILSKLF EANIENIPLK NKKLDTSSAN 650
    YLYDNIERKK NITKKNDLIQ KNIHENSYLK HVLMNRYNVI NIINNNYIFY 700
    KIFNRTKIFK NQNTNNSFLK FSSVTSPIFI FSSVFSLELA LGKVWIKYPI 750
    AILDETKKQK KLNRKQILHI SSISETNTIV NKNNYSGIKK IKHETYSFKK 800
    YVKNNIQNQE VTQSQLIKRT KKRNVFILDK KNFLYKRTCN RKNKIHQSSA 850
    PITKISKQSD LNKKEKEFHY NLSMMKSSLR GKNSAGVHSA TNFSNSPVSK 900
    LK 902
    Length:902
    Mass (Da):104,007
    Last modified:December 1, 2000 - v1
    Checksum:iC3FA00476E3C2E37
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13052.1.
    RefSeqiNP_240166.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13052; BAB13052; BAB13052.
    GeneIDi1110043.
    KEGGibuc:BU347.
    PATRICi21244228. VBIBucAph127364_0359.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13052.1 .
    RefSeqi NP_240166.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57429.
    SMRi P57429. Positions 1-502.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P57429.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13052 ; BAB13052 ; BAB13052 .
    GeneIDi 1110043.
    KEGGi buc:BU347.
    PATRICi 21244228. VBIBucAph127364_0359.

    Phylogenomic databases

    eggNOGi COG1530.
    HOGENOMi HOG000258026.
    KOi K08300.
    OMAi EIACYLL.
    OrthoDBi EOG6PCPTH.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-347-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    HAMAPi MF_00970. RNase_E.
    InterProi IPR012340. NA-bd_OB-fold.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR019307. RNA-bd_AU-1/RNase_E/G.
    IPR022967. RNA-binding_domain_S1.
    IPR028878. RNase_E.
    IPR004659. RNase_E/G.
    [Graphical view ]
    Pfami PF10150. RNase_E_G. 1 hit.
    PF00575. S1. 1 hit.
    [Graphical view ]
    SMARTi SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    TIGRFAMsi TIGR00757. RNaseEG. 1 hit.
    PROSITEi PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiRNE_BUCAI
    AccessioniPrimary (citable) accession number: P57429
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3