P57429 (RNE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease E
Short name=RNase E
EC=3.1.26.12

Gene names

Name:	rne
Ordered Locus Names:	BU347

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	902 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein matures 5S rRNA from its precursors from all the rRNA genes. It is the major endoribonuclease participating in mRNA turnover By similarity.
Catalytic activity	Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
Subunit structure	Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the RNase E/G family. RNase E subfamily. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 902	902	Ribonuclease E		PRO_0000097370
Regions
	Domain	39 – 119	81	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	P57429 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: C3FA00476E3C2E37	FASTA	902	104,007
	10 20 30 40 50 60 MKRMLINATQ QEELRVALVD GQRLYDLDIE HSGSEQKKSN IYKGKITRIE PSLEAAFVDY 70 80 90 100 110 120 GEEKNGFLPL KEISKNYFPE NHIETLGFNI KNVLQEGQEV IVQISKEERG TKGAALTTFI 130 140 150 160 170 180 SLAGSYLVLM PNSPKSGGIS RRIEGNDRIA LKELLTLLEL PEEMSLIIRT AGAGKSIESL 190 200 210 220 230 240 RWDLSLRLQH WKTIQIIAKS RTAPFLIHQE SNIIVRAFRD YLRQDIGEIL IDNPKILDLA 250 260 270 280 290 300 RKHITFLGRP DFVNKIKLYS GEVPLFSYFQ IETQINSAFQ RKVRLPSGGS IMVDSTEALT 310 320 330 340 350 360 AIDINSSRST SGTDIASTAF NTNLEAVDEI SRQLRLRDLG GLIVIDFIDM SAISHQRAIE 370 380 390 400 410 420 NRLREIARDD RARIQIGQIS RFGLLEMSRQ RLSSSLGESS HHICPRCTGT GTIRDNESLS 430 440 450 460 470 480 LSILRLIEEE ALKENTYEVR AIVPVEIACY LLNEKRDAVH AIEKRQAGGK TIIVPSKKMK 490 500 510 520 530 540 TPHYSVSRIR KSESKNYTRY GLSNIRQSKI TSFLKKNLLK KKQKEILDVA NFNFYDNCYN 550 560 570 580 590 600 KIQEAQENIL KKNNYNNILL KVLSNNRNFI FKMITWFKNS FFIKNMLITS DIFKKNTLKN 610 620 630 640 650 660 TNNIFFKKKY SSLNKKNNNQ KKRVILSKLF EANIENIPLK NKKLDTSSAN YLYDNIERKK 670 680 690 700 710 720 NITKKNDLIQ KNIHENSYLK HVLMNRYNVI NIINNNYIFY KIFNRTKIFK NQNTNNSFLK 730 740 750 760 770 780 FSSVTSPIFI FSSVFSLELA LGKVWIKYPI AILDETKKQK KLNRKQILHI SSISETNTIV 790 800 810 820 830 840 NKNNYSGIKK IKHETYSFKK YVKNNIQNQE VTQSQLIKRT KKRNVFILDK KNFLYKRTCN 850 860 870 880 890 900 RKNKIHQSSA PITKISKQSD LNKKEKEFHY NLSMMKSSLR GKNSAGVHSA TNFSNSPVSK LK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13052.1.
RefSeq	NP_240166.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57429.
SMR	P57429. Positions 1-502.
ModBase	Search...
Proteomic databases
PRIDE	P57429.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13052; BAB13052; BAB13052.
GeneID	1110043.
KEGG	buc:BU347.
PATRIC	21244228. VBIBucAph127364_0359.
Phylogenomic databases
eggNOG	COG1530.
HOGENOM	HOG000258026.
KO	K08300.
OMA	EIACYLL.
ProtClustDB	CLSK873789.
Family and domain databases
Gene3D	2.40.50.140. 1 hit.
InterPro	IPR012340. NA-bd_OB-fold. IPR003029. Rbsml_prot_S1_RNA-bd_dom. IPR019307. RNA-bd_AU-1/RNase_E/G. IPR022967. RNA-binding_domain_S1. IPR004659. RNase_E/G. [Graphical view]
Pfam	PF10150. RNase_E_G. 1 hit. PF00575. S1. 1 hit. [Graphical view]
SMART	SM00316. S1. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00757. RNaseEG. 1 hit.
PROSITE	PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNE_BUCAI

Accession

Primary (citable) accession number: P57429

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program