Reviewed, UniProtKB/Swiss-Prot P57416 (PYRC_BUCAI)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	BU334

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	Dihydroorotase		PRO_0000147203
Sites
	Metal binding	17	1	Zinc 1 By similarity
	Metal binding	19	1	Zinc 1 By similarity
	Metal binding	103	1	Zinc 1; via carbamate group By similarity
	Metal binding	103	1	Zinc 2; via carbamate group By similarity
	Metal binding	140	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 2 By similarity
	Metal binding	252	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	103	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57416-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 41D16017597B52DC	FASTA	350	40,416
	10 20 30 40 50 60 MSKFVKKIKI IKPDDWHVHL RDNEILNQVI KYTGKFYKRA VIMPNLNSPI TSCLKSIAYR 70 80 90 100 110 120 NRILKSMHLN YKFKPLMTCY LTNSTSPKEL EFGFSKKIFV AAKFYPNGCT TNSKTGIKKI 130 140 150 160 170 180 SDITPVLECM EKIGMPLLIH GEEINQNIDI YDREAKFIEK TLDPLRKKFP KLKIVLEHIT 190 200 210 220 230 240 TKESVEYIKN NDVNYLSATI TPHHLMLNRN DMFYGGIQPY LYCLPILKKN KHRMALRKAI 250 260 270 280 290 300 SNGDKHFFLG SDTAPHLHKN KINMLGCAGI FNAPSSLLSY VKVFEEMRAL KYLQSFCSEN 310 320 330 340 350 GPKFYNMPIN KETITIIKKP CKIIKKINVG RNVIIPFLSG EILNWSIESD

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13039.1.
RefSeq	NP_240153.1.
3D structure databases
HSSP	HSSP built from PDB template 1J79 based on UniProtKB P05020.
ModBase	Search...
Genome annotation databases
GeneID	1110030.
GenomeReviews	Gene locus BU334 in contig BA000003_GR.
KEGG	buc:BU334.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57416.
Enzyme and pathway databases
BioCyc	BSP107806:BU334-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_BUCAI

Accession

Primary (citable) accession number: P57416

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)