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P57404

- KPYK_BUCAI

UniProt

P57404 - KPYK_BUCAI

Protein

Pyruvate kinase

Gene

pykA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 84 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Enzyme regulationi

    Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361SubstrateBy similarity
    Metal bindingi38 – 381PotassiumBy similarity
    Metal bindingi40 – 401PotassiumBy similarity
    Metal bindingi70 – 701PotassiumBy similarity
    Sitei223 – 2231Transition state stabilizerBy similarity
    Metal bindingi225 – 2251MagnesiumBy similarity
    Binding sitei251 – 2511Substrate; via amide nitrogenBy similarity
    Metal bindingi252 – 2521MagnesiumBy similarity
    Binding sitei252 – 2521Substrate; via amide nitrogenBy similarity
    Binding sitei284 – 2841SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-319-MONOMER.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pykA
    Ordered Locus Names:BU319
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 480480Pyruvate kinasePRO_0000112058Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021558.
    KOiK00873.
    OMAiTPVYFDS.
    OrthoDBiEOG6GBMB0.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57404-1 [UniParc]FASTAAdd to Basket

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    MLNRLRRTKI VATLGPSTDI NNNLEKIIRS GVNVLRFNFS HGSKDEHKSR    50
    ANQAREIMTR LNFHIALLGD LQGPKIRISK FIKNNIFLNV GDFFILDANL 100
    DQNNGNQERV GIDYKQLPYD LKVGDVLLLD DGRIQLKVIK STGHEILTKV 150
    VIGGILSNNK GINKLGGGLS ADALTEKDKK DIILATEINV DYLAISFPRC 200
    SNDLKQARKL AKEFGSNAKI IAKIERAEAV LNQNTIEDII LASDAIMIAR 250
    GDLGVEIGDS ELAGIQKKLI RTARQLNRIV ITATQMMESM ITNPLPTRAE 300
    VMDVANAVLD GSDAVMLSAE TASGEYPAET VIKMAKICKG AEKVPSINVS 350
    RHRIHAKFDD IEEAIAMSAM YVANHLKGIT AIITMTESGK TALMTSRITS 400
    GLPIFALSKN KETLNLASLY RGVTPIYFNS KNNNFKAANE AIVLLRKRGF 450
    LCRGELVIIT QGDIMGKIGK TNTSRILKVV 480
    Length:480
    Mass (Da):52,691
    Last modified:December 1, 2000 - v1
    Checksum:i5C955A1A2C561969
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13027.1.
    RefSeqiNP_240141.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13027; BAB13027; BAB13027.
    GeneIDi1110018.
    KEGGibuc:BU319.
    PATRICi21244166. VBIBucAph127364_0331.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13027.1 .
    RefSeqi NP_240141.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57404.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13027 ; BAB13027 ; BAB13027 .
    GeneIDi 1110018.
    KEGGi buc:BU319.
    PATRICi 21244166. VBIBucAph127364_0331.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021558.
    KOi K00873.
    OMAi TPVYFDS.
    OrthoDBi EOG6GBMB0.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BioCyci BAPH107806:GBZJ-319-MONOMER.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiKPYK_BUCAI
    AccessioniPrimary (citable) accession number: P57404
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3