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P57404

- KPYK_BUCAI

UniProt

P57404 - KPYK_BUCAI

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Protein
Pyruvate kinase
Gene
pykA, BU319
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium By similarity.
Potassium By similarity.

Enzyme regulationi

Allosterically activated by AMP and by several sugar phosphates. Belongs to type II PK By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361Substrate By similarity
Metal bindingi38 – 381Potassium By similarity
Metal bindingi40 – 401Potassium By similarity
Metal bindingi70 – 701Potassium By similarity
Sitei223 – 2231Transition state stabilizer By similarity
Metal bindingi225 – 2251Magnesium By similarity
Binding sitei251 – 2511Substrate; via amide nitrogen By similarity
Metal bindingi252 – 2521Magnesium By similarity
Binding sitei252 – 2521Substrate; via amide nitrogen By similarity
Binding sitei284 – 2841Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-319-MONOMER.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pykA
Ordered Locus Names:BU319
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 480480Pyruvate kinase
PRO_0000112058Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57404.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021558.
KOiK00873.
OMAiTPVYFDS.
OrthoDBiEOG6GBMB0.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57404-1 [UniParc]FASTAAdd to Basket

« Hide

MLNRLRRTKI VATLGPSTDI NNNLEKIIRS GVNVLRFNFS HGSKDEHKSR    50
ANQAREIMTR LNFHIALLGD LQGPKIRISK FIKNNIFLNV GDFFILDANL 100
DQNNGNQERV GIDYKQLPYD LKVGDVLLLD DGRIQLKVIK STGHEILTKV 150
VIGGILSNNK GINKLGGGLS ADALTEKDKK DIILATEINV DYLAISFPRC 200
SNDLKQARKL AKEFGSNAKI IAKIERAEAV LNQNTIEDII LASDAIMIAR 250
GDLGVEIGDS ELAGIQKKLI RTARQLNRIV ITATQMMESM ITNPLPTRAE 300
VMDVANAVLD GSDAVMLSAE TASGEYPAET VIKMAKICKG AEKVPSINVS 350
RHRIHAKFDD IEEAIAMSAM YVANHLKGIT AIITMTESGK TALMTSRITS 400
GLPIFALSKN KETLNLASLY RGVTPIYFNS KNNNFKAANE AIVLLRKRGF 450
LCRGELVIIT QGDIMGKIGK TNTSRILKVV 480
Length:480
Mass (Da):52,691
Last modified:December 1, 2000 - v1
Checksum:i5C955A1A2C561969
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13027.1.
RefSeqiNP_240141.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13027; BAB13027; BAB13027.
GeneIDi1110018.
KEGGibuc:BU319.
PATRICi21244166. VBIBucAph127364_0331.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13027.1 .
RefSeqi NP_240141.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57404.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13027 ; BAB13027 ; BAB13027 .
GeneIDi 1110018.
KEGGi buc:BU319.
PATRICi 21244166. VBIBucAph127364_0331.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021558.
KOi K00873.
OMAi TPVYFDS.
OrthoDBi EOG6GBMB0.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BioCyci BAPH107806:GBZJ-319-MONOMER.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiKPYK_BUCAI
AccessioniPrimary (citable) accession number: P57404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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