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P57398

- SYS_BUCAI

UniProt

P57398 - SYS_BUCAI

Protein

Serine--tRNA ligase

Gene

serS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).UniRule annotation

    Catalytic activityi

    ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).UniRule annotation
    ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei287 – 2871SerineUniRule annotation
    Binding sitei387 – 3871SerineUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi264 – 2663ATPUniRule annotation
    Nucleotide bindingi351 – 3544ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. serine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. selenocysteine biosynthetic process Source: UniProtKB-HAMAP
    2. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
    3. seryl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-313-MONOMER.
    UniPathwayiUPA00906; UER00895.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine--tRNA ligaseUniRule annotation (EC:6.1.1.11UniRule annotation)
    Alternative name(s):
    Seryl-tRNA synthetaseUniRule annotation
    Short name:
    SerRSUniRule annotation
    Seryl-tRNA(Ser/Sec) synthetaseUniRule annotation
    Gene namesi
    Name:serSUniRule annotation
    Ordered Locus Names:BU313
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 427427Serine--tRNA ligasePRO_0000122018Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer. The tRNA molecule binds across the dimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57398.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni233 – 2353Serine bindingUniRule annotation

    Domaini

    Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0172.
    HOGENOMiHOG000035938.
    KOiK01875.
    OMAiAIAMSVP.
    OrthoDBiEOG61KBH9.

    Family and domain databases

    Gene3Di1.10.287.40. 1 hit.
    HAMAPiMF_00176. Ser_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR002317. Ser-tRNA-ligase_type_1.
    IPR015866. Ser-tRNA-synth_1_N.
    IPR010978. tRNA-bd_arm.
    [Graphical view]
    PANTHERiPTHR11778. PTHR11778. 1 hit.
    PfamiPF02403. Seryl_tRNA_N. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
    PRINTSiPR00981. TRNASYNTHSER.
    SUPFAMiSSF46589. SSF46589. 1 hit.
    TIGRFAMsiTIGR00414. serS. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57398-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLNPYLLRNE LHLTAKKLLK KGYKLNISKI SSMEEKRKTL QIQTENLQFK    50
    HNALSNLFKE NKNIKNKNEL LRHQVIQSSK DLNASKIELN SLKEKIHHFS 100
    MCIPNIPSDD VPEGNTSINN KEIKYWGQKK KYDFEIQDHI ELGKKFNELD 150
    WKSSAQMSGS RFVIMKGKIA LLHRALSQFM LDLHTLKHGY IESYVPYLVH 200
    SEALYGTGQL PKFSDDLFHI NLTDKKKYIL IPTGEVPLTN LVYDQIIDEK 250
    DLPIMLTAHT PCFRSEASSY GRDTKGLIRL HQFDKVELVQ IVKPEKSYEA 300
    LEKLTNHAEK VLQLLNLPYR KMLLCTGDTG FAAVKTYDLE VWFPSEKKYR 350
    EVSSCSNMSD FQARRIKARY RKKSEQKNFF VHTLNGSGLA IGRTLAAILE 400
    NYQHSNGRIE IPKVLQKKYM QGLEFIN 427
    Length:427
    Mass (Da):49,523
    Last modified:December 1, 2000 - v1
    Checksum:iA4B485CD1DBD4026
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13021.1.
    RefSeqiNP_240135.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13021; BAB13021; BAB13021.
    GeneIDi1110012.
    KEGGibuc:BU313.
    PATRICi21244154. VBIBucAph127364_0325.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13021.1 .
    RefSeqi NP_240135.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57398.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13021 ; BAB13021 ; BAB13021 .
    GeneIDi 1110012.
    KEGGi buc:BU313.
    PATRICi 21244154. VBIBucAph127364_0325.

    Phylogenomic databases

    eggNOGi COG0172.
    HOGENOMi HOG000035938.
    KOi K01875.
    OMAi AIAMSVP.
    OrthoDBi EOG61KBH9.

    Enzyme and pathway databases

    UniPathwayi UPA00906 ; UER00895 .
    BioCyci BAPH107806:GBZJ-313-MONOMER.

    Family and domain databases

    Gene3Di 1.10.287.40. 1 hit.
    HAMAPi MF_00176. Ser_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR002317. Ser-tRNA-ligase_type_1.
    IPR015866. Ser-tRNA-synth_1_N.
    IPR010978. tRNA-bd_arm.
    [Graphical view ]
    PANTHERi PTHR11778. PTHR11778. 1 hit.
    Pfami PF02403. Seryl_tRNA_N. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
    PRINTSi PR00981. TRNASYNTHSER.
    SUPFAMi SSF46589. SSF46589. 1 hit.
    TIGRFAMsi TIGR00414. serS. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiSYS_BUCAI
    AccessioniPrimary (citable) accession number: P57398
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3