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P57398

- SYS_BUCAI

UniProt

P57398 - SYS_BUCAI

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Protein

Serine--tRNA ligase

Gene

serS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).UniRule annotation

Catalytic activityi

ATP + L-serine + tRNA(Ser) = AMP + diphosphate + L-seryl-tRNA(Ser).UniRule annotation
ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec).UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei287 – 2871SerineUniRule annotation
Binding sitei387 – 3871SerineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi264 – 2663ATPUniRule annotation
Nucleotide bindingi351 – 3544ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. serine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. selenocysteine biosynthetic process Source: UniProtKB-HAMAP
  2. selenocysteinyl-tRNA(Sec) biosynthetic process Source: UniProtKB-UniPathway
  3. seryl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-313-MONOMER.
UniPathwayiUPA00906; UER00895.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine--tRNA ligaseUniRule annotation (EC:6.1.1.11UniRule annotation)
Alternative name(s):
Seryl-tRNA synthetaseUniRule annotation
Short name:
SerRSUniRule annotation
Seryl-tRNA(Ser/Sec) synthetaseUniRule annotation
Gene namesi
Name:serSUniRule annotation
Ordered Locus Names:BU313
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 427427Serine--tRNA ligasePRO_0000122018Add
BLAST

Interactioni

Subunit structurei

Homodimer. The tRNA molecule binds across the dimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57398.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni233 – 2353Serine bindingUniRule annotation

Domaini

Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0172.
HOGENOMiHOG000035938.
KOiK01875.
OMAiAIAMSVP.
OrthoDBiEOG61KBH9.

Family and domain databases

Gene3Di1.10.287.40. 1 hit.
HAMAPiMF_00176. Ser_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view]
PANTHERiPTHR11778. PTHR11778. 1 hit.
PfamiPF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view]
PIRSFiPIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSiPR00981. TRNASYNTHSER.
SUPFAMiSSF46589. SSF46589. 1 hit.
TIGRFAMsiTIGR00414. serS. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57398-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLNPYLLRNE LHLTAKKLLK KGYKLNISKI SSMEEKRKTL QIQTENLQFK
60 70 80 90 100
HNALSNLFKE NKNIKNKNEL LRHQVIQSSK DLNASKIELN SLKEKIHHFS
110 120 130 140 150
MCIPNIPSDD VPEGNTSINN KEIKYWGQKK KYDFEIQDHI ELGKKFNELD
160 170 180 190 200
WKSSAQMSGS RFVIMKGKIA LLHRALSQFM LDLHTLKHGY IESYVPYLVH
210 220 230 240 250
SEALYGTGQL PKFSDDLFHI NLTDKKKYIL IPTGEVPLTN LVYDQIIDEK
260 270 280 290 300
DLPIMLTAHT PCFRSEASSY GRDTKGLIRL HQFDKVELVQ IVKPEKSYEA
310 320 330 340 350
LEKLTNHAEK VLQLLNLPYR KMLLCTGDTG FAAVKTYDLE VWFPSEKKYR
360 370 380 390 400
EVSSCSNMSD FQARRIKARY RKKSEQKNFF VHTLNGSGLA IGRTLAAILE
410 420
NYQHSNGRIE IPKVLQKKYM QGLEFIN
Length:427
Mass (Da):49,523
Last modified:December 1, 2000 - v1
Checksum:iA4B485CD1DBD4026
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13021.1.
RefSeqiNP_240135.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13021; BAB13021; BAB13021.
GeneIDi1110012.
KEGGibuc:BU313.
PATRICi21244154. VBIBucAph127364_0325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB13021.1 .
RefSeqi NP_240135.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57398.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB13021 ; BAB13021 ; BAB13021 .
GeneIDi 1110012.
KEGGi buc:BU313.
PATRICi 21244154. VBIBucAph127364_0325.

Phylogenomic databases

eggNOGi COG0172.
HOGENOMi HOG000035938.
KOi K01875.
OMAi AIAMSVP.
OrthoDBi EOG61KBH9.

Enzyme and pathway databases

UniPathwayi UPA00906 ; UER00895 .
BioCyci BAPH107806:GBZJ-313-MONOMER.

Family and domain databases

Gene3Di 1.10.287.40. 1 hit.
HAMAPi MF_00176. Ser_tRNA_synth_type1.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR002317. Ser-tRNA-ligase_type_1.
IPR015866. Ser-tRNA-synth_1_N.
IPR010978. tRNA-bd_arm.
[Graphical view ]
PANTHERi PTHR11778. PTHR11778. 1 hit.
Pfami PF02403. Seryl_tRNA_N. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
[Graphical view ]
PIRSFi PIRSF001529. Ser-tRNA-synth_IIa. 1 hit.
PRINTSi PR00981. TRNASYNTHSER.
SUPFAMi SSF46589. SSF46589. 1 hit.
TIGRFAMsi TIGR00414. serS. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiSYS_BUCAI
AccessioniPrimary (citable) accession number: P57398
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3