Reviewed, UniProtKB/Swiss-Prot P57396 (AROA_BUCAI)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	BU311

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	427 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm Probable HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 427	427	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088235

Sequences

	Sequence		Length	Mass (Da)
	P57396-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 2FA4A2CFA8701285	FASTA	427	46,786
	10 20 30 40 50 60 MQNSLDLKPI SHVNGTVCLP GSKSISNRVL LLSSIAKGTT CLTNLLNSHD TQHMLNALKK 70 80 90 100 110 120 LGVRYNLSDD KKTCHVQGIG GPFHLSEAIS LYLGNAGTAI RPLLSVLSLH KNNILLNGDD 130 140 150 160 170 180 RMHERPIGDL VDALIQGGAV IEYKKNKGYP PICTKGGFLG GSIFLNGNIS SQFLTSLLIS 190 200 210 220 230 240 TPLALKDTTI FIKGNLVSKP YIDITLNLIK IFGVNIEHDS YNVFYIKGKQ QYKTPGKYTI 250 260 270 280 290 300 EGDASSASYF LAAAAIKGGS IKVTGVGKKS IQGDIEFANI LEKMGATIFW EDYSITCTRN 310 320 330 340 350 360 KLNAIDLDMN HIPDAAMTVA ILALFSKGTT IIRNIYNWRV KETDRLSAMT IELRKIGAIV 370 380 390 400 410 420 EEGRDFLSIS PPIFFQYSSI ETYNDHRMAM CFSLISLSGV GVNILNPNCI SKTFPSYFKD FLSISKI

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13019.1.
RefSeq	NP_240133.1.
3D structure databases
SMR	P57396. Positions 4-426.
ModBase	Search...
Genome annotation databases
GeneID	1110010.
GenomeReviews	Gene locus BU311 in contig BA000003_GR.
KEGG	buc:BU311.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
PhylomeDB	P57396.
Enzyme and pathway databases
BioCyc	BSP107806:BU311-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BUCAI

Accession

Primary (citable) accession number: P57396

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)