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P57392 (GLPF_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol uptake facilitator protein
Gene names
Name:glpF
Ordered Locus Names:BU306
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length263 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Domain

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similarities

Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
   Biological processGlycerol metabolism
Transport
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycerol metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 263263Glycerol uptake facilitator protein
PRO_0000064078

Regions

Topological domain1 – 77Cytoplasmic By similarity
Transmembrane8 – 3629Helical; Name=M1; By similarity
Topological domain37 – 415Extracellular By similarity
Transmembrane42 – 6221Helical; Name=M2; By similarity
Topological domain63 – 653Cytoplasmic By similarity
Intramembrane66 – 694 By similarity
Intramembrane70 – 8011Helical; Name=M3; By similarity
Topological domain81 – 866Cytoplasmic By similarity
Transmembrane87 – 11024Helical; Name=M4; By similarity
Topological domain111 – 14535Extracellular By similarity
Transmembrane146 – 17126Helical; Name=M5; By similarity
Topological domain172 – 18110Cytoplasmic By similarity
Transmembrane182 – 19817Helical; Name=M6; By similarity
Topological domain199 – 2024Extracellular By similarity
Intramembrane203 – 2064 By similarity
Intramembrane207 – 22014Helical; Name=M7; By similarity
Topological domain221 – 23616Extracellular By similarity
Transmembrane237 – 25923Helical; Name=M8; By similarity
Topological domain260 – 2634Cytoplasmic By similarity
Motif70 – 723NPA 1
Motif207 – 2093NPA 2

Sequences

Sequence LengthMass (Da)Tools
P57392 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 7F28F094BB56201B

FASTA26329,720
        10         20         30         40         50         60 
MNIYRKKNII KKCFMEFFGT GLVMFFGIGC LAASKLTNAN FTQFEISCIW GFGVSIAIYF 

        70         80         90        100        110        120 
SSSISGAHLN PAVTIFFWLS SKLNKRKVLP YIISQTLGSF FFTMLTYYLY NNLLISFERN 

       130        140        150        160        170        180 
NNVVRGTQES LNLASIFCVY PNYNNSFIYD FIIEIFSTAL FILIVLEFNN RNSNYFLYNR 

       190        200        210        220        230        240 
SVAPILTGFL VCMINLVINP LNNISLNPAR DLGPKILLSL TGWGLFSFTG GNDNILYCFI 

       250        260 
PIMGPILGAN LGGWIHKTLI NNS 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13015.1.
RefSeqNP_240129.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57392.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13015; BAB13015; BAB13015.
GeneID1110005.
KEGGbuc:BU306.
PATRIC21244138. VBIBucAph127364_0317.

Phylogenomic databases

eggNOGCOG0580.
HOGENOMHOG000288287.
KOK02440.
OMACFDGRKV.
OrthoDBEOG6TXQWK.
ProtClustDBCLSK315874.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-306-MONOMER.

Family and domain databases

Gene3D1.20.1080.10. 1 hit.
InterProIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERPTHR19139. PTHR19139. 1 hit.
PfamPF00230. MIP. 1 hit.
[Graphical view]
PRINTSPR00783. MINTRINSICP.
SUPFAMSSF81338. SSF81338. 1 hit.
PROSITEPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPF_BUCAI
AccessionPrimary (citable) accession number: P57392
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names