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Protein

Glycerol uptake facilitator protein

Gene

glpF

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane (By similarity).By similarity

GO - Molecular functioni

  1. transporter activity Source: InterPro

GO - Biological processi

  1. glycerol metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Glycerol metabolism, Transport

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-306-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol uptake facilitator protein
Gene namesi
Name:glpF
Ordered Locus Names:BU306
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicBy similarity
Transmembranei8 – 3629Helical; Name=M1By similarityAdd
BLAST
Topological domaini37 – 415ExtracellularBy similarity
Transmembranei42 – 6221Helical; Name=M2By similarityAdd
BLAST
Topological domaini63 – 653CytoplasmicBy similarity
Intramembranei66 – 694By similarity
Intramembranei70 – 8011Helical; Name=M3By similarityAdd
BLAST
Topological domaini81 – 866CytoplasmicBy similarity
Transmembranei87 – 11024Helical; Name=M4By similarityAdd
BLAST
Topological domaini111 – 14535ExtracellularBy similarityAdd
BLAST
Transmembranei146 – 17126Helical; Name=M5By similarityAdd
BLAST
Topological domaini172 – 18110CytoplasmicBy similarity
Transmembranei182 – 19817Helical; Name=M6By similarityAdd
BLAST
Topological domaini199 – 2024ExtracellularBy similarity
Intramembranei203 – 2064By similarity
Intramembranei207 – 22014Helical; Name=M7By similarityAdd
BLAST
Topological domaini221 – 23616ExtracellularBy similarityAdd
BLAST
Transmembranei237 – 25923Helical; Name=M8By similarityAdd
BLAST
Topological domaini260 – 2634CytoplasmicBy similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263Glycerol uptake facilitator proteinPRO_0000064078Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57392.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 723NPA 1
Motifi207 – 2093NPA 2

Domaini

Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0580.
HOGENOMiHOG000288287.
KOiK02440.
OMAiIGRNLPC.
OrthoDBiEOG6TXQWK.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57392-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNIYRKKNII KKCFMEFFGT GLVMFFGIGC LAASKLTNAN FTQFEISCIW
60 70 80 90 100
GFGVSIAIYF SSSISGAHLN PAVTIFFWLS SKLNKRKVLP YIISQTLGSF
110 120 130 140 150
FFTMLTYYLY NNLLISFERN NNVVRGTQES LNLASIFCVY PNYNNSFIYD
160 170 180 190 200
FIIEIFSTAL FILIVLEFNN RNSNYFLYNR SVAPILTGFL VCMINLVINP
210 220 230 240 250
LNNISLNPAR DLGPKILLSL TGWGLFSFTG GNDNILYCFI PIMGPILGAN
260
LGGWIHKTLI NNS
Length:263
Mass (Da):29,720
Last modified:December 1, 2000 - v1
Checksum:i7F28F094BB56201B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13015.1.
RefSeqiNP_240129.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB13015; BAB13015; BAB13015.
GeneIDi1110005.
KEGGibuc:BU306.
PATRICi21244138. VBIBucAph127364_0317.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB13015.1.
RefSeqiNP_240129.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57392.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB13015; BAB13015; BAB13015.
GeneIDi1110005.
KEGGibuc:BU306.
PATRICi21244138. VBIBucAph127364_0317.

Phylogenomic databases

eggNOGiCOG0580.
HOGENOMiHOG000288287.
KOiK02440.
OMAiIGRNLPC.
OrthoDBiEOG6TXQWK.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-306-MONOMER.

Family and domain databases

Gene3Di1.20.1080.10. 1 hit.
InterProiIPR023271. Aquaporin-like.
IPR000425. MIP.
IPR022357. MIP_CS.
[Graphical view]
PANTHERiPTHR19139. PTHR19139. 1 hit.
PfamiPF00230. MIP. 1 hit.
[Graphical view]
PRINTSiPR00783. MINTRINSICP.
SUPFAMiSSF81338. SSF81338. 1 hit.
PROSITEiPS00221. MIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiGLPF_BUCAI
AccessioniPrimary (citable) accession number: P57392
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: January 7, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.