P57392 (GLPF_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol uptake facilitator protein

Gene names

Name:	glpF
Ordered Locus Names:	BU306

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Glycerol metabolism Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 263	263	Glycerol uptake facilitator protein		PRO_0000064078
Regions
	Topological domain	1 – 7	7	Cytoplasmic By similarity
	Transmembrane	8 – 36	29	Helical; Name=M1; By similarity
	Topological domain	37 – 41	5	Extracellular By similarity
	Transmembrane	42 – 62	21	Helical; Name=M2; By similarity
	Topological domain	63 – 65	3	Cytoplasmic By similarity
	Intramembrane	66 – 69	4	By similarity
	Intramembrane	70 – 80	11	Helical; Name=M3; By similarity
	Topological domain	81 – 86	6	Cytoplasmic By similarity
	Transmembrane	87 – 110	24	Helical; Name=M4; By similarity
	Topological domain	111 – 145	35	Extracellular By similarity
	Transmembrane	146 – 171	26	Helical; Name=M5; By similarity
	Topological domain	172 – 181	10	Cytoplasmic By similarity
	Transmembrane	182 – 198	17	Helical; Name=M6; By similarity
	Topological domain	199 – 202	4	Extracellular By similarity
	Intramembrane	203 – 206	4	By similarity
	Intramembrane	207 – 220	14	Helical; Name=M7; By similarity
	Topological domain	221 – 236	16	Extracellular By similarity
	Transmembrane	237 – 259	23	Helical; Name=M8; By similarity
	Topological domain	260 – 263	4	Cytoplasmic By similarity
	Motif	70 – 72	3	NPA 1
	Motif	207 – 209	3	NPA 2

Sequences

	Sequence		Length	Mass (Da)
	P57392 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 7F28F094BB56201B	FASTA	263	29,720
	10 20 30 40 50 60 MNIYRKKNII KKCFMEFFGT GLVMFFGIGC LAASKLTNAN FTQFEISCIW GFGVSIAIYF 70 80 90 100 110 120 SSSISGAHLN PAVTIFFWLS SKLNKRKVLP YIISQTLGSF FFTMLTYYLY NNLLISFERN 130 140 150 160 170 180 NNVVRGTQES LNLASIFCVY PNYNNSFIYD FIIEIFSTAL FILIVLEFNN RNSNYFLYNR 190 200 210 220 230 240 SVAPILTGFL VCMINLVINP LNNISLNPAR DLGPKILLSL TGWGLFSFTG GNDNILYCFI 250 260 PIMGPILGAN LGGWIHKTLI NNS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13015.1.
RefSeq	NP_240129.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57392.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13015; BAB13015; BAB13015.
GeneID	1110005.
KEGG	buc:BU306.
PATRIC	21244138. VBIBucAph127364_0317.
Phylogenomic databases
eggNOG	COG0580.
HOGENOM	HOG000288287.
KO	K02440.
OMA	CTYPAEF.
ProtClustDB	CLSK315874.
Family and domain databases
Gene3D	1.20.1080.10. 1 hit.
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
PANTHER	PTHR19139. PTHR19139. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPF_BUCAI

Accession

Primary (citable) accession number: P57392

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program