Reviewed, UniProtKB/Swiss-Prot P57388 (ODO1_BUCAI)

Names and origin

Protein names

Recommended name:
    2-oxoglutarate dehydrogenase E1 component
    EC=1.2.4.2
Alternative name(s):
    Alpha-ketoglutarate dehydrogenase

Gene names

Name:	sucA
Ordered Locus Names:	BU302

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	909 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the alpha-ketoglutarate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxoglutarate dehydrogenase (succinyl-transferring) activity Inferred from electronic annotation. Source: InterPro thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 909	909	2-oxoglutarate dehydrogenase E1 component		PRO_0000162187

Sequences

	Sequence		Length	Mass (Da)
	P57388-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: AE739F8CD9F9CF8B	FASTA	909	105,807
	10 20 30 40 50 60 MDKNKIEYWL NSSWLSRENQ NYIETIYKSF LTNAQSIDDM WHKAFLEFSE EQKNTYERNN 70 80 90 100 110 120 TKNNKYLLIK KIDHMIHAFR SEGYQQSLID PLKLKKRTKI HDLDLSFYNF TEEETRQTVE 130 140 150 160 170 180 INFKNCTNFR TNIISLYKIL YKKYCGSIGF EYMYVNNLLE KQWITNHIES FFNENVFTIE 190 200 210 220 230 240 EKINFLKELT YAETLEKYIG KKFPGAKRFS LEGAETLIPV LHEVIRFSKK NNISKIVLGM 250 260 270 280 290 300 AHRGRLNVLI NVLNKSPKVL FDEFSNLNLF QKISGDVKYH MGGTAEIQYE KKIIFHMACN 310 320 330 340 350 360 PSHLEIINPV VSGISRSYID NMKNIDNEVL PISIHGDASV IGQGVVQETL NMSQTEGYKV 370 380 390 400 410 420 GGTVHIIINN QIGFTTSNPK HLRSSEYCTD VAKIIQAPVF HVNADDLEAS IFAIQLALHF 430 440 450 460 470 480 RKIFKKDVFI DLVCYRRNGH NEVDEPSVTQ PIMYQKIKNH PTSRTIYSDV LISKKIITSE 490 500 510 520 530 540 KNQEIMNQYL SKLQKGHYIF SKSKNIHFKN EFFLEEKKIK KIKKDVNFSD LKNLACLINQ 550 560 570 580 590 600 IPDSVKMHQR VKKIYEERLE MAQRLKLFDW GAAETLAYAT ILNEGISCRI SGEDVSRGTF 610 620 630 640 650 660 FHRHAFIHNQ INGSIYIPLN NISKKQGKFQ IWDSVLSEEA VLAFEYGYSL SSPNTLTIWE 670 680 690 700 710 720 AQFGDFINGA QIVIDQFISS GEQKWNKKSN LVVLLPHGYE GQGPEHSSSR IERFLQLCAE 730 740 750 760 770 780 ENMQICIPTT SSQIFHIFRK QIFDKILKPL IIFTPKSLLR NPMASSSFDD LVYGKFQKIL 790 800 810 820 830 840 DEVDNVNKKE IRLIFCSGKI YYDLLRNRRE KKINSIILIR IEQLYPFPEG EILKILKNYF 850 860 870 880 890 900 YIKDFIWCQE EPYNQGAWFY IKDCLSNILP LDASLKYIGR SSSASPAVGY ISIHKKQQEK IIYNALNIN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13011.1.
RefSeq	NP_240125.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1110001.
GenomeReviews	Gene locus BU302 in contig BA000003_GR.
KEGG	buc:BU302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57388.
Enzyme and pathway databases
BioCyc	BSP107806:BU302-MON.
Family and domain databases
InterPro	IPR011603. 2oxoglutarate_DHase_E1. IPR001017. DHase_E1. IPR005475. Transketo_Cen_R. [Graphical view]
PANTHER	PTHR23152. 2oxoglutarate_DH_E1. 1 hit.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. [Graphical view]
PIRSF	PIRSF000157. Oxoglu_dh_E1. 1 hit.
TIGRFAMs	TIGR00239. 2oxo_dh_E1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODO1_BUCAI

Accession

Primary (citable) accession number: P57388

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 25, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)