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P57384

- G3P_BUCAI

UniProt

P57384 - G3P_BUCAI

Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gapA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADBy similarity
    Binding sitei78 – 781NAD; via carbonyl oxygenBy similarity
    Active sitei150 – 1501NucleophilePROSITE-ProRule annotation
    Sitei177 – 1771Activates thiol group during catalysisBy similarity
    Binding sitei180 – 1801Glyceraldehyde 3-phosphateBy similarity
    Binding sitei232 – 2321Glyceraldehyde 3-phosphateBy similarity
    Binding sitei314 – 3141NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi12 – 132NADBy similarity

    GO - Molecular functioni

    1. glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: UniProtKB-EC
    2. NAD binding Source: InterPro
    3. NADP binding Source: InterPro

    GO - Biological processi

    1. glucose metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-298-MONOMER.
    UniPathwayiUPA00109; UER00184.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase (EC:1.2.1.12)
    Short name:
    GAPDH
    Gene namesi
    Name:gapA
    Ordered Locus Names:BU298
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 332332Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145638Add
    BLAST

    Proteomic databases

    PRIDEiP57384.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57384.
    SMRiP57384. Positions 2-331.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni149 – 1513Glyceraldehyde 3-phosphate bindingBy similarity
    Regioni209 – 2102Glyceraldehyde 3-phosphate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0057.
    HOGENOMiHOG000071678.
    KOiK00134.
    OMAiNDKWGIE.
    OrthoDBiEOG66TG3S.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10836. PTHR10836. 1 hit.
    PfamiPF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000149. GAP_DH. 1 hit.
    PRINTSiPR00078. G3PDHDRGNASE.
    SMARTiSM00846. Gp_dh_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
    PROSITEiPS00071. GAPDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57384-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTIKVGINGF GRIGRVLFRL AQKRSDIEII AINDLLAPEY IAYMLKYDST    50
    HGVFKKHITF DKDNIIINGK NIRVTSIKDP EKLMWHDLSI DVVIESTGLF 100
    LKREQAYKHI LAGSKKVVVT GPSKDDIPMF VRGANFHKYQ GESIVSNASC 150
    TTNCLAPLSK VIDDNFGIIE GLMTTVHAST ATQKIVDGAS DKDWRGGRGA 200
    LQNIIPSSTG AAIAVGKVLP NLNGKLTGMA FRVPVSNVSV VDLTVRYKKS 250
    ATYSEICQKI QQASQKEMKG IMGYTEDEVV STDFNGEELT SIFDAKAGLS 300
    LNKNFVKLIA WYDNETGYSS KVLDLVSLVS KK 332
    Length:332
    Mass (Da):36,475
    Last modified:December 1, 2000 - v1
    Checksum:iBDA01457D9EB81E4
    GO

    Sequence cautioni

    The sequence BAB13007.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13007.1. Different initiation.
    RefSeqiNP_240121.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB13007; BAB13007; BAB13007.
    GeneIDi1109997.
    KEGGibuc:BU298.
    PATRICi21244122. VBIBucAph127364_0309.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB13007.1 . Different initiation.
    RefSeqi NP_240121.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57384.
    SMRi P57384. Positions 2-331.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P57384.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB13007 ; BAB13007 ; BAB13007 .
    GeneIDi 1109997.
    KEGGi buc:BU298.
    PATRICi 21244122. VBIBucAph127364_0309.

    Phylogenomic databases

    eggNOGi COG0057.
    HOGENOMi HOG000071678.
    KOi K00134.
    OMAi NDKWGIE.
    OrthoDBi EOG66TG3S.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00184 .
    BioCyci BAPH107806:GBZJ-298-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR020831. GlycerAld/Erythrose_P_DH.
    IPR020830. GlycerAld_3-P_DH_AS.
    IPR020829. GlycerAld_3-P_DH_cat.
    IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
    IPR006424. Glyceraldehyde-3-P_DH_1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10836. PTHR10836. 1 hit.
    Pfami PF02800. Gp_dh_C. 1 hit.
    PF00044. Gp_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000149. GAP_DH. 1 hit.
    PRINTSi PR00078. G3PDHDRGNASE.
    SMARTi SM00846. Gp_dh_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01534. GAPDH-I. 1 hit.
    PROSITEi PS00071. GAPDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiG3P_BUCAI
    AccessioniPrimary (citable) accession number: P57384
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3