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P57378 (BIOB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 343343Biotin synthase HAMAP-Rule MF_01694
PRO_0000185547

Sites

Metal binding511Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding551Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding581Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding951Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1261Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1861Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2581Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P57378 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: F86E87C58479B990

FASTA34339,213
        10         20         30         40         50         60 
MKKKWTLEET KMLFKKPFFN LMFQAQEEHR KNFDPNTIQI STLLSIKTGS CPEDCKYCPQ 

        70         80         90        100        110        120 
SSRYKTGLKK EPLLEIEQIL SAAKKAKDSG SSRFCMGAAW KNPKEKDMPY LEEIIKKIKE 

       130        140        150        160        170        180 
MGMETCMTLG TLNSTQAKKL ADAGLDFYNH NLDTSKNFYN NIITTRTYQE RLHTLHAVRS 

       190        200        210        220        230        240 
SGMKVCSGGI IGLGEKKQDR IELLMELSNL SIQPESVPIN MLVKIPGTPM ADNKDVEPFD 

       250        260        270        280        290        300 
FIRIIAVARI MMPKSYIRLS AGRHKMNDQT QAMCFMAGAN SIFYGCKLLT SDNPEEKHDL 

       310        320        330        340 
ELFKKLDLIP ENKTQTLLKE DEYKTIIKSS KIKKDQYYNA AII 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB13001.1.
RefSeqNP_240115.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57378.
SMRP57378. Positions 2-311.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP57378.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB13001; BAB13001; BAB13001.
GeneID1109990.
KEGGbuc:BU291.
PATRIC21244106. VBIBucAph127364_0301.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMAADRFCMG.
OrthoDBEOG622PMP.
ProtClustDBCLSK2300099.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-291-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_BUCAI
AccessionPrimary (citable) accession number: P57378
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names