P57378 (BIOB_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Biotin synthase
EC=2.8.1.6

Gene names

Name:	bioB
Ordered Locus Names:	BU291

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694
Catalytic activity	Dethiobiotin + sulfur + 2 S-adenosyl-L-methionine = biotin + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694
Subunit structure	Homodimer By similarity. HAMAP-Rule MF_01694
Sequence similarities	Belongs to the radical SAM superfamily. Biotin synthase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW biotin synthase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Biotin synthase HAMAP-Rule MF_01694		PRO_0000185547
Sites
	Metal binding	51	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	55	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	58	1	Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	95	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	126	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	186	1	Iron-sulfur 2 (2Fe-2S) By similarity
	Metal binding	258	1	Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57378 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: F86E87C58479B990	FASTA	343	39,213
	10 20 30 40 50 60 MKKKWTLEET KMLFKKPFFN LMFQAQEEHR KNFDPNTIQI STLLSIKTGS CPEDCKYCPQ 70 80 90 100 110 120 SSRYKTGLKK EPLLEIEQIL SAAKKAKDSG SSRFCMGAAW KNPKEKDMPY LEEIIKKIKE 130 140 150 160 170 180 MGMETCMTLG TLNSTQAKKL ADAGLDFYNH NLDTSKNFYN NIITTRTYQE RLHTLHAVRS 190 200 210 220 230 240 SGMKVCSGGI IGLGEKKQDR IELLMELSNL SIQPESVPIN MLVKIPGTPM ADNKDVEPFD 250 260 270 280 290 300 FIRIIAVARI MMPKSYIRLS AGRHKMNDQT QAMCFMAGAN SIFYGCKLLT SDNPEEKHDL 310 320 330 340 ELFKKLDLIP ENKTQTLLKE DEYKTIIKSS KIKKDQYYNA AII

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB13001.1.
RefSeq	NP_240115.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57378.
SMR	P57378. Positions 2-311.
ModBase	Search...
Proteomic databases
PRIDE	P57378.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB13001; BAB13001; BAB13001.
GeneID	1109990.
KEGG	buc:BU291.
PATRIC	21244106. VBIBucAph127364_0301.
Phylogenomic databases
eggNOG	COG0502.
HOGENOM	HOG000239957.
KO	K01012.
OMA	RIMMPAS.
ProtClustDB	CLSK2300099.
Enzyme and pathway databases
BioCyc	BAPH107806:GBZJ-291-MONOMER.
UniPathway	UPA00078; UER00162.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01694. BioB.
InterPro	IPR013785. Aldolase_TIM. IPR010722. Biotin/thiamin_synth-assoc. IPR002684. Biotin_synth/BioAB. IPR024177. Biotin_synthase. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF06968. BATS. 1 hit. PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF001619. Biotin_synth. 1 hit.
SMART	SM00876. BATS. 1 hit. SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00433. bioB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOB_BUCAI

Accession

Primary (citable) accession number: P57378

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 29, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program