Reviewed, UniProtKB/Swiss-Prot P57377 (BIOD_BUCAI)

Names and origin

Protein names

Recommended name:
    Dethiobiotin synthetase
    EC=6.3.3.3
Alternative name(s):
    Dethiobiotin synthase
    DTB synthetase
      Short name=DTBS

Gene names

Name:	bioD
Ordered Locus Names:	BU290

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Dethiobiotin synthetase HAMAP MF_00336		PRO_0000187952
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57377-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: CA5125598895921C	FASTA	224	25,165
	10 20 30 40 50 60 MIKKFFITGT DTNVGKTIVS SILLKKATMS GYQTAGYKPV SSGGQKKSSG FFNQDAILLK 70 80 90 100 110 120 KSSSIILSDR EVNPIAFFEN APPHILSKFQ KRSIKKEELS LGLNNITKKS NWILVEGAGG 130 140 150 160 170 180 WYTPLSCKDT FSSWVKQEKL TVIIIIAIKL GCINHAILTE KAIISDQIKC GGWIANNIFP 190 200 210 220 KDKYNMHYIQ TLLNYIKSPF LGVVPYFKNK NRINFKKIKI KLPK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB13000.1.
RefSeq	NP_240114.1.
3D structure databases
HSSP	HSSP built from PDB template 1DAI based on UniProtKB P13000.
ModBase	Search...
Genome annotation databases
GeneID	1109989.
GenomeReviews	Gene locus BU290 in contig BA000003_GR.
KEGG	buc:BU290.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57377.
OMA	CLIEGAG.
Enzyme and pathway databases
BioCyc	BSP107806:BU290-MON.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BUCAI

Accession

Primary (citable) accession number: P57377

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)