Reviewed, UniProtKB/Swiss-Prot P57371 (TOP1_BUCAI)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	BU284

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	861 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Repeat Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 861	861	DNA topoisomerase 1		PRO_0000145144
Regions
	Zinc finger	596 – 628	33	C4-type 1
	Zinc finger	658 – 685	28	C4-type 2
	Zinc finger	707 – 732	26	C4-type 3
Sites
	Active site	318	1	For DNA cleavage activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57371-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 903BBF2EA5265599	FASTA	861	99,740
	10 20 30 40 50 60 MQKSLVIVES PAKAKTINQY LGSEYIVKSS IGHIRDLTKG KLYNKEKNKK FLNENFIEKT 70 80 90 100 110 120 NENKILIKQM GIDPYQNWKF EYHILPGKEK IISELKYIAN QVKHIYLATD LDREGEAIAW 130 140 150 160 170 180 HLKEVIGGDS SKFSRVVFNE ITQHSIQKAF KNVGHINMNR VHAQQARRFM DRIVGYMISP 190 200 210 220 230 240 LLWKKIARGL SAGRVQSVAV RIIADRESII KNFVPEEYWK LDVSLISQDK KKINMDVTHY 250 260 270 280 290 300 NNKKFRPINE NEVSFAVEKI QKSSCIVKNY EEKISYLKAP APFITSTLQQ SASLRLGFSV 310 320 330 340 350 360 KKTMFLAQKL YEEGYITYMR TDSNYLSEYA IKKVRKYIKS NYGSNYLPKE PNIYSNEKHS 370 380 390 400 410 420 QEAHEAIRPS DIKIKNIDSD HLNSSAKKLY ELIWNQFLAS QMKSVKYKSI TVTVLADMFK 430 440 450 460 470 480 LQKSERIVMF QGWNKVLIEE KNVFSQFPIL QTGSQLFINK VTPSQKFTKP PPRFSEASLV 490 500 510 520 530 540 RELEKKGIGR PSTYSAITSK IQDRGYVKIK KNKFYAEKMG EILTIRLKKS FSNLIDYNFT 550 560 570 580 590 600 AHMEKKLDQV AENKVTWRYL LDDFFKKFSE QLEQAKKSPE EGGMELNNIV PTSLNCPICC 610 620 630 640 650 660 KKMGIKTAIT GVFLSCLGYN NTDNKKRCKK TINLITLNDF NKEQDNQKKI SLQLIQKCDI 670 680 690 700 710 720 CNMYMDSYFI NEKLKLHICA NNPSCSGYKF EKGVFKSPIY LSKTIQCEKC YNNMKLKIGP 730 740 750 760 770 780 FGKFFTCINK ICKNTRKILP NGEISDPKLE PIPFPELLCK QSDAWFVLRE GISGIFFAAN 790 800 810 820 830 840 TFPKSRETRS PFVEELVRFQ HLLPEKIYYL SSAPVIDNYG NKTIVCFDKK KKTHYIASKK 850 860 DGKFTGWSAV FIDQKWCVIN K

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12994.1.
RefSeq	NP_240108.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	1109983.
GenomeReviews	Gene locus BU284 in contig BA000003_GR.
KEGG	buc:BU284.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57371.
OMA	INENEVS.
Enzyme and pathway databases
BioCyc	BSP107806:BU284-MON.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR013263. TopoI_Znr_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF08272. Topo_Zn_Ribbon. 2 hits. PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_BUCAI

Accession

Primary (citable) accession number: P57371

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)