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P57371

- TOP1_BUCAI

UniProt

P57371 - TOP1_BUCAI

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Protein
DNA topoisomerase 1
Gene
topA, BU284
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi9 – 91Magnesium 1; catalytic By similarity
Sitei33 – 331Interaction with DNA By similarity
Metal bindingi110 – 1101Magnesium 1; catalytic By similarity
Metal bindingi110 – 1101Magnesium 2 By similarity
Metal bindingi112 – 1121Magnesium 2 By similarity
Sitei167 – 1671Interaction with DNA By similarity
Sitei168 – 1681Interaction with DNA By similarity
Sitei171 – 1711Interaction with DNA By similarity
Sitei176 – 1761Interaction with DNA By similarity
Sitei183 – 1831Interaction with DNA By similarity
Active sitei318 – 3181O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei320 – 3201Interaction with DNA By similarity
Sitei504 – 5041Interaction with DNA By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri596 – 62833C4-type 1UniRule annotation
Add
BLAST
Zinc fingeri658 – 68528C4-type 2UniRule annotation
Add
BLAST
Zinc fingeri707 – 73226C4-type 3UniRule annotation
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
  5. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-284-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:BU284
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. chromosome Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 861861DNA topoisomerase 1UniRule annotation
PRO_0000145144Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57371.
SMRiP57371. Positions 2-569, 741-856.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 141139Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 1966Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000004018.
KOiK03168.
OMAiVVECDKC.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57371-1 [UniParc]FASTAAdd to Basket

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MQKSLVIVES PAKAKTINQY LGSEYIVKSS IGHIRDLTKG KLYNKEKNKK    50
FLNENFIEKT NENKILIKQM GIDPYQNWKF EYHILPGKEK IISELKYIAN 100
QVKHIYLATD LDREGEAIAW HLKEVIGGDS SKFSRVVFNE ITQHSIQKAF 150
KNVGHINMNR VHAQQARRFM DRIVGYMISP LLWKKIARGL SAGRVQSVAV 200
RIIADRESII KNFVPEEYWK LDVSLISQDK KKINMDVTHY NNKKFRPINE 250
NEVSFAVEKI QKSSCIVKNY EEKISYLKAP APFITSTLQQ SASLRLGFSV 300
KKTMFLAQKL YEEGYITYMR TDSNYLSEYA IKKVRKYIKS NYGSNYLPKE 350
PNIYSNEKHS QEAHEAIRPS DIKIKNIDSD HLNSSAKKLY ELIWNQFLAS 400
QMKSVKYKSI TVTVLADMFK LQKSERIVMF QGWNKVLIEE KNVFSQFPIL 450
QTGSQLFINK VTPSQKFTKP PPRFSEASLV RELEKKGIGR PSTYSAITSK 500
IQDRGYVKIK KNKFYAEKMG EILTIRLKKS FSNLIDYNFT AHMEKKLDQV 550
AENKVTWRYL LDDFFKKFSE QLEQAKKSPE EGGMELNNIV PTSLNCPICC 600
KKMGIKTAIT GVFLSCLGYN NTDNKKRCKK TINLITLNDF NKEQDNQKKI 650
SLQLIQKCDI CNMYMDSYFI NEKLKLHICA NNPSCSGYKF EKGVFKSPIY 700
LSKTIQCEKC YNNMKLKIGP FGKFFTCINK ICKNTRKILP NGEISDPKLE 750
PIPFPELLCK QSDAWFVLRE GISGIFFAAN TFPKSRETRS PFVEELVRFQ 800
HLLPEKIYYL SSAPVIDNYG NKTIVCFDKK KKTHYIASKK DGKFTGWSAV 850
FIDQKWCVIN K 861
Length:861
Mass (Da):99,740
Last modified:December 1, 2000 - v1
Checksum:i903BBF2EA5265599
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12994.1.
RefSeqiNP_240108.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12994; BAB12994; BAB12994.
GeneIDi1109983.
KEGGibuc:BU284.
PATRICi21244092. VBIBucAph127364_0294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12994.1 .
RefSeqi NP_240108.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57371.
SMRi P57371. Positions 2-569, 741-856.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12994 ; BAB12994 ; BAB12994 .
GeneIDi 1109983.
KEGGi buc:BU284.
PATRICi 21244092. VBIBucAph127364_0294.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000004018.
KOi K03168.
OMAi VVECDKC.
OrthoDBi EOG6S7XQ9.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-284-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiTOP1_BUCAI
AccessioniPrimary (citable) accession number: P57371
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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