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P57371 (TOP1_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene names
Name:topA
Ordered Locus Names:BU284
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length861 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00952

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00952

Cofactor

Magnesium. Binds two Mg2+ per subunit By similarity. HAMAP-Rule MF_00952

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00952

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 861861DNA topoisomerase 1 HAMAP-Rule MF_00952
PRO_0000145144

Regions

Domain3 – 141139Toprim
Zinc finger596 – 62833C4-type 1 HAMAP-Rule MF_00952
Zinc finger658 – 68528C4-type 2 HAMAP-Rule MF_00952
Zinc finger707 – 73226C4-type 3 HAMAP-Rule MF_00952
Region191 – 1966Interaction with DNA By similarity

Sites

Active site3181O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding91Magnesium 1; catalytic By similarity
Metal binding1101Magnesium 1; catalytic By similarity
Metal binding1101Magnesium 2 By similarity
Metal binding1121Magnesium 2 By similarity
Site331Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site1681Interaction with DNA By similarity
Site1711Interaction with DNA By similarity
Site1761Interaction with DNA By similarity
Site1831Interaction with DNA By similarity
Site3201Interaction with DNA By similarity
Site5041Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P57371 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 903BBF2EA5265599

FASTA86199,740
        10         20         30         40         50         60 
MQKSLVIVES PAKAKTINQY LGSEYIVKSS IGHIRDLTKG KLYNKEKNKK FLNENFIEKT 

        70         80         90        100        110        120 
NENKILIKQM GIDPYQNWKF EYHILPGKEK IISELKYIAN QVKHIYLATD LDREGEAIAW 

       130        140        150        160        170        180 
HLKEVIGGDS SKFSRVVFNE ITQHSIQKAF KNVGHINMNR VHAQQARRFM DRIVGYMISP 

       190        200        210        220        230        240 
LLWKKIARGL SAGRVQSVAV RIIADRESII KNFVPEEYWK LDVSLISQDK KKINMDVTHY 

       250        260        270        280        290        300 
NNKKFRPINE NEVSFAVEKI QKSSCIVKNY EEKISYLKAP APFITSTLQQ SASLRLGFSV 

       310        320        330        340        350        360 
KKTMFLAQKL YEEGYITYMR TDSNYLSEYA IKKVRKYIKS NYGSNYLPKE PNIYSNEKHS 

       370        380        390        400        410        420 
QEAHEAIRPS DIKIKNIDSD HLNSSAKKLY ELIWNQFLAS QMKSVKYKSI TVTVLADMFK 

       430        440        450        460        470        480 
LQKSERIVMF QGWNKVLIEE KNVFSQFPIL QTGSQLFINK VTPSQKFTKP PPRFSEASLV 

       490        500        510        520        530        540 
RELEKKGIGR PSTYSAITSK IQDRGYVKIK KNKFYAEKMG EILTIRLKKS FSNLIDYNFT 

       550        560        570        580        590        600 
AHMEKKLDQV AENKVTWRYL LDDFFKKFSE QLEQAKKSPE EGGMELNNIV PTSLNCPICC 

       610        620        630        640        650        660 
KKMGIKTAIT GVFLSCLGYN NTDNKKRCKK TINLITLNDF NKEQDNQKKI SLQLIQKCDI 

       670        680        690        700        710        720 
CNMYMDSYFI NEKLKLHICA NNPSCSGYKF EKGVFKSPIY LSKTIQCEKC YNNMKLKIGP 

       730        740        750        760        770        780 
FGKFFTCINK ICKNTRKILP NGEISDPKLE PIPFPELLCK QSDAWFVLRE GISGIFFAAN 

       790        800        810        820        830        840 
TFPKSRETRS PFVEELVRFQ HLLPEKIYYL SSAPVIDNYG NKTIVCFDKK KKTHYIASKK 

       850        860 
DGKFTGWSAV FIDQKWCVIN K 

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References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12994.1.
RefSeqNP_240108.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57371.
SMRP57371. Positions 2-569, 741-856.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12994; BAB12994; BAB12994.
GeneID1109983.
KEGGbuc:BU284.
PATRIC21244092. VBIBucAph127364_0294.

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000004018.
KOK03168.
OMAVVECDKC.
OrthoDBEOG6S7XQ9.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-284-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPMF_00952. Topoisom_1_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR013263. TopoI_Znr_bac.
IPR028612. Topoisom_1_IA.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF08272. Topo_Zn_Ribbon. 2 hits.
PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01051. topA_bact. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_BUCAI
AccessionPrimary (citable) accession number: P57371
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names