P57368 (Y281_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized transporter BU281

Gene names

Ordered Locus Names:

BU281

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the EamA transporter family. Contains 2 EamA domains.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Uncharacterized transporter BU281		PRO_0000108189
Regions
	Transmembrane	4 – 24	21	Helical; Potential
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	120 – 140	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Transmembrane	214 – 234	21	Helical; Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Transmembrane	267 – 287	21	Helical; Potential
	Domain	15 – 139	125	EamA 1
	Domain	161 – 287	127	EamA 2

Sequences

	Sequence		Length	Mass (Da)
	P57368 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 5D1851AECF6EA6CE	FASTA	307	35,006
	10 20 30 40 50 60 MNKILVIILF SLVSLTWGTT WIAMKIATET IPPFFATGMR FLVASPILII LAYFTKTPLL 70 80 90 100 110 120 FPYGQRWFQL IISIFYFSIP FALMLYGGMY VSSSVASIIF SSMPVAVLTV SFLYLKKKLF 130 140 150 160 170 180 LTQKIGMFIS LITLLTILLI ELESQCFFQW KGILALLFAL FSHAFIYSEC QKKCLNVSVI 190 200 210 220 230 240 TFNALPSLLS GILLSTTSWF IESPHINTFS NRSILAIFYL GDFSGIFGIL SYFYLQQKVS 250 260 270 280 290 300 AFYASTVFLI FPVIAGFLEN YIYKNAILLC EIWFIFPLII GILLTLIPVN YLKKIRNKIT RLSCLLF

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12991.1.
RefSeq	NP_240105.1. NC_002528.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12991; BAB12991; BAB12991.
GeneID	1109980.
KEGG	buc:BU281.
PATRIC	21244086. VBIBucAph127364_0291.
Phylogenomic databases
eggNOG	COG0697.
HOGENOM	HOG000219630.
KO	K07790.
OMA	ESPHINT.
ProtClustDB	CLSK862159.
Family and domain databases
InterPro	IPR000620. DMT. [Graphical view]
Pfam	PF00892. EamA. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y281_BUCAI

Accession

Primary (citable) accession number: P57368

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program