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P57365 (TRPA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan synthase alpha chain

EC=4.2.1.20
Gene names
Name:trpA
Ordered Locus Names:BU277
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length269 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate By similarity. HAMAP-Rule MF_00131

Catalytic activity

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00131

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00131

Subunit structure

Tetramer of two alpha and two beta chains By similarity.

Sequence similarities

Belongs to the TrpA family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Aromatic amino acid biosynthesis
Tryptophan biosynthesis
   Molecular functionLyase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Molecular_functiontryptophan synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 269269Tryptophan synthase alpha chain HAMAP-Rule MF_00131
PRO_0000098754

Sites

Active site491Proton acceptor By similarity
Active site601Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
P57365 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: B1A59668F09BF3A5

FASTA26930,323
        10         20         30         40         50         60 
MNRYQETFKR LSCLKEGCFI PFVVLGDPSL ETSIKIIETL IKSGADALEI GIPFSDPLAD 

        70         80         90        100        110        120 
GPTVQKSNLR ALSKNNTFFE YFKILKQLRK KNKKLPIGIL IYANLVYNQG IDNFYFQCFN 

       130        140        150        160        170        180 
SGLDSVLIAD VPIEESKIFY NIANKYKINP IFICPPDADA DFLYKISLYA QGFIYVLSRP 

       190        200        210        220        230        240 
GVTGIKNNTI ALPRDFINKI KKYNSVPLLQ GFGISNPKQI KEAISSGLSG VICGSAIINI 

       250        260 
IEKYLNQEKK MIKEIKKFTH LLKLSTKLE 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12987.1.
RefSeqNP_240101.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57365.
SMRP57365. Positions 2-267.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12987; BAB12987; BAB12987.
GeneID1109716.
KEGGbuc:BU277.
PATRIC21244078. VBIBucAph127364_0287.

Phylogenomic databases

eggNOGCOG0159.
HOGENOMHOG000223814.
KOK01695.
OMAATTEHVT.
OrthoDBEOG6RVG0K.
ProtClustDBPRK13111.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-277-MONOMER.
UniPathwayUPA00035; UER00044.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00131. Trp_synth_alpha.
InterProIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
TIGRFAMsTIGR00262. trpA. 1 hit.
PROSITEPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPA_BUCAI
AccessionPrimary (citable) accession number: P57365
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names