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P57353

- FABI_BUCAI

UniProt

P57353 - FABI_BUCAI

Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.By similarity

    Catalytic activityi

    An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei13 – 131NAD; via carbonyl oxygenBy similarity
    Binding sitei40 – 401NADBy similarity
    Binding sitei92 – 921NAD; via carbonyl oxygenBy similarity
    Active sitei146 – 1461Proton acceptorBy similarity
    Active sitei156 – 1561Proton acceptorBy similarity
    Binding sitei163 – 1631NADBy similarity
    Sitei204 – 2041Involved in acyl-ACP bindingBy similarity
    Sitei205 – 2051Involved in acyl-ACP bindingBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi19 – 202NADBy similarity
    Nucleotide bindingi64 – 652NADBy similarity
    Nucleotide bindingi192 – 1965NADBy similarity

    GO - Molecular functioni

    1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB
    2. fatty acid elongation Source: UniProtKB
    3. protein homotetramerization Source: UniProtKB

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-265-MONOMER.
    UniPathwayiUPA00078.
    UPA00094.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
    Short name:
    ENR
    Alternative name(s):
    NADH-dependent enoyl-ACP reductase
    Gene namesi
    Name:fabI
    Ordered Locus Names:BU265
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260Enoyl-[acyl-carrier-protein] reductase [NADH] FabIPRO_0000054896Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57353.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0623.
    KOiK00208.
    OMAiKLSIAWA.
    OrthoDBiEOG6HF644.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57353-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFLKGKKFL IIGISSVRSI AFGIAKSLYN QKAELGFVCQ NEKIRKKIKK    50
    LVEPMKSCIV LCCDVSNDKN IKKLFIDLGQ VWKKFDGLVH AIAYCPKQYF 100
    NGDFIDNITR EGFNISHEIS SYSFIGLVKA CRTMLNNFSS LLTLSYLGSQ 150
    RVVPNYNVMG LAKASLEASV RYMASSLGKE NIRVNAISSS PIKTISSYKI 200
    TNFNKIRNCS YASSFLKNSV TSENIGNVAS FLLSDLSLGI TGSVIYVDNG 250
    FNVSSMNNIQ 260
    Length:260
    Mass (Da):28,779
    Last modified:December 1, 2000 - v1
    Checksum:iDCA04AC740D6DADD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12975.1.
    RefSeqiNP_240089.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12975; BAB12975; BAB12975.
    GeneIDi1109704.
    KEGGibuc:BU265.
    PATRICi21244054. VBIBucAph127364_0275.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12975.1 .
    RefSeqi NP_240089.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57353.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12975 ; BAB12975 ; BAB12975 .
    GeneIDi 1109704.
    KEGGi buc:BU265.
    PATRICi 21244054. VBIBucAph127364_0275.

    Phylogenomic databases

    eggNOGi COG0623.
    KOi K00208.
    OMAi KLSIAWA.
    OrthoDBi EOG6HF644.

    Enzyme and pathway databases

    UniPathwayi UPA00078 .
    UPA00094 .
    BioCyci BAPH107806:GBZJ-265-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiFABI_BUCAI
    AccessioniPrimary (citable) accession number: P57353
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3