P57353 (FABI_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Short name=ENR
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	BU265

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis. Cofactor biosynthesis; biotin biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Biotin biosynthesis Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW fatty acid elongation Inferred from sequence or structural similarity. Source: UniProtKB protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from sequence or structural similarity. Source: UniProtKB nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Enoyl-[acyl-carrier-protein] reductase [NADH] FabI		PRO_0000054896
Regions
	Nucleotide binding	19 – 20	2	NAD By similarity
	Nucleotide binding	64 – 65	2	NAD By similarity
	Nucleotide binding	192 – 194	3	NAD By similarity
Sites
	Active site	146	1	Proton acceptor By similarity
	Active site	156	1	Proton acceptor By similarity
	Binding site	13	1	NAD; via carbonyl oxygen By similarity
	Binding site	40	1	NAD By similarity
	Binding site	92	1	NAD; via carbonyl oxygen By similarity
	Binding site	163	1	NAD By similarity
	Site	204	1	Involved in acyl-ACP binding By similarity
	Site	205	1	Involved in acyl-ACP binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57353 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: DCA04AC740D6DADD	FASTA	260	28,779
	10 20 30 40 50 60 MGFLKGKKFL IIGISSVRSI AFGIAKSLYN QKAELGFVCQ NEKIRKKIKK LVEPMKSCIV 70 80 90 100 110 120 LCCDVSNDKN IKKLFIDLGQ VWKKFDGLVH AIAYCPKQYF NGDFIDNITR EGFNISHEIS 130 140 150 160 170 180 SYSFIGLVKA CRTMLNNFSS LLTLSYLGSQ RVVPNYNVMG LAKASLEASV RYMASSLGKE 190 200 210 220 230 240 NIRVNAISSS PIKTISSYKI TNFNKIRNCS YASSFLKNSV TSENIGNVAS FLLSDLSLGI 250 260 TGSVIYVDNG FNVSSMNNIQ

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12975.1.
RefSeq	NP_240089.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57353.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003298; EBBUCP00000003074; EBBUCG00000003298.
GeneID	1109704.
GenomeReviews	Gene locus BU265 in contig BA000003_GR.
KEGG	buc:BU265.
PATRIC	21244054. VBIBucAph127364_0275.
Phylogenomic databases
GeneTree	EBGT00050000007854.
HOGENOM	HBG750976.
OMA	DCDVGSD.
PhylomeDB	P57353.
ProtClustDB	CLSK315843.
Enzyme and pathway databases
BioCyc	BSP107806:BU265-MONOMER.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00208.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PIRSF	PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABI_BUCAI

Accession

Primary (citable) accession number: P57353

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program