Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Enoyl-[acyl-carrier-protein] reductase [NADH] FabI

Gene

fabI

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis (By similarity).By similarity

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi: fatty acid biosynthesis

This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei13NAD; via carbonyl oxygenBy similarity1
Binding sitei40NADBy similarity1
Binding sitei92NAD; via carbonyl oxygenBy similarity1
Active sitei146Proton acceptorBy similarity1
Active sitei156Proton acceptorBy similarity1
Binding sitei163NADBy similarity1
Sitei204Involved in acyl-ACP bindingBy similarity1
Sitei205Involved in acyl-ACP bindingBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 20NADBy similarity2
Nucleotide bindingi64 – 65NADBy similarity2
Nucleotide bindingi192 – 196NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00078.
UPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:BU265
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000548961 – 260Enoyl-[acyl-carrier-protein] reductase [NADH] FabIAdd BLAST260

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi107806.BU265.

Structurei

3D structure databases

ProteinModelPortaliP57353.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
KOiK00208.
OMAiSACKREG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P57353-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLKGKKFL IIGISSVRSI AFGIAKSLYN QKAELGFVCQ NEKIRKKIKK
60 70 80 90 100
LVEPMKSCIV LCCDVSNDKN IKKLFIDLGQ VWKKFDGLVH AIAYCPKQYF
110 120 130 140 150
NGDFIDNITR EGFNISHEIS SYSFIGLVKA CRTMLNNFSS LLTLSYLGSQ
160 170 180 190 200
RVVPNYNVMG LAKASLEASV RYMASSLGKE NIRVNAISSS PIKTISSYKI
210 220 230 240 250
TNFNKIRNCS YASSFLKNSV TSENIGNVAS FLLSDLSLGI TGSVIYVDNG
260
FNVSSMNNIQ
Length:260
Mass (Da):28,779
Last modified:December 1, 2000 - v1
Checksum:iDCA04AC740D6DADD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12975.1.
RefSeqiNP_240089.1. NC_002528.1.
WP_010896033.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12975; BAB12975; BAB12975.
GeneIDi1109704.
KEGGibuc:BU265.
PATRICi21244054. VBIBucAph127364_0275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12975.1.
RefSeqiNP_240089.1. NC_002528.1.
WP_010896033.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57353.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU265.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12975; BAB12975; BAB12975.
GeneIDi1109704.
KEGGibuc:BU265.
PATRICi21244054. VBIBucAph127364_0275.

Phylogenomic databases

eggNOGiENOG4105CSJ. Bacteria.
COG0623. LUCA.
KOiK00208.
OMAiSACKREG.

Enzyme and pathway databases

UniPathwayiUPA00078.
UPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PTHR24322:SF317. PTHR24322:SF317. 2 hits.
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFABI_BUCAI
AccessioniPrimary (citable) accession number: P57353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.