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P57353

- FABI_BUCAI

UniProt

P57353 - FABI_BUCAI

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Protein
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Gene
fabI, BU265
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP). Involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis By similarity.

Catalytic activityi

An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131NAD; via carbonyl oxygen By similarity
Binding sitei40 – 401NAD By similarity
Binding sitei92 – 921NAD; via carbonyl oxygen By similarity
Active sitei146 – 1461Proton acceptor By similarity
Active sitei156 – 1561Proton acceptor By similarity
Binding sitei163 – 1631NAD By similarity
Sitei204 – 2041Involved in acyl-ACP binding By similarity
Sitei205 – 2051Involved in acyl-ACP binding By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 202NAD By similarity
Nucleotide bindingi64 – 652NAD By similarity
Nucleotide bindingi192 – 1965NAD By similarity

GO - Molecular functioni

  1. enoyl-[acyl-carrier-protein] reductase (NADH) activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB
  2. fatty acid elongation Source: UniProtKB
  3. protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Biotin biosynthesis, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-265-MONOMER.
UniPathwayiUPA00078.
UPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH] FabI (EC:1.3.1.9)
Short name:
ENR
Alternative name(s):
NADH-dependent enoyl-ACP reductase
Gene namesi
Name:fabI
Ordered Locus Names:BU265
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
PRO_0000054896Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57353.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0623.
KOiK00208.
OMAiKLSIAWA.
OrthoDBiEOG6HF644.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PIRSFiPIRSF000094. Enoyl-ACP_rdct. 1 hit.

Sequencei

Sequence statusi: Complete.

P57353-1 [UniParc]FASTAAdd to Basket

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MGFLKGKKFL IIGISSVRSI AFGIAKSLYN QKAELGFVCQ NEKIRKKIKK    50
LVEPMKSCIV LCCDVSNDKN IKKLFIDLGQ VWKKFDGLVH AIAYCPKQYF 100
NGDFIDNITR EGFNISHEIS SYSFIGLVKA CRTMLNNFSS LLTLSYLGSQ 150
RVVPNYNVMG LAKASLEASV RYMASSLGKE NIRVNAISSS PIKTISSYKI 200
TNFNKIRNCS YASSFLKNSV TSENIGNVAS FLLSDLSLGI TGSVIYVDNG 250
FNVSSMNNIQ 260
Length:260
Mass (Da):28,779
Last modified:December 1, 2000 - v1
Checksum:iDCA04AC740D6DADD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12975.1.
RefSeqiNP_240089.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12975; BAB12975; BAB12975.
GeneIDi1109704.
KEGGibuc:BU265.
PATRICi21244054. VBIBucAph127364_0275.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12975.1 .
RefSeqi NP_240089.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57353.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12975 ; BAB12975 ; BAB12975 .
GeneIDi 1109704.
KEGGi buc:BU265.
PATRICi 21244054. VBIBucAph127364_0275.

Phylogenomic databases

eggNOGi COG0623.
KOi K00208.
OMAi KLSIAWA.
OrthoDBi EOG6HF644.

Enzyme and pathway databases

UniPathwayi UPA00078 .
UPA00094 .
BioCyci BAPH107806:GBZJ-265-MONOMER.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR014358. Enoyl-ACP_Rdtase_NADH.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PIRSFi PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiFABI_BUCAI
AccessioniPrimary (citable) accession number: P57353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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