Reviewed, UniProtKB/Swiss-Prot P57353 (FABI_BUCAI)

Names and origin

Protein names

Recommended name:
    Enoyl-[acyl-carrier-protein] reductase [NADH]
    EC=1.3.1.9
Alternative name(s):
    NADH-dependent enoyl-ACP reductase

Gene names

Name:	fabI
Ordered Locus Names:	BU265

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054896
Regions
	Nucleotide binding	10 – 36	27	NAD By similarity
Sites
	Active site	156	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57353-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: DCA04AC740D6DADD	FASTA	260	28,779
	10 20 30 40 50 60 MGFLKGKKFL IIGISSVRSI AFGIAKSLYN QKAELGFVCQ NEKIRKKIKK LVEPMKSCIV 70 80 90 100 110 120 LCCDVSNDKN IKKLFIDLGQ VWKKFDGLVH AIAYCPKQYF NGDFIDNITR EGFNISHEIS 130 140 150 160 170 180 SYSFIGLVKA CRTMLNNFSS LLTLSYLGSQ RVVPNYNVMG LAKASLEASV RYMASSLGKE 190 200 210 220 230 240 NIRVNAISSS PIKTISSYKI TNFNKIRNCS YASSFLKNSV TSENIGNVAS FLLSDLSLGI 250 260 TGSVIYVDNG FNVSSMNNIQ

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12975.1.
RefSeq	NP_240089.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFI based on UniProtKB P29132.
ModBase	Search...
Genome annotation databases
GeneID	1109704.
GenomeReviews	Gene locus BU265 in contig BA000003_GR.
KEGG	buc:BU265.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57353.
OMA	P57353. ECDVGSD.
Enzyme and pathway databases
BioCyc	BSP107806:BU265-MON.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR014358. Enoyl-ACP_Rdtase_NADH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit. PTHR19410:SF12. Enoyl-ACP_rdct. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABI_BUCAI

Accession

Primary (citable) accession number: P57353

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)