Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor 4

Gene

lepA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi27 – 32GTPUniRule annotation6
Nucleotide bindingi144 – 147GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:BU260
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesErwiniaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001762461 – 610Elongation factor 4Add BLAST610

Interactioni

Protein-protein interaction databases

STRINGi107806.BU260

Structurei

3D structure databases

ProteinModelPortaliP57348
SMRiP57348
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 197tr-type GAdd BLAST183

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4S Bacteria
COG0481 LUCA
HOGENOMiHOG000020624
KOiK03596
OMAiALIFDSW

Family and domain databases

CDDicd03709 lepA_C, 1 hit
Gene3Di3.30.70.25701 hit
HAMAPiMF_00071 LepA, 1 hit
InterProiView protein in InterPro
IPR006297 EF-4
IPR035647 EFG_III/V
IPR000640 EFG_V-like
IPR004161 EFTu-like_2
IPR031157 G_TR_CS
IPR038363 LepA_C_sf
IPR013842 LepA_CTD
IPR035654 LepA_IV
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
PfamiView protein in Pfam
PF00679 EFG_C, 1 hit
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF06421 LepA_C, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF52540 SSF52540, 1 hit
SSF54980 SSF54980, 2 hits
TIGRFAMsiTIGR01393 lepA, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P57348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLKCIKHEYG IKNMKSIRNF SIIAHIDHGK STLSDRLIQL CGGLSEREMS
60 70 80 90 100
NQVLDSMDLE KERGITIKAQ SVMIDYKNKS GNIFNLNFID TPGHVDFSYE
110 120 130 140 150
VSRSLAACEG ALLVVDSTQG VEAQTLANCY TAIDMNVEIV PVLNKIDLPN
160 170 180 190 200
SNADKVAKEI EDIIGIPALD AIRCSAKTGE GIEDLIERII NDIPYPKGSI
210 220 230 240 250
NSPLQALIID SWFDNYLGVV SLIRIKNGIL FERDKIQVMS TGKNYYVDQI
260 270 280 290 300
GVFTPKKLNK NQLRCGEVGW IICGIKNIIA APVGDTLTTA KNPAKNMIIG
310 320 330 340 350
FKKIKPQIYA GLFPLTSDQY EMFRDALGKL SLNDASLFYE PENSVALGFG
360 370 380 390 400
FRCGFLGVLH MEIIQARLER EYSIDLITTI PTVIYEIELI NGKIIYLDTP
410 420 430 440 450
SNFPNMNDIK IIKEPIVECS ILSPPQFLGS IIKLCIKKRG VQINMVYHSH
460 470 480 490 500
QVLLKYNIPM NEVILNFFDE LKSVSSGYAS LEYDFKYFQS VKMVRIDILI
510 520 530 540 550
NSEKVDALTI LSYHKNAQSR SREIVDKMKK LIPRHQFDIS IQAVINNSVI
560 570 580 590 600
ARSTIKQLRK NVLSKCYGGD VSRKKKLLQK QKDGKKRMKK IGNVNVPKTV
610
FLSILNSRES
Length:610
Mass (Da):68,635
Last modified:December 1, 2000 - v1
Checksum:i4DD2A82427F04FDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA Translation: BAB12970.1
RefSeqiNP_240084.1, NC_002528.1
WP_010896030.1, NC_002528.1

Genome annotation databases

EnsemblBacteriaiBAB12970; BAB12970; BAB12970
GeneIDi1109699
KEGGibuc:BU260
PATRICifig|107806.10.peg.270

Similar proteinsi

Entry informationi

Entry nameiLEPA_BUCAI
AccessioniPrimary (citable) accession number: P57348
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 28, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome