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P57348 (LEPA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Elongation factor 4

Short name=EF-4
EC=3.6.5.n1
Alternative name(s):
Ribosomal back-translocase LepA
Gene names
Name:lepA
Ordered Locus Names:BU260
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length610 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071

Catalytic activity

GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071

Subcellular location

Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.

Sequence similarities

Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCell membrane
Membrane
   LigandGTP-binding
Nucleotide-binding
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of translation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionGTP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

GTPase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

ribosome binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

translation elongation factor activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 610610Elongation factor 4 HAMAP-Rule MF_00071
PRO_0000176246

Regions

Nucleotide binding27 – 326GTP By similarity
Nucleotide binding144 – 1474GTP By similarity

Sequences

Sequence LengthMass (Da)Tools
P57348 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 4DD2A82427F04FDA

FASTA61068,635
        10         20         30         40         50         60 
MLKCIKHEYG IKNMKSIRNF SIIAHIDHGK STLSDRLIQL CGGLSEREMS NQVLDSMDLE 

        70         80         90        100        110        120 
KERGITIKAQ SVMIDYKNKS GNIFNLNFID TPGHVDFSYE VSRSLAACEG ALLVVDSTQG 

       130        140        150        160        170        180 
VEAQTLANCY TAIDMNVEIV PVLNKIDLPN SNADKVAKEI EDIIGIPALD AIRCSAKTGE 

       190        200        210        220        230        240 
GIEDLIERII NDIPYPKGSI NSPLQALIID SWFDNYLGVV SLIRIKNGIL FERDKIQVMS 

       250        260        270        280        290        300 
TGKNYYVDQI GVFTPKKLNK NQLRCGEVGW IICGIKNIIA APVGDTLTTA KNPAKNMIIG 

       310        320        330        340        350        360 
FKKIKPQIYA GLFPLTSDQY EMFRDALGKL SLNDASLFYE PENSVALGFG FRCGFLGVLH 

       370        380        390        400        410        420 
MEIIQARLER EYSIDLITTI PTVIYEIELI NGKIIYLDTP SNFPNMNDIK IIKEPIVECS 

       430        440        450        460        470        480 
ILSPPQFLGS IIKLCIKKRG VQINMVYHSH QVLLKYNIPM NEVILNFFDE LKSVSSGYAS 

       490        500        510        520        530        540 
LEYDFKYFQS VKMVRIDILI NSEKVDALTI LSYHKNAQSR SREIVDKMKK LIPRHQFDIS 

       550        560        570        580        590        600 
IQAVINNSVI ARSTIKQLRK NVLSKCYGGD VSRKKKLLQK QKDGKKRMKK IGNVNVPKTV 

       610 
FLSILNSRES 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12970.1.
RefSeqNP_240084.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57348.
SMRP57348. Positions 14-568.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12970; BAB12970; BAB12970.
GeneID1109699.
KEGGbuc:BU260.
PATRIC21244044. VBIBucAph127364_0270.

Phylogenomic databases

eggNOGCOG0481.
HOGENOMHOG000020624.
KOK03596.
OMAKPMVFCG.
OrthoDBEOG6ZKXQ4.
ProtClustDBPRK05433.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-260-MONOMER.

Family and domain databases

Gene3D3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00071. LepA.
InterProIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSPR00315. ELONGATNFCT.
SMARTSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEPS00301. EFACTOR_GTP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLEPA_BUCAI
AccessionPrimary (citable) accession number: P57348
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: April 16, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names