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P57348

- LEPA_BUCAI

UniProt

P57348 - LEPA_BUCAI

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Protein

Elongation factor 4

Gene

lepA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

Catalytic activityi

GTP + H2O = GDP + phosphate.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi27 – 326GTPUniRule annotation
Nucleotide bindingi144 – 1474GTPUniRule annotation

GO - Molecular functioni

  1. GTPase activity Source: UniProtKB-HAMAP
  2. GTP binding Source: UniProtKB-HAMAP
  3. ribosome binding Source: UniProtKB-HAMAP
  4. translation elongation factor activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. positive regulation of translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-260-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
Short name:
EF-4UniRule annotation
Alternative name(s):
Ribosomal back-translocase LepAUniRule annotation
Gene namesi
Name:lepAUniRule annotation
Ordered Locus Names:BU260
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

GO - Cellular componenti

  1. plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 610610Elongation factor 4PRO_0000176246Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP57348.
SMRiP57348. Positions 14-568.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 197183tr-type GAdd
BLAST

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0481.
HOGENOMiHOG000020624.
KOiK03596.
OMAiCYTALEM.
OrthoDBiEOG6ZKXQ4.

Family and domain databases

Gene3Di3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00071. LepA.
InterProiIPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57348-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLKCIKHEYG IKNMKSIRNF SIIAHIDHGK STLSDRLIQL CGGLSEREMS
60 70 80 90 100
NQVLDSMDLE KERGITIKAQ SVMIDYKNKS GNIFNLNFID TPGHVDFSYE
110 120 130 140 150
VSRSLAACEG ALLVVDSTQG VEAQTLANCY TAIDMNVEIV PVLNKIDLPN
160 170 180 190 200
SNADKVAKEI EDIIGIPALD AIRCSAKTGE GIEDLIERII NDIPYPKGSI
210 220 230 240 250
NSPLQALIID SWFDNYLGVV SLIRIKNGIL FERDKIQVMS TGKNYYVDQI
260 270 280 290 300
GVFTPKKLNK NQLRCGEVGW IICGIKNIIA APVGDTLTTA KNPAKNMIIG
310 320 330 340 350
FKKIKPQIYA GLFPLTSDQY EMFRDALGKL SLNDASLFYE PENSVALGFG
360 370 380 390 400
FRCGFLGVLH MEIIQARLER EYSIDLITTI PTVIYEIELI NGKIIYLDTP
410 420 430 440 450
SNFPNMNDIK IIKEPIVECS ILSPPQFLGS IIKLCIKKRG VQINMVYHSH
460 470 480 490 500
QVLLKYNIPM NEVILNFFDE LKSVSSGYAS LEYDFKYFQS VKMVRIDILI
510 520 530 540 550
NSEKVDALTI LSYHKNAQSR SREIVDKMKK LIPRHQFDIS IQAVINNSVI
560 570 580 590 600
ARSTIKQLRK NVLSKCYGGD VSRKKKLLQK QKDGKKRMKK IGNVNVPKTV
610
FLSILNSRES
Length:610
Mass (Da):68,635
Last modified:December 1, 2000 - v1
Checksum:i4DD2A82427F04FDA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12970.1.
RefSeqiNP_240084.1. NC_002528.1.
WP_010896030.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12970; BAB12970; BAB12970.
GeneIDi1109699.
KEGGibuc:BU260.
PATRICi21244044. VBIBucAph127364_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12970.1 .
RefSeqi NP_240084.1. NC_002528.1.
WP_010896030.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57348.
SMRi P57348. Positions 14-568.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12970 ; BAB12970 ; BAB12970 .
GeneIDi 1109699.
KEGGi buc:BU260.
PATRICi 21244044. VBIBucAph127364_0270.

Phylogenomic databases

eggNOGi COG0481.
HOGENOMi HOG000020624.
KOi K03596.
OMAi CYTALEM.
OrthoDBi EOG6ZKXQ4.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-260-MONOMER.

Family and domain databases

Gene3Di 3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00071. LepA.
InterProi IPR006297. EF-4.
IPR000795. EF_GTP-bd_dom.
IPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR013842. LepA_GTP-bd_C.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR004161. Transl_elong_EFTu/EF1A_2.
[Graphical view ]
Pfami PF00679. EFG_C. 1 hit.
PF00009. GTP_EFTU. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
PF06421. LepA_C. 1 hit.
[Graphical view ]
PRINTSi PR00315. ELONGATNFCT.
SMARTi SM00838. EFG_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsi TIGR01393. lepA. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEi PS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiLEPA_BUCAI
AccessioniPrimary (citable) accession number: P57348
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3