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P57348

- LEPA_BUCAI

UniProt

P57348 - LEPA_BUCAI

Protein

Elongation factor 4

Gene

lepA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner.UniRule annotation

    Catalytic activityi

    GTP + H2O = GDP + phosphate.UniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi27 – 326GTPUniRule annotation
    Nucleotide bindingi144 – 1474GTPUniRule annotation

    GO - Molecular functioni

    1. GTPase activity Source: UniProtKB-HAMAP
    2. GTP binding Source: UniProtKB-HAMAP
    3. ribosome binding Source: UniProtKB-HAMAP
    4. translation elongation factor activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. positive regulation of translation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-260-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Elongation factor 4UniRule annotation (EC:3.6.5.n1UniRule annotation)
    Short name:
    EF-4UniRule annotation
    Alternative name(s):
    Ribosomal back-translocase LepAUniRule annotation
    Gene namesi
    Name:lepAUniRule annotation
    Ordered Locus Names:BU260
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation; Cytoplasmic side UniRule annotation

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 610610Elongation factor 4PRO_0000176246Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP57348.
    SMRiP57348. Positions 14-568.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 197183tr-type GAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. LepA subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0481.
    HOGENOMiHOG000020624.
    KOiK03596.
    OMAiCYTALEM.
    OrthoDBiEOG6ZKXQ4.

    Family and domain databases

    Gene3Di3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00071. LepA.
    InterProiIPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view]
    PfamiPF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view]
    PRINTSiPR00315. ELONGATNFCT.
    SMARTiSM00838. EFG_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsiTIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEiPS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57348-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLKCIKHEYG IKNMKSIRNF SIIAHIDHGK STLSDRLIQL CGGLSEREMS    50
    NQVLDSMDLE KERGITIKAQ SVMIDYKNKS GNIFNLNFID TPGHVDFSYE 100
    VSRSLAACEG ALLVVDSTQG VEAQTLANCY TAIDMNVEIV PVLNKIDLPN 150
    SNADKVAKEI EDIIGIPALD AIRCSAKTGE GIEDLIERII NDIPYPKGSI 200
    NSPLQALIID SWFDNYLGVV SLIRIKNGIL FERDKIQVMS TGKNYYVDQI 250
    GVFTPKKLNK NQLRCGEVGW IICGIKNIIA APVGDTLTTA KNPAKNMIIG 300
    FKKIKPQIYA GLFPLTSDQY EMFRDALGKL SLNDASLFYE PENSVALGFG 350
    FRCGFLGVLH MEIIQARLER EYSIDLITTI PTVIYEIELI NGKIIYLDTP 400
    SNFPNMNDIK IIKEPIVECS ILSPPQFLGS IIKLCIKKRG VQINMVYHSH 450
    QVLLKYNIPM NEVILNFFDE LKSVSSGYAS LEYDFKYFQS VKMVRIDILI 500
    NSEKVDALTI LSYHKNAQSR SREIVDKMKK LIPRHQFDIS IQAVINNSVI 550
    ARSTIKQLRK NVLSKCYGGD VSRKKKLLQK QKDGKKRMKK IGNVNVPKTV 600
    FLSILNSRES 610
    Length:610
    Mass (Da):68,635
    Last modified:December 1, 2000 - v1
    Checksum:i4DD2A82427F04FDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12970.1.
    RefSeqiNP_240084.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12970; BAB12970; BAB12970.
    GeneIDi1109699.
    KEGGibuc:BU260.
    PATRICi21244044. VBIBucAph127364_0270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12970.1 .
    RefSeqi NP_240084.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57348.
    SMRi P57348. Positions 14-568.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12970 ; BAB12970 ; BAB12970 .
    GeneIDi 1109699.
    KEGGi buc:BU260.
    PATRICi 21244044. VBIBucAph127364_0270.

    Phylogenomic databases

    eggNOGi COG0481.
    HOGENOMi HOG000020624.
    KOi K03596.
    OMAi CYTALEM.
    OrthoDBi EOG6ZKXQ4.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-260-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.240. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00071. LepA.
    InterProi IPR006297. EF-4.
    IPR000795. EF_GTP-bd_dom.
    IPR009022. EFG_III-V.
    IPR000640. EFG_V.
    IPR013842. LepA_GTP-bd_C.
    IPR027417. P-loop_NTPase.
    IPR005225. Small_GTP-bd_dom.
    IPR004161. Transl_elong_EFTu/EF1A_2.
    [Graphical view ]
    Pfami PF00679. EFG_C. 1 hit.
    PF00009. GTP_EFTU. 1 hit.
    PF03144. GTP_EFTU_D2. 1 hit.
    PF06421. LepA_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00315. ELONGATNFCT.
    SMARTi SM00838. EFG_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF54980. SSF54980. 2 hits.
    TIGRFAMsi TIGR01393. lepA. 1 hit.
    TIGR00231. small_GTP. 1 hit.
    PROSITEi PS00301. G_TR_1. 1 hit.
    PS51722. G_TR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiLEPA_BUCAI
    AccessioniPrimary (citable) accession number: P57348
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3