P57348 (LEPA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor 4
Short name=EF-4
EC=3.6.5.n1

Alternative name(s):
Ribosomal back-translocase LepA

Gene names

Name:	lepA
Ordered Locus Names:	BU260

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	610 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back-translocation proceeds from a post-translocation (POST) complex to a pre-translocation (PRE) complex, thus giving elongation factor G a second chance to translocate the tRNAs correctly. Binds to ribosomes in a GTP-dependent manner By similarity. HAMAP-Rule MF_00071
Catalytic activity	GTP + H2O = GDP + phosphate. HAMAP-Rule MF_00071
Subcellular location	Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity HAMAP-Rule MF_00071.
Sequence similarities	Belongs to the GTP-binding elongation factor family. LepA subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	GTP catabolic process Inferred from electronic annotation. Source: GOC positive regulation of translation Inferred from electronic annotation. Source: HAMAP translational elongation Inferred from electronic annotation. Source: GOC
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP ribosome binding Inferred from electronic annotation. Source: HAMAP translation elongation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 610	610	Elongation factor 4 HAMAP-Rule MF_00071		PRO_0000176246
Regions
	Nucleotide binding	27 – 32	6	GTP By similarity
	Nucleotide binding	144 – 147	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57348 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 4DD2A82427F04FDA	FASTA	610	68,635
	10 20 30 40 50 60 MLKCIKHEYG IKNMKSIRNF SIIAHIDHGK STLSDRLIQL CGGLSEREMS NQVLDSMDLE 70 80 90 100 110 120 KERGITIKAQ SVMIDYKNKS GNIFNLNFID TPGHVDFSYE VSRSLAACEG ALLVVDSTQG 130 140 150 160 170 180 VEAQTLANCY TAIDMNVEIV PVLNKIDLPN SNADKVAKEI EDIIGIPALD AIRCSAKTGE 190 200 210 220 230 240 GIEDLIERII NDIPYPKGSI NSPLQALIID SWFDNYLGVV SLIRIKNGIL FERDKIQVMS 250 260 270 280 290 300 TGKNYYVDQI GVFTPKKLNK NQLRCGEVGW IICGIKNIIA APVGDTLTTA KNPAKNMIIG 310 320 330 340 350 360 FKKIKPQIYA GLFPLTSDQY EMFRDALGKL SLNDASLFYE PENSVALGFG FRCGFLGVLH 370 380 390 400 410 420 MEIIQARLER EYSIDLITTI PTVIYEIELI NGKIIYLDTP SNFPNMNDIK IIKEPIVECS 430 440 450 460 470 480 ILSPPQFLGS IIKLCIKKRG VQINMVYHSH QVLLKYNIPM NEVILNFFDE LKSVSSGYAS 490 500 510 520 530 540 LEYDFKYFQS VKMVRIDILI NSEKVDALTI LSYHKNAQSR SREIVDKMKK LIPRHQFDIS 550 560 570 580 590 600 IQAVINNSVI ARSTIKQLRK NVLSKCYGGD VSRKKKLLQK QKDGKKRMKK IGNVNVPKTV 610 FLSILNSRES

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12970.1.
RefSeq	NP_240084.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57348.
SMR	P57348. Positions 14-568.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12970; BAB12970; BAB12970.
GeneID	1109699.
KEGG	buc:BU260.
PATRIC	21244044. VBIBucAph127364_0270.
Phylogenomic databases
eggNOG	COG0481.
HOGENOM	HOG000020624.
KO	K03596.
OMA	KCYGGDA.
ProtClustDB	PRK05433.
Family and domain databases
Gene3D	3.30.70.240. 1 hit.
HAMAP	MF_00071. LepA.
InterPro	IPR006297. EF-4. IPR000795. EF_GTP-bd_dom. IPR009022. EFG_III-V. IPR000640. EFG_V. IPR013842. LepA_GTP-bd_C. IPR005225. Small_GTP-bd_dom. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR23115:SF40. PTHR23115:SF40. 1 hit.
Pfam	PF00679. EFG_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. PF06421. LepA_C. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR01393. lepA. 1 hit. TIGR00231. small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEPA_BUCAI

Accession

Primary (citable) accession number: P57348

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program