P57347 (LEP_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	lepB
Ordered Locus Names:	BU259

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Signal peptidase I		PRO_0000109503
Regions
	Transmembrane	5 – 25	21	Helical; Potential
	Topological domain	26 – 63	38	Cytoplasmic Potential
	Transmembrane	64 – 84	21	Helical; Potential
	Topological domain	85 – 314	230	Extracellular Potential
Sites
	Active site	88	1	By similarity
	Active site	143	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57347 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: B968E83705840780	FASTA	314	37,023
	10 20 30 40 50 60 MANILTIFLL ISTLVTGIFW SFYCIKSFKN YLINKKIINN NNFHQEKIEK SKNKTYFLKS 70 80 90 100 110 120 LASFFPIFLA IFIIRSFIYE PFQIPSGSMM PTLLVGDFIL VEKFSYGIKE PITHKILIRT 130 140 150 160 170 180 KKPNRGDIAV FQHPTDHNIN YIKRIIGLPG DKIRYDLHDK HIHICTNYSD QRGCEKKISI 190 200 210 220 230 240 NYSQSRSSNF IQKIYFSNKN NIKEDKNIYN SLYFDIVEEI IEDVKHSILL LNSIKNTKEN 250 260 270 280 290 300 YFQQKNMPKL TWIVPKGEYF MMGDNRDNSL DSRYWGFVPE KNLVGKAIKI WMSFDKNENE 310 WPTGIRINRI GSIH

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12969.1.
RefSeq	NP_240083.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57347.
SMR	P57347. Positions 75-314.
ModBase	Search...
Protein family/group databases
MEROPS	S26.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002806; EBBUCP00000002582; EBBUCG00000002806.
GeneID	1109698.
GenomeReviews	Gene locus BU259 in contig BA000003_GR.
KEGG	buc:BU259.
PATRIC	21244042. VBIBucAph127364_0269.
Phylogenomic databases
GeneTree	EBGT00050000007850.
HOGENOM	HBG596607.
OMA	PTGVRFE.
PhylomeDB	P57347.
ProtClustDB	CLSK315834.
Enzyme and pathway databases
BioCyc	BSP107806:BU259-MONOMER.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR019533. Peptidase_S26. IPR019766. Peptidase_S26A_all-beta_subdom. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits. G3DSA:2.170.230.10. Peptidase_S26A_all-beta_subdom. 1 hit.
KO	K03100.
Pfam	PF00717. Peptidase_S24. 1 hit. PF10502. Peptidase_S26. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_BUCAI

Accession

Primary (citable) accession number: P57347

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program