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P57338 (GMHA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoheptose isomerase
EC=5.3.1.28
Alternative name(s):
Sedoheptulose 7-phosphate isomerase
Gene names
Name:gmhA
Synonyms:lpcA
Ordered Locus Names:BU250
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera ›

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate By similarity. HAMAP-Rule MF_00067

Catalytic activity

D-sedoheptulose 7-phosphate = D-glycero-D-manno-heptose 7-phosphate. HAMAP-Rule MF_00067

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1. HAMAP-Rule MF_00067

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Miscellaneous

The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate By similarity. HAMAP-Rule MF_00067

B.aphidicola is not able to make lipopolysaccharides (LPS), and except lpcA and kdtB, the genes responsible for the biosynthesis of the LPS components are missing from the genome. HAMAP-Rule MF_00067

Sequence similarities

Belongs to the SIS family. GmhA subfamily.

Contains 1 SIS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 193193Phosphoheptose isomerase HAMAP-Rule MF_00067
PRO_0000136518

Regions

Domain37 – 193157SIS
Region52 – 543Substrate binding By similarity
Region93 – 942Substrate binding By similarity
Region119 – 1213Substrate binding By similarity

Sites

Metal binding611Zinc By similarity
Metal binding651Zinc By similarity
Metal binding1721Zinc By similarity
Metal binding1801Zinc By similarity
Binding site651Substrate By similarity
Binding site1241Substrate By similarity
Binding site1721Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P57338 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 7DB396B8DDF736E8

FASTA19321,610
        10         20         30         40         50         60 
MYKKIIFSEF NSASKILKNF LEDKKQIENI QKAAILIAQS FKNEKKVISC GNGGSHCDAV 

        70         80         90        100        110        120 
HFSEELTSVY RKKRSGYPAI SISDSSYISA VGNDFGYDQI FSRFIQSVGH LGDILLAIST 

       130        140        150        160        170        180 
SGNSLNIVRA IEEAKKKKMK VIVLTGNNAG KIKNLSDIEI CIPHCGYSDR IQEMHIKIIH 

       190 
ILILIIEKEM QKN

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB12960.1.
RefSeqNP_240074.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57338.
SMRP57338. Positions 1-192.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12960; BAB12960; BAB12960.
GeneID1109689.
KEGGbuc:BU250.
PATRIC21244024. VBIBucAph127364_0260.

Phylogenomic databases

eggNOGCOG0279.
HOGENOMHOG000237571.
KOK03271.
OMAGSMCDAM.
ProtClustDBPRK00414.

Enzyme and pathway databases

UniPathwayUPA00041; UER00436.

Family and domain databases

HAMAPMF_00067. GmhA.
InterProIPR004515. Phosphoheptose_Isoase.
IPR001347. SIS.
[Graphical view]
PfamPF13580. SIS_2. 1 hit.
[Graphical view]
TIGRFAMsTIGR00441. gmhA. 1 hit.
PROSITEPS51464. SIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGMHA_BUCAI
AccessionPrimary (citable) accession number: P57338
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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