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P57337

- DPO3E_BUCAI

UniProt

P57337 - DPO3E_BUCAI

Protein

DNA polymerase III subunit epsilon

Gene

dnaQ

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease By similarity.By similarity

    Catalytic activityi

    Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

    Cofactori

    Binds 2 divalent metal cations. Magnesium or manganese By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi10 – 101Divalent metal cation 1; catalyticBy similarity
    Metal bindingi10 – 101Divalent metal cation 2; catalyticBy similarity
    Binding sitei10 – 101SubstrateBy similarity
    Metal bindingi12 – 121Divalent metal cation 1; catalyticBy similarity
    Binding sitei12 – 121SubstrateBy similarity
    Binding sitei64 – 641SubstrateBy similarity
    Active sitei159 – 1591Proton acceptorBy similarity
    Metal bindingi164 – 1641Divalent metal cation 1; catalyticBy similarity
    Binding sitei164 – 1641SubstrateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
    3. exonuclease activity Source: UniProtKB-KW
    4. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-248-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA polymerase III subunit epsilon (EC:2.7.7.7)
    Gene namesi
    Name:dnaQ
    Ordered Locus Names:BU248
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237DNA polymerase III subunit epsilonPRO_0000105480Add
    BLAST

    Interactioni

    Subunit structurei

    The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP57337.
    SMRiP57337. Positions 5-177.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Phylogenomic databases

    eggNOGiCOG0847.
    HOGENOMiHOG000258616.
    KOiK02342.
    OMAiGFMDHEF.
    OrthoDBiEOG696BTR.

    Family and domain databases

    Gene3Di3.30.420.10. 1 hit.
    InterProiIPR006054. DnaQ.
    IPR006309. DnaQ_proteo.
    IPR006055. Exonuclease.
    IPR013520. Exonuclease_RNaseT/DNA_pol3.
    IPR012337. RNaseH-like_dom.
    [Graphical view]
    PfamiPF00929. RNase_T. 1 hit.
    [Graphical view]
    SMARTiSM00479. EXOIII. 1 hit.
    [Graphical view]
    SUPFAMiSSF53098. SSF53098. 1 hit.
    TIGRFAMsiTIGR00573. dnaq. 1 hit.
    TIGR01406. dnaQ_proteo. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57337-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRKRTIILD TETTGINQTS LPHINHRIIE IGAVEIIDRC FTGNNFHVYI    50
    QPGRSIESGA LKVHGITNKF LLDKPIFKDI ADSFLNYIKN SILVIHNASF 100
    DVGFINQELE ILNKKIKINT FCSIIDTLKI ARELFPGKKN TLDALCTRYK 150
    INKSHRNLHS AIVDSYLLGK LYLLMTGGQD SLFSDNTINY KENFKKLKKN 200
    IQLKNNTLRI LHPTLKENDL HEKYLQYMKD KSTCLWN 237
    Length:237
    Mass (Da):27,365
    Last modified:December 1, 2000 - v1
    Checksum:i3E4C4F102C50EBB7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12959.1.
    RefSeqiNP_240073.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12959; BAB12959; BAB12959.
    GeneIDi1109688.
    KEGGibuc:BU248.
    PATRICi21244020. VBIBucAph127364_0259.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12959.1 .
    RefSeqi NP_240073.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57337.
    SMRi P57337. Positions 5-177.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12959 ; BAB12959 ; BAB12959 .
    GeneIDi 1109688.
    KEGGi buc:BU248.
    PATRICi 21244020. VBIBucAph127364_0259.

    Phylogenomic databases

    eggNOGi COG0847.
    HOGENOMi HOG000258616.
    KOi K02342.
    OMAi GFMDHEF.
    OrthoDBi EOG696BTR.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-248-MONOMER.

    Family and domain databases

    Gene3Di 3.30.420.10. 1 hit.
    InterProi IPR006054. DnaQ.
    IPR006309. DnaQ_proteo.
    IPR006055. Exonuclease.
    IPR013520. Exonuclease_RNaseT/DNA_pol3.
    IPR012337. RNaseH-like_dom.
    [Graphical view ]
    Pfami PF00929. RNase_T. 1 hit.
    [Graphical view ]
    SMARTi SM00479. EXOIII. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53098. SSF53098. 1 hit.
    TIGRFAMsi TIGR00573. dnaq. 1 hit.
    TIGR01406. dnaQ_proteo. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiDPO3E_BUCAI
    AccessioniPrimary (citable) accession number: P57337
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

    External Data

    Dasty 3