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P57337

- DPO3E_BUCAI

UniProt

P57337 - DPO3E_BUCAI

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Protein
DNA polymerase III subunit epsilon
Gene
dnaQ, BU248
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. The epsilon subunit contain the editing function and is a proofreading 3'-5' exonuclease By similarity.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

Cofactori

Binds 2 divalent metal cations. Magnesium or manganese By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Divalent metal cation 1; catalytic By similarity
Metal bindingi10 – 101Divalent metal cation 2; catalytic By similarity
Binding sitei10 – 101Substrate By similarity
Metal bindingi12 – 121Divalent metal cation 1; catalytic By similarity
Binding sitei12 – 121Substrate By similarity
Binding sitei64 – 641Substrate By similarity
Active sitei159 – 1591Proton acceptor By similarity
Metal bindingi164 – 1641Divalent metal cation 1; catalytic By similarity
Binding sitei164 – 1641Substrate By similarity

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA-directed DNA polymerase activity Source: UniProtKB-KW
  3. exonuclease activity Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Magnesium, Manganese, Metal-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-248-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase III subunit epsilon (EC:2.7.7.7)
Gene namesi
Name:dnaQ
Ordered Locus Names:BU248
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237DNA polymerase III subunit epsilon
PRO_0000105480Add
BLAST

Interactioni

Subunit structurei

The DNA polymerase holoenzyme is a complex that contains 10 different types of subunits. These subunits are organized into 3 functionally essential subassemblies: the pol III core, the beta sliding clamp processivity factor and the clamp-loading complex. The pol III core (subunits alpha,epsilon and theta) contains the polymerase and the 3'-5' exonuclease proofreading activities. The polymerase is tethered to the template via the sliding clamp processivity factor. The clamp-loading complex assembles the beta processivity factor onto the primer template and plays a central role in the organization and communication at the replication fork. This complex contains delta, delta', psi and chi, and copies of either or both of two different DnaX proteins, gamma and tau. The composition of the holoenzyme is, therefore: (alpha,epsilon,theta)[2]-(gamma/tau)[3]-delta,delta', psi,chi-beta[4] By similarity.

Structurei

3D structure databases

ProteinModelPortaliP57337.
SMRiP57337. Positions 5-177.

Family & Domainsi

Phylogenomic databases

eggNOGiCOG0847.
HOGENOMiHOG000258616.
KOiK02342.
OMAiGFMDHEF.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
InterProiIPR006054. DnaQ.
IPR006309. DnaQ_proteo.
IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view]
PfamiPF00929. RNase_T. 1 hit.
[Graphical view]
SMARTiSM00479. EXOIII. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00573. dnaq. 1 hit.
TIGR01406. dnaQ_proteo. 1 hit.

Sequencei

Sequence statusi: Complete.

P57337-1 [UniParc]FASTAAdd to Basket

« Hide

MNRKRTIILD TETTGINQTS LPHINHRIIE IGAVEIIDRC FTGNNFHVYI    50
QPGRSIESGA LKVHGITNKF LLDKPIFKDI ADSFLNYIKN SILVIHNASF 100
DVGFINQELE ILNKKIKINT FCSIIDTLKI ARELFPGKKN TLDALCTRYK 150
INKSHRNLHS AIVDSYLLGK LYLLMTGGQD SLFSDNTINY KENFKKLKKN 200
IQLKNNTLRI LHPTLKENDL HEKYLQYMKD KSTCLWN 237
Length:237
Mass (Da):27,365
Last modified:December 1, 2000 - v1
Checksum:i3E4C4F102C50EBB7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12959.1.
RefSeqiNP_240073.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12959; BAB12959; BAB12959.
GeneIDi1109688.
KEGGibuc:BU248.
PATRICi21244020. VBIBucAph127364_0259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12959.1 .
RefSeqi NP_240073.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57337.
SMRi P57337. Positions 5-177.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12959 ; BAB12959 ; BAB12959 .
GeneIDi 1109688.
KEGGi buc:BU248.
PATRICi 21244020. VBIBucAph127364_0259.

Phylogenomic databases

eggNOGi COG0847.
HOGENOMi HOG000258616.
KOi K02342.
OMAi GFMDHEF.
OrthoDBi EOG696BTR.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-248-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
InterProi IPR006054. DnaQ.
IPR006309. DnaQ_proteo.
IPR006055. Exonuclease.
IPR013520. Exonuclease_RNaseT/DNA_pol3.
IPR012337. RNaseH-like_dom.
[Graphical view ]
Pfami PF00929. RNase_T. 1 hit.
[Graphical view ]
SMARTi SM00479. EXOIII. 1 hit.
[Graphical view ]
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00573. dnaq. 1 hit.
TIGR01406. dnaQ_proteo. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiDPO3E_BUCAI
AccessioniPrimary (citable) accession number: P57337
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

External Data

Dasty 3

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