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Reviewed, UniProtKB/Swiss-Prot P57336 (GLO2_BUCAI)

Last modified February 9, 2010. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Hydroxyacylglutathione hydrolase
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II
Gene names
Name: gloB
Ordered Locus Names: BU246
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]
Taxonomic identifier118099 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

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Protein attributes

Sequence length251 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374

Catalytic activity

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374

Cofactor

Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374

Pathway

Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374

Subunit structure

Monomer By similarity. HAMAP MF_01374

Sequence similarities

Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

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Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular functionhydroxyacylglutathione hydrolase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Hydroxyacylglutathione hydrolase HAMAP MF_01374
PRO_0000192348

Sites

Metal binding531Zinc 1 By similarity
Metal binding551Zinc 1 By similarity
Metal binding571Zinc 2 By similarity
Metal binding581Zinc 2 By similarity
Metal binding1101Zinc 1 By similarity
Metal binding1271Zinc 1 By similarity
Metal binding1271Zinc 2 By similarity
Metal binding1651Zinc 2 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P57336-1 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: D023179CAFF715CF

FASTA25129,156
        10         20         30         40         50         60 
MILKKISILS DNYVWVLLNT SGSCIIIDPG LSEPIIQEIE RKKWRLRAIL LTHNHIDHTG 

        70         80         90        100        110        120 
GTRKIIEYFP KISVFGPKET RQHGVNKIVS HGDRIILLDK IFYVFFTPGH TSGHVSYYSQ 

       130        140        150        160        170        180 
PYIFCGDTLF SAGCGRVFKN KHLEMYRSIK IISSLPDSTL LCCSHEYTLS NLQFSMFILP 

       190        200        210        220        230        240 
NDNFIKLYLK KIEIKLKLGQ SSLPSYIFFE KKINLFLRTN DNYVKKSIGL KSTCTDFEVF 

       250 
KRLRLKKDFW S

« Hide

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References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed: 10993077] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Tokyo 1998.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000003 Genomic DNA. Translation: BAB12958.1.
RefSeqNP_240072.1.

3D structure databases

SMRP57336. Positions 1-250.
ModBaseSearch...

Genome annotation databases

GeneID1109686.
GenomeReviewsGene locus BU246 in contig BA000003_GR.
KEGGbuc:BU246.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG753931.
OMAWCAHEYT.
PhylomeDBP57336.

Enzyme and pathway databases

BioCycBSP107806:BU246-MONOMER.

Family and domain databases

HAMAPMF_01374. Glyoxalase_2.
[Tree]
InterProIPR001279. Blactmase-like.
IPR017782. Hydroxyacylglutathione_Hdrlase.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
TIGRFAMsTIGR03413. GSH_gloB. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGLO2_BUCAI
AccessionPrimary (citable) accession number: P57336
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 9, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents