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P57333

- SYP_BUCAI

UniProt

P57333 - SYP_BUCAI

Protein

Proline--tRNA ligase

Gene

proS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

    Catalytic activityi

    ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. proline-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. prolyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-239-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
    Alternative name(s):
    Prolyl-tRNA synthetaseUniRule annotation
    Short name:
    ProRSUniRule annotation
    Gene namesi
    Name:proSUniRule annotation
    Ordered Locus Names:BU239
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 572572Proline--tRNA ligasePRO_0000139322Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57333.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0442.
    HOGENOMiHOG000076893.
    KOiK01881.
    OMAiMHQAYCN.
    OrthoDBiEOG6TTVMR.

    Family and domain databases

    Gene3Di3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPiMF_01569. Pro_tRNA_synth_type1.
    InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view]
    PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
    PfamiPF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view]
    PRINTSiPR01046. TRNASYNTHPRO.
    SUPFAMiSSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
    PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P57333-1 [UniParc]FASTAAdd to Basket

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    MLTSQYLLST SKDIPYDAKI ISHQLMIRSG MIRKTSSGLY VWLPTGMRVL    50
    KKIKNIITTE MEKINALEIL MPIIQPEYLW KESRRLNLYG EELLRFLDRR 100
    KNQFILGPTN EEVVTNFIGS EIHSYKQLPL TVYQIQTKFR DEIRPRFGII 150
    RTREFTMKDA YSFHINQSCL ENTYNKFYDS YINIFKKMNL NFCAVKADSG 200
    SMGGNISHEF QAFSQNGEDE IVFSNDKLYS SNMNMAESIE TIDFFKKKYS 250
    SCLIKNKTNT KKSIIMSEKL NTPLINQIQT FLIQTKINDI TSIAALLIRG 300
    DHELNFFKVE KIDIINKPLV FLNEKEVISL IGVKKEFLGP LGLKVPIIAD 350
    ISTFNMKNFT IGSNINKHFF INVNWNIDLP MPIFKDIRKV TKNDLSPNGS 400
    GYLNIKQSIE IGHIFQLGQK YSRKIQQSVK IKNGNLKNLY MGCYGIGITR 450
    IAAAVIEQHH DKNGIIWPDS IAPFEVVILP INMKKDNKIK IIAHFLYKKF 500
    KKTGIDVILD DRDERPGVMF NEVDLIGIPH QIIISKRSIN YDNVEYRERK 550
    NKENILINIK DIKNFIIQKL KK 572
    Length:572
    Mass (Da):66,325
    Last modified:December 1, 2000 - v1
    Checksum:i44B4BF1A73F16E68
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12954.1.
    RefSeqiNP_240068.1. NC_002528.1.
    WP_010896022.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12954; BAB12954; BAB12954.
    GeneIDi1109682.
    KEGGibuc:BU239.
    PATRICi21244000. VBIBucAph127364_0252.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12954.1 .
    RefSeqi NP_240068.1. NC_002528.1.
    WP_010896022.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57333.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12954 ; BAB12954 ; BAB12954 .
    GeneIDi 1109682.
    KEGGi buc:BU239.
    PATRICi 21244000. VBIBucAph127364_0252.

    Phylogenomic databases

    eggNOGi COG0442.
    HOGENOMi HOG000076893.
    KOi K01881.
    OMAi MHQAYCN.
    OrthoDBi EOG6TTVMR.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-239-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.800. 1 hit.
    3.90.960.10. 1 hit.
    HAMAPi MF_01569. Pro_tRNA_synth_type1.
    InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
    IPR006195. aa-tRNA-synth_II.
    IPR004154. Anticodon-bd.
    IPR002316. Pro-tRNA-ligase_IIa.
    IPR004500. Pro-tRNA-synth_IIa_bac-type.
    IPR023717. Pro-tRNA-Synthase_IIa_type1.
    IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
    [Graphical view ]
    PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
    Pfami PF03129. HGTP_anticodon. 1 hit.
    PF00587. tRNA-synt_2b. 1 hit.
    PF04073. tRNA_edit. 1 hit.
    [Graphical view ]
    PRINTSi PR01046. TRNASYNTHPRO.
    SUPFAMi SSF52954. SSF52954. 1 hit.
    SSF55826. SSF55826. 1 hit.
    TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
    PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiSYP_BUCAI
    AccessioniPrimary (citable) accession number: P57333
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3