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Protein

Proline--tRNA ligase

Gene

proS

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-239-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligaseUniRule annotation (EC:6.1.1.15UniRule annotation)
Alternative name(s):
Prolyl-tRNA synthetaseUniRule annotation
Short name:
ProRSUniRule annotation
Gene namesi
Name:proSUniRule annotation
Ordered Locus Names:BU239
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Proline--tRNA ligasePRO_0000139322Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi107806.BU239.

Structurei

3D structure databases

ProteinModelPortaliP57333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57333-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTSQYLLST SKDIPYDAKI ISHQLMIRSG MIRKTSSGLY VWLPTGMRVL
60 70 80 90 100
KKIKNIITTE MEKINALEIL MPIIQPEYLW KESRRLNLYG EELLRFLDRR
110 120 130 140 150
KNQFILGPTN EEVVTNFIGS EIHSYKQLPL TVYQIQTKFR DEIRPRFGII
160 170 180 190 200
RTREFTMKDA YSFHINQSCL ENTYNKFYDS YINIFKKMNL NFCAVKADSG
210 220 230 240 250
SMGGNISHEF QAFSQNGEDE IVFSNDKLYS SNMNMAESIE TIDFFKKKYS
260 270 280 290 300
SCLIKNKTNT KKSIIMSEKL NTPLINQIQT FLIQTKINDI TSIAALLIRG
310 320 330 340 350
DHELNFFKVE KIDIINKPLV FLNEKEVISL IGVKKEFLGP LGLKVPIIAD
360 370 380 390 400
ISTFNMKNFT IGSNINKHFF INVNWNIDLP MPIFKDIRKV TKNDLSPNGS
410 420 430 440 450
GYLNIKQSIE IGHIFQLGQK YSRKIQQSVK IKNGNLKNLY MGCYGIGITR
460 470 480 490 500
IAAAVIEQHH DKNGIIWPDS IAPFEVVILP INMKKDNKIK IIAHFLYKKF
510 520 530 540 550
KKTGIDVILD DRDERPGVMF NEVDLIGIPH QIIISKRSIN YDNVEYRERK
560 570
NKENILINIK DIKNFIIQKL KK
Length:572
Mass (Da):66,325
Last modified:December 1, 2000 - v1
Checksum:i44B4BF1A73F16E68
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12954.1.
RefSeqiNP_240068.1. NC_002528.1.
WP_010896022.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12954; BAB12954; BAB12954.
GeneIDi1109682.
KEGGibuc:BU239.
PATRICi21244000. VBIBucAph127364_0252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12954.1.
RefSeqiNP_240068.1. NC_002528.1.
WP_010896022.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU239.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12954; BAB12954; BAB12954.
GeneIDi1109682.
KEGGibuc:BU239.
PATRICi21244000. VBIBucAph127364_0252.

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076893.
KOiK01881.
OMAiIQPAELW.
OrthoDBiEOG6TTVMR.

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-239-MONOMER.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiSYP_BUCAI
AccessioniPrimary (citable) accession number: P57333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 24, 2015
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.