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P57333

- SYP_BUCAI

UniProt

P57333 - SYP_BUCAI

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Protein
Proline--tRNA ligase
Gene
proS, BU239
Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.UniRule annotation

Catalytic activityi

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).UniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. proline-tRNA ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. prolyl-tRNA aminoacylation Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-239-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Proline--tRNA ligase (EC:6.1.1.15)
Alternative name(s):
Prolyl-tRNA synthetase
Short name:
ProRS
Gene namesi
Name:proS
Ordered Locus Names:BU239
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000001806: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 572572Proline--tRNA ligaseUniRule annotation
PRO_0000139322Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP57333.

Family & Domainsi

Domaini

Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0442.
HOGENOMiHOG000076893.
KOiK01881.
OMAiMHQAYCN.
OrthoDBiEOG6TTVMR.

Family and domain databases

Gene3Di3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPiMF_01569. Pro_tRNA_synth_type1.
InterProiIPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view]
PANTHERiPTHR11451:SF3. PTHR11451:SF3. 1 hit.
PfamiPF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view]
PRINTSiPR01046. TRNASYNTHPRO.
SUPFAMiSSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsiTIGR00409. proS_fam_II. 1 hit.
PROSITEiPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P57333-1 [UniParc]FASTAAdd to Basket

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MLTSQYLLST SKDIPYDAKI ISHQLMIRSG MIRKTSSGLY VWLPTGMRVL    50
KKIKNIITTE MEKINALEIL MPIIQPEYLW KESRRLNLYG EELLRFLDRR 100
KNQFILGPTN EEVVTNFIGS EIHSYKQLPL TVYQIQTKFR DEIRPRFGII 150
RTREFTMKDA YSFHINQSCL ENTYNKFYDS YINIFKKMNL NFCAVKADSG 200
SMGGNISHEF QAFSQNGEDE IVFSNDKLYS SNMNMAESIE TIDFFKKKYS 250
SCLIKNKTNT KKSIIMSEKL NTPLINQIQT FLIQTKINDI TSIAALLIRG 300
DHELNFFKVE KIDIINKPLV FLNEKEVISL IGVKKEFLGP LGLKVPIIAD 350
ISTFNMKNFT IGSNINKHFF INVNWNIDLP MPIFKDIRKV TKNDLSPNGS 400
GYLNIKQSIE IGHIFQLGQK YSRKIQQSVK IKNGNLKNLY MGCYGIGITR 450
IAAAVIEQHH DKNGIIWPDS IAPFEVVILP INMKKDNKIK IIAHFLYKKF 500
KKTGIDVILD DRDERPGVMF NEVDLIGIPH QIIISKRSIN YDNVEYRERK 550
NKENILINIK DIKNFIIQKL KK 572
Length:572
Mass (Da):66,325
Last modified:December 1, 2000 - v1
Checksum:i44B4BF1A73F16E68
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12954.1.
RefSeqiNP_240068.1. NC_002528.1.
WP_010896022.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12954; BAB12954; BAB12954.
GeneIDi1109682.
KEGGibuc:BU239.
PATRICi21244000. VBIBucAph127364_0252.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000003 Genomic DNA. Translation: BAB12954.1 .
RefSeqi NP_240068.1. NC_002528.1.
WP_010896022.1. NC_002528.1.

3D structure databases

ProteinModelPortali P57333.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB12954 ; BAB12954 ; BAB12954 .
GeneIDi 1109682.
KEGGi buc:BU239.
PATRICi 21244000. VBIBucAph127364_0252.

Phylogenomic databases

eggNOGi COG0442.
HOGENOMi HOG000076893.
KOi K01881.
OMAi MHQAYCN.
OrthoDBi EOG6TTVMR.

Enzyme and pathway databases

BioCyci BAPH107806:GBZJ-239-MONOMER.

Family and domain databases

Gene3Di 3.40.50.800. 1 hit.
3.90.960.10. 1 hit.
HAMAPi MF_01569. Pro_tRNA_synth_type1.
InterProi IPR002314. aa-tRNA-synt_IIb_cons-dom.
IPR006195. aa-tRNA-synth_II.
IPR004154. Anticodon-bd.
IPR002316. Pro-tRNA-ligase_IIa.
IPR004500. Pro-tRNA-synth_IIa_bac-type.
IPR023717. Pro-tRNA-Synthase_IIa_type1.
IPR007214. YbaK/aa-tRNA-synth-assoc-dom.
[Graphical view ]
PANTHERi PTHR11451:SF3. PTHR11451:SF3. 1 hit.
Pfami PF03129. HGTP_anticodon. 1 hit.
PF00587. tRNA-synt_2b. 1 hit.
PF04073. tRNA_edit. 1 hit.
[Graphical view ]
PRINTSi PR01046. TRNASYNTHPRO.
SUPFAMi SSF52954. SSF52954. 1 hit.
SSF55826. SSF55826. 1 hit.
TIGRFAMsi TIGR00409. proS_fam_II. 1 hit.
PROSITEi PS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiSYP_BUCAI
AccessioniPrimary (citable) accession number: P57333
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: September 3, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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