Reviewed, UniProtKB/Swiss-Prot P57324 (AMPM_BUCAI)

Names and origin

Protein names

Recommended name:
    Methionine aminopeptidase
      Short name=MAP
    EC=3.4.11.18
Alternative name(s):
    Peptidase M

Gene names

Name:	map
Ordered Locus Names:	BU230

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins By similarity.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity. Binds 1 sodium ion per subunit. The sodium ion has a structural role By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Methionine aminopeptidase		PRO_0000148929
Sites
	Metal binding	97	1	Cobalt 1 By similarity
	Metal binding	108	1	Cobalt 1 By similarity
	Metal binding	108	1	Cobalt 2 By similarity
	Metal binding	171	1	Cobalt 2 By similarity
	Metal binding	204	1	Cobalt 2 By similarity
	Metal binding	235	1	Cobalt 1 By similarity
	Metal binding	235	1	Cobalt 2 By similarity
	Binding site	79	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57324-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 4FCCFAE8AC9FFF3D	FASTA	264	30,012
	10 20 30 40 50 60 MSCIIKTESE IKKMRISGKL AAEVLEMIKE HLQPKISTED INQICHDYIV YKKKAISACL 70 80 90 100 110 120 GYHGFPKSIC ISINDVVCHG IPSKNQVFKE GDIVNIDIAI IKDGYHGDTS KMFYIGKTSI 130 140 150 160 170 180 LSKRLCQVAR ESLYLSLKLV KPGIPLYKIG EIIQNYVESN NFSVVKEYCG HGIGRNFHEE 190 200 210 220 230 240 PHVLHYKNKK NNIILKKGMI FTIEPMINSG NPEVKCMKDG WTVKTKDRSL SAQYEHTVLV 250 260 TEYGCDILTW QKDEDISQKL VNIN

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12945.1.
RefSeq	NP_240059.1.
3D structure databases
SMR	P57324. Positions 5-256.
ModBase	Search...
Protein family/group databases
MEROPS	M24.001.
Genome annotation databases
GeneID	1109673.
GenomeReviews	Gene locus BU230 in contig BA000003_GR.
KEGG	buc:BU230.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG299384.
OMA	VIQKHAE.
PhylomeDB	P57324.
Enzyme and pathway databases
BioCyc	BSP107806:BU230-MONOMER.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
PANTHER	PTHR10804:SF13. Pept_M24A_MAP1. 1 hit. PTHR10804. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
TIGRFAMs	TIGR00500. met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_BUCAI

Accession

Primary (citable) accession number: P57324

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)