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P57315 (MURF_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase

EC=6.3.2.10
Alternative name(s):
D-alanyl-D-alanine-adding enzyme
UDP-MurNAc-pentapeptide synthetase
Gene names
Name:murF
Ordered Locus Names:BU220
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length455 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein By similarity.

Catalytic activity

ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subunit structure

Monomer By similarity.

Subcellular location

Cytoplasm Probable.

Sequence similarities

Belongs to the MurCDEF family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 455455UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
PRO_0000101696

Regions

Nucleotide binding107 – 1137ATP Potential

Sequences

Sequence LengthMass (Da)Tools
P57315 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: E292D8E9290E67B7

FASTA45550,452
        10         20         30         40         50         60 
MISLSLKKIA LITNGTLYGA DLLINEIVID TKKIIPGCLF IALIGRKFDA HIFIHDALKK 

        70         80         90        100        110        120 
KCAAFVTQKN IKPHVPYIIV ENTSIALGQI AGWVRKKTKA KILAITGSCG KTSVKEMTAS 

       130        140        150        160        170        180 
ILRKNGNTIS TIDNLNNNIG VPMTLLQLKQ EHKYGVIELG ASKPGEIAYT SNISQPDIIL 

       190        200        210        220        230        240 
INNIHCAHLQ GFKSLLGVSK AKSEIFSGLK PNSTVIINLD SHHFSQWKKD IKNSNILFFS 

       250        260        270        280        290        300 
IKKKKYSNFF CSNIKIHIHG TSFTMHTPCG KINISLPFLG YQNISNALAA SAFSFALKIP 

       310        320        330        340        350        360 
LKKIKIGLLD TPIVSKRLES IILEPNKILI DDTYNSNVSS MISAIKVLER MPGYKILVTG 

       370        380        390        400        410        420 
DMAELGENSM MYHQMIGNTA NSSAINKIFS IGDMSSEITK IFNNGKHFLN KKKLSEYLKN 

       430        440        450 
VFLKKKKITI LVKGSRSTKM EKVVEDLIKE SKKKC 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12936.1.
RefSeqNP_240050.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57315.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12936; BAB12936; BAB12936.
GeneID1109663.
KEGGbuc:BU220.
PATRIC21243962. VBIBucAph127364_0233.

Phylogenomic databases

eggNOGCOG0770.
HOGENOMHOG000268120.
KOK01929.
OMAANHLGEI.
OrthoDBEOG6PKFCR.
ProtClustDBCLSK315800.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-219-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.1190.10. 1 hit.
3.40.1390.10. 1 hit.
3.90.190.20. 1 hit.
InterProIPR004101. Mur_ligase_C.
IPR013221. Mur_ligase_cen.
IPR000713. Mur_ligase_N.
IPR005863. UDP-N-AcMur-pentapeptide_synth.
[Graphical view]
PANTHERPTHR23135:SF3. PTHR23135:SF3. 1 hit.
PfamPF01225. Mur_ligase. 1 hit.
PF02875. Mur_ligase_C. 1 hit.
PF08245. Mur_ligase_M. 1 hit.
[Graphical view]
SUPFAMSSF53244. SSF53244. 1 hit.
SSF53623. SSF53623. 1 hit.
SSF63418. SSF63418. 1 hit.
TIGRFAMsTIGR01143. murF. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMURF_BUCAI
AccessionPrimary (citable) accession number: P57315
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: March 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names