Reviewed, UniProtKB/Swiss-Prot P57313 (MURD_BUCAI)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	BU218

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	440 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 440	440	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108984
Regions
	Nucleotide binding	113 – 119	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P57313-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: B343A7235FD9AE33	FASTA	440	49,529
	10 20 30 40 50 60 MSYNYFGKKI LILGMGLTGI SCINFFLKKG IKPKIIDESK HPSNFIKIPQ NIEYSLGSLD 70 80 90 100 110 120 HQWILESDLI VISPGISSFK PILIKARLLG IEIISDIELF SREVTCPIIS ITGTNGKSTV 130 140 150 160 170 180 ATMIEKIAKK SGYKAFLGGN IGVPVLEILD KEADLYIIEL SSFQLENTFN LKSKIAVILN 190 200 210 220 230 240 ISEDHINRYP NGFQQYKNTK LSVYNQAEIC IINSNDKIEK SLIHSKNKKW ISFGTNRSDY 250 260 270 280 290 300 RICSKSNDPI LFFKNKKILN TSEILLYGYH NYNNILVSLA ISDAMQFPRN DAINVLKSFS 310 320 330 340 350 360 NLPHRFQIIK NEKGVRWIND SKSTNVNSTQ VALNSIKTTG TIRLLLGGDS KSANFNILKN 370 380 390 400 410 420 IFRTLKIKIY CFGRDGIKLS KICEKKSIYV ENLKKAVILI SKQVKSGDTV LLSPGCSSLG 430 440 QFSNFEERGN LFIKLIKEIT

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12934.1.
RefSeq	NP_240048.1.
3D structure databases
HSSP	HSSP built from PDB template 1E0D based on UniProtKB P14900.
ModBase	Search...
Genome annotation databases
GeneID	1109661.
GenomeReviews	Gene locus BU218 in contig BA000003_GR.
KEGG	buc:BU218.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57313.
OMA	SEDHLDR.
Enzyme and pathway databases
BioCyc	BSP107806:BU218-MON.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_BUCAI

Accession

Primary (citable) accession number: P57313

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 3, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)