P57310 (MURC_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	BU215

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	484 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 484	484	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_0000182068
Regions
	Nucleotide binding	125 – 131	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P57310 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 06359D435D5590A7	FASTA	484	54,312
	10 20 30 40 50 60 MNIKDIKKIN FFISEKKNKN IHLIGIGGAG MMGIALILLK LGYKVSGSDL LESLMIKKLI 70 80 90 100 110 120 NLGATIYLQH SEKNIKNVDF IIKSSAISSN NKEILAAKKR NIPILLRAEM IEILMSFKKG 130 140 150 160 170 180 IAVSGTHGKT TTTSMIADIF IDSGLDPTVI NGGLIKSINS YAKLGSSSYF ITEADESDAS 190 200 210 220 230 240 FLYLNPNIII VTNIEPDHID HYDNSFKKLK QTFLIFLKKI TLYGTAIVCI DNNAICDILT 250 260 270 280 290 300 NLKCKIITYG FNKNADVRIF LYKQNNFIGH FYIILKNNKK LNIILNIPGK HNALNAAAAI 310 320 330 340 350 360 ALAIHEGINN DLMIASLKNF QGTCRRFEFL GFLSIDQGFD KRANCMLIDD YGHHPTELSE 370 380 390 400 410 420 TIKTIRISWP NKNLIMIFQP HRYTRTYNLY HDFVQTLSQV DVLLILHVYS ANEKFIVGAD 430 440 450 460 470 480 SLSLFNDIKK LGKNYVTLIS NHNMILDTLI QTLQGNDIIL IQGAGNIDTI AHKILIKKTK KVIK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12931.1.
RefSeq	NP_240045.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57310.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002953; EBBUCP00000002729; EBBUCG00000002953.
GeneID	1109658.
GenomeReviews	Gene locus BU215 in contig BA000003_GR.
KEGG	buc:BU215.
PATRIC	21243952. VBIBucAph127364_0228.
Phylogenomic databases
GeneTree	EBGT00050000007738.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	P57310.
ProtClustDB	CLSK2459963.
Enzyme and pathway databases
BioCyc	BSP107806:BU215-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_BUCAI

Accession

Primary (citable) accession number: P57310

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program