P57305 (SPEE_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine synthase
EC=2.5.1.16

Alternative name(s):
Putrescine aminopropyltransferase
Short name=PAPT
SPDSY

Gene names

Name:	speE
Ordered Locus Names:	BU209

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	286 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor By similarity. HAMAP MF_00198
Catalytic activity	S-adenosylmethioninamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine. HAMAP MF_00198
Pathway	Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1. HAMAP MF_00198
Subunit structure	Homodimer and homotetramer By similarity. HAMAP MF_00198
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	spermidine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	spermidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 286	286	Spermidine synthase HAMAP MF_00198		PRO_0000156472
Regions
	Region	140 – 141	2	S-adenosylmethioninamine binding By similarity
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	63	1	Putrescine By similarity
	Binding site	88	1	S-adenosylmethioninamine By similarity
	Binding site	108	1	S-adenosylmethioninamine By similarity
	Binding site	161	1	Putrescine By similarity
	Binding site	226	1	Putrescine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57305 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 5EE47D2107EB689E	FASTA	286	33,788
	10 20 30 40 50 60 MDHKKTWHEK LYCHLGQYFL IEKMLYKKKT PHHQVMIFKN SVFGKIMVID DIVQTTERDE 70 80 90 100 110 120 FIYHEMLTHV PIIAHGSIKS VLIIGGGDGG ILREVCRYKM IENITMVEID VNIIDLCKKY 130 140 150 160 170 180 FPNHSNQAYQ DSRLNLIIDD GLNFIKRTKE KFDLIISDST DPIGCGKNLF RSEFYFNCKN 190 200 210 220 230 240 HLEENGIFVA QNGVFFLQKN ETILTYKNLK KYFYDTRFYQ ANVPTYYGGV MVFAWGTNNI 250 260 270 280 EYRKNSLEKI QIRIKNTKLD FNYYNAKIHI SSFYLPQYIL NELNES

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12926.1.
RefSeq	NP_240040.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57305.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000003169; EBBUCP00000002945; EBBUCG00000003169.
GeneID	1109652.
GenomeReviews	Gene locus BU209 in contig BA000003_GR.
KEGG	buc:BU209.
PATRIC	21243938. VBIBucAph127364_0221.
Phylogenomic databases
GeneTree	EBGT00050000008010.
HOGENOM	HBG635113.
OMA	PCGHIGF.
PhylomeDB	P57305.
ProtClustDB	PRK00811.
Enzyme and pathway databases
BioCyc	BSP107806:BU209-MONOMER.
Family and domain databases
HAMAP	MF_00198. Spermidine_synth. [Tree]
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
KO	K00797.
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. SpeE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEE_BUCAI

Accession

Primary (citable) accession number: P57305

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program