P57301 (ODP1_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate dehydrogenase E1 component
Short name=PDH E1 component
EC=1.2.4.1

Gene names

Name:	aceE
Ordered Locus Names:	BU205

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Component of the pyruvate dehydrogenase (PDH) complex, that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2 By similarity.
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity. Makes part of the PDH complex, consisting of multiple copies of pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	Pyruvate dehydrogenase E1 component		PRO_0000162240

Sequences

	Sequence		Length	Mass (Da)
	P57301 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: B6AB82C012826105	FASTA	887	101,396
	10 20 30 40 50 60 MSENLYNDVD PIETRDWVQA IESVIRREGH KRAHFLIEQV LKTAKINRKE FFRSSFTSDY 70 80 90 100 110 120 INTISREDEY EYPGNLILEK RIRSAIRWNA IMMVLRASKK NLELGGHLSS FQSSATIYEV 130 140 150 160 170 180 CFNHFFQAKN HKDGGDLVYF QGHISPGIYA RSFLEGRLSE EQIDNFRQEV DGIGLSSYPH 190 200 210 220 230 240 PKLMPNFWQF PTVSMGLGPL CAIYQAKFLK YLHNRELKNT SKQIVYAFLG DGEMDEPESK 250 260 270 280 290 300 GAISIAVREK LDNLIFIINC NLQRLDGPVV GNGKIVNELE SFFYGAGWKV IKVIWGSRWD 310 320 330 340 350 360 CLLKKDTSGK LIQLMNETVD GDYQTFKSKD GAYVRKYFFG KYKETYDLVK DMTDEEIWKL 370 380 390 400 410 420 NRGGHDPKKM FNALKKAKET KYKPTVILAH TVKGYGMGVI AEGKNIAHQI KKININGIIH 430 440 450 460 470 480 IRDRFNIPVS NDEINKLPYV TFKKNSEEYC YIHSQRKKLG GYIPFRLSSF TGKLILPKLI 490 500 510 520 530 540 DFQSLLEEQK KDISTTVAFI RVLNIILKNN SIKHLIVPII ADEARTFGME GLFRKIGIYS 550 560 570 580 590 600 SSGQKYTPQD REQLAYYKEE KKGQILQEGI NELGAASSWL AAATSYSTND FPMILFYIYY 610 620 630 640 650 660 SIFGFQRIGD LFWAAGDQQA RGFLIGGTSG RTTLNGEGLQ HEDGHSHIQS LTIPNCISYD 670 680 690 700 710 720 PAFAYEVAVI IQDGLRRMYG PSQENIYYYI TTINENYYMP AMPIGVEEGI CKGIYKLKTL 730 740 750 760 770 780 HGTTSKVQLI GSGAILRSVC EAAEILLKDY SITTDIYSVT SFTELARNGE DCERWNMLHP 790 800 810 820 830 840 NEKNKIAYVK QIMNKNPTVA ATDYMKLFAE QIRHYIPSQE YHVLGTDGFG RSDSRDKLRD 850 860 870 880 HFEVNAYYIV IAALNLLANI NDIKKKVVED AIMKFNIDAN KINPRLS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12922.1.
RefSeq	NP_240036.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57301.
SMR	P57301. Positions 58-887.
ModBase	Search...
Proteomic databases
PRIDE	P57301.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12922; BAB12922; BAB12922.
GeneID	1109648.
KEGG	buc:BU205.
PATRIC	21243928. VBIBucAph127364_0216.
Phylogenomic databases
eggNOG	COG2609.
HOGENOM	HOG000115215.
KO	K00163.
OMA	NLIFTIS.
ProtClustDB	PRK09405.
Family and domain databases
Gene3D	3.40.50.920. 1 hit.
InterPro	IPR004660. 2-oxoA_DH_E1. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005474. Transketolase_N. [Graphical view]
Pfam	PF00456. Transketolase_N. 2 hits. [Graphical view]
PIRSF	PIRSF000156. Pyruvate_dh_E1. 1 hit.
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00759. aceE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODP1_BUCAI

Accession

Primary (citable) accession number: P57301

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program