Reviewed, UniProtKB/Swiss-Prot P57301 (ODP1_BUCAI)

Names and origin

Protein names

Recommended name:
    Pyruvate dehydrogenase E1 component
    EC=1.2.4.1

Gene names

Name:	aceE
Ordered Locus Names:	BU205

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (Acyrthosiphon pisum symbiotic bacterium) [Complete proteome] [HAMAP]

Taxonomic identifier

118099 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	887 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) By similarity.
Catalytic activity	Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO(2).
Cofactor	Thiamine pyrophosphate By similarity.
Subunit structure	Homodimer By similarity.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Pyruvate Thiamine pyrophosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	pyruvate dehydrogenase (acetyl-transferring) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 887	887	Pyruvate dehydrogenase E1 component		PRO_0000162240

Sequences

	Sequence		Length	Mass (Da)
	P57301-1 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: B6AB82C012826105	FASTA	887	101,396
	10 20 30 40 50 60 MSENLYNDVD PIETRDWVQA IESVIRREGH KRAHFLIEQV LKTAKINRKE FFRSSFTSDY 70 80 90 100 110 120 INTISREDEY EYPGNLILEK RIRSAIRWNA IMMVLRASKK NLELGGHLSS FQSSATIYEV 130 140 150 160 170 180 CFNHFFQAKN HKDGGDLVYF QGHISPGIYA RSFLEGRLSE EQIDNFRQEV DGIGLSSYPH 190 200 210 220 230 240 PKLMPNFWQF PTVSMGLGPL CAIYQAKFLK YLHNRELKNT SKQIVYAFLG DGEMDEPESK 250 260 270 280 290 300 GAISIAVREK LDNLIFIINC NLQRLDGPVV GNGKIVNELE SFFYGAGWKV IKVIWGSRWD 310 320 330 340 350 360 CLLKKDTSGK LIQLMNETVD GDYQTFKSKD GAYVRKYFFG KYKETYDLVK DMTDEEIWKL 370 380 390 400 410 420 NRGGHDPKKM FNALKKAKET KYKPTVILAH TVKGYGMGVI AEGKNIAHQI KKININGIIH 430 440 450 460 470 480 IRDRFNIPVS NDEINKLPYV TFKKNSEEYC YIHSQRKKLG GYIPFRLSSF TGKLILPKLI 490 500 510 520 530 540 DFQSLLEEQK KDISTTVAFI RVLNIILKNN SIKHLIVPII ADEARTFGME GLFRKIGIYS 550 560 570 580 590 600 SSGQKYTPQD REQLAYYKEE KKGQILQEGI NELGAASSWL AAATSYSTND FPMILFYIYY 610 620 630 640 650 660 SIFGFQRIGD LFWAAGDQQA RGFLIGGTSG RTTLNGEGLQ HEDGHSHIQS LTIPNCISYD 670 680 690 700 710 720 PAFAYEVAVI IQDGLRRMYG PSQENIYYYI TTINENYYMP AMPIGVEEGI CKGIYKLKTL 730 740 750 760 770 780 HGTTSKVQLI GSGAILRSVC EAAEILLKDY SITTDIYSVT SFTELARNGE DCERWNMLHP 790 800 810 820 830 840 NEKNKIAYVK QIMNKNPTVA ATDYMKLFAE QIRHYIPSQE YHVLGTDGFG RSDSRDKLRD 850 860 870 880 HFEVNAYYIV IAALNLLANI NDIKKKVVED AIMKFNIDAN KINPRLS

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tokyo 1998.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000003 Genomic DNA. Translation: BAB12922.1.
RefSeq	NP_240036.1.
3D structure databases
HSSP	HSSP built from PDB template 1L8A based on UniProtKB P06958.
SMR	P57301. Positions 58-887.
ModBase	Search...
Genome annotation databases
GeneID	1109648.
GenomeReviews	Gene locus BU205 in contig BA000003_GR.
KEGG	buc:BU205.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P57301.
Enzyme and pathway databases
BioCyc	BSP107806:BU205-MON.
Family and domain databases
InterPro	IPR004660. 2-oxoA_DHase_E1. IPR005474. Transketo_N. IPR015941. Transketolase_C-like. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF00456. Transketolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000156. Pyruvate_dh_E1. 1 hit.
TIGRFAMs	TIGR00759. aceE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ODP1_BUCAI

Accession

Primary (citable) accession number: P57301

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	November 4, 2008
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)