Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Penicillin-binding protein 1B

Gene

mrcB

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal domain (cross-linking of the peptide subunits) (By similarity).By similarity

Catalytic activityi

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate.

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei174 – 1741Proton donor; for transglycosylase activityBy similarity
Active sitei451 – 4511Acyl-ester intermediate; for transpeptidase activityBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Hydrolase, Transferase

Keywords - Biological processi

Antibiotic resistance, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Enzyme and pathway databases

BioCyciBAPH107806:GBZJ-199-MONOMER.
UniPathwayiUPA00219.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Penicillin-binding protein 1B
Short name:
PBP-1b
Short name:
PBP1b
Alternative name(s):
Murein polymerase
Including the following 2 domains:
Penicillin-insensitive transglycosylase (EC:2.4.1.129)
Alternative name(s):
Peptidoglycan TGase
Peptidoglycan glycosyltransferase
Penicillin-sensitive transpeptidase (EC:3.4.-.-)
Alternative name(s):
DD-transpeptidase
Gene namesi
Name:mrcB
Ordered Locus Names:BU200
OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Taxonomic identifieri107806 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
Proteomesi
  • UP000001806 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence analysis
Transmembranei9 – 2921Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini30 – 760731ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 760760Penicillin-binding protein 1BPRO_0000083185Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi107806.BU200.

Structurei

3D structure databases

ProteinModelPortaliP57296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni136 – 308173TransglycosylaseAdd
BLAST
Regioni392 – 684293TranspeptidaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the glycosyltransferase 51 family.Curated
In the C-terminal section; belongs to the transpeptidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000282711.
KOiK05365.
OMAiKDGYRYP.
OrthoDBiEOG6HMXCD.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.

Sequencei

Sequence statusi: Complete.

P57296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFFNFKKYFL IKVFFFVLIL TLCYGLYLYV KINRFINGKV WNFPTSIYGR
60 70 80 90 100
IVNLEPGNSY SQKEVLHLLK STMYRKVDLV MLPGEYSIKN NTIEFIRRAF
110 120 130 140 150
DFPDIREDEF HARLYFNKDT LVKIKNIDNN HDFSFFRLEP KLIAMLKSPE
160 170 180 190 200
AKKRMFIPRN QYPEMLVKTL LAIEDKYFYE HDGIHLSSIG RAFLVNLMAG
210 220 230 240 250
RTIQGGSTLT QQLIKNLFLT NTRSILRKIN EIYMALILDR FYTKDRILEL
260 270 280 290 300
YLNEVYLGQD GDEQIRGFPL ASIYYFGRPI NELNLEQYAL LVGMVKGASL
310 320 330 340 350
YSPWTNPNLA LKRRNLVLFL LYKQKYITRK IYKDLCKRSL NVQPKGNIIS
360 370 380 390 400
SHPSFIQLVC EEFHKKIYNP IKNFPGTKIF TTLDYTSQNA VEQAVKIEIP
410 420 430 440 450
ILKRKKRLKD LEVAMIVIDR FTGEVQALIG SSKPEFNGYN RALKTRRSIG
460 470 480 490 500
SLSKPITYLT ALSQPEKYHL NTWISNYPLS IKLDSGQYWT PKNNNFSFSK
510 520 530 540 550
KVLLLDALIH SINIPTVNLS INIGLKKLVD SWLLLGISKK YITPLPSISL
560 570 580 590 600
GAINLTPFEI AQVFQIIGSG GYKSSLSSVR SIISDDGKVL YQNLPQSIHI
610 620 630 640 650
ESSEASYLTL YGMQQVVKSG TAKSLGTIFK EFSLAGKTGT TNNLVDNWFV
660 670 680 690 700
GIDGKQIVIT WIGRDNNHTT RLYSSSGAMQ IYKRYLQYQR PVPLVLKAPN
710 720 730 740 750
NINMFYINNL GELFCKKNNQ HNRMLPIWSI KNKKICNDKL SERFSIKKKK
760
NFLFWLKNLF
Length:760
Mass (Da):88,003
Last modified:December 1, 2000 - v1
Checksum:i00B2C5B51F6947D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12917.1.
RefSeqiNP_240031.1. NC_002528.1.
WP_010895998.1. NC_002528.1.

Genome annotation databases

EnsemblBacteriaiBAB12917; BAB12917; BAB12917.
GeneIDi1109643.
KEGGibuc:BU200.
PATRICi21243918. VBIBucAph127364_0211.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000003 Genomic DNA. Translation: BAB12917.1.
RefSeqiNP_240031.1. NC_002528.1.
WP_010895998.1. NC_002528.1.

3D structure databases

ProteinModelPortaliP57296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi107806.BU200.

Protein family/group databases

CAZyiGT51. Glycosyltransferase Family 51.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAB12917; BAB12917; BAB12917.
GeneIDi1109643.
KEGGibuc:BU200.
PATRICi21243918. VBIBucAph127364_0211.

Phylogenomic databases

eggNOGiENOG4105BZ4. Bacteria.
COG0744. LUCA.
HOGENOMiHOG000282711.
KOiK05365.
OMAiKDGYRYP.
OrthoDBiEOG6HMXCD.

Enzyme and pathway databases

UniPathwayiUPA00219.
BioCyciBAPH107806:GBZJ-199-MONOMER.

Family and domain databases

Gene3Di3.40.710.10. 1 hit.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR001264. Glyco_trans_51.
IPR023346. Lysozyme-like_dom.
IPR011813. PBP_1b.
IPR001460. PCN-bd_Tpept.
IPR028166. UB2H.
[Graphical view]
PfamiPF00912. Transgly. 1 hit.
PF00905. Transpeptidase. 1 hit.
PF14814. UB2H. 1 hit.
[Graphical view]
PIRSFiPIRSF002799. PBP_1b. 1 hit.
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF56601. SSF56601. 2 hits.
TIGRFAMsiTIGR02071. PBP_1b. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
    Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
    Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: APS.

Entry informationi

Entry nameiPBPB_BUCAI
AccessioniPrimary (citable) accession number: P57296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: November 11, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
    Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.