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P57295

- TRUA_BUCAI

UniProt

P57295 - TRUA_BUCAI

Protein

tRNA pseudouridine synthase A

Gene

truA

Organism
Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 1 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.UniRule annotation

    Catalytic activityi

    tRNA uridine(38-40) = tRNA pseudouridine(38-40).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei57 – 571NucleophileUniRule annotation
    Binding sitei115 – 1151SubstrateUniRule annotation

    GO - Molecular functioni

    1. pseudouridine synthase activity Source: UniProtKB-HAMAP
    2. RNA binding Source: InterPro

    GO - Biological processi

    1. tRNA pseudouridine synthesis Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    tRNA processing

    Enzyme and pathway databases

    BioCyciBAPH107806:GBZJ-198-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA pseudouridine synthase AUniRule annotation (EC:5.4.99.12UniRule annotation)
    Alternative name(s):
    tRNA pseudouridine(38-40) synthaseUniRule annotation
    tRNA pseudouridylate synthase IUniRule annotation
    tRNA-uridine isomerase IUniRule annotation
    Gene namesi
    Name:truAUniRule annotation
    Ordered Locus Names:BU199
    OrganismiBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)
    Taxonomic identifieri107806 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
    ProteomesiUP000001806: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 266266tRNA pseudouridine synthase APRO_0000057348Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliP57295.
    SMRiP57295. Positions 6-264.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tRNA pseudouridine synthase TruA family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0101.
    HOGENOMiHOG000248672.
    KOiK06173.
    OMAiQANAFVH.
    OrthoDBiEOG6Z9B4R.

    Family and domain databases

    Gene3Di3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    HAMAPiMF_00171. TruA.
    InterProiIPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view]
    PANTHERiPTHR11142. PTHR11142. 1 hit.
    PfamiPF01416. PseudoU_synth_1. 2 hits.
    [Graphical view]
    PIRSFiPIRSF001430. tRNA_psdUrid_synth. 1 hit.
    SUPFAMiSSF55120. SSF55120. 1 hit.
    TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P57295-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVAKNVKTFA LGVEYDGSYY HGWQRQKILP SIQEEIEKAL SIIANHKIDV    50
    VCAGRTDAGV HSIGQVIHFK TTANRKKSSW SIGVNSYLSE NISVVWVKEV 100
    TENFHARYSA ITRSYRYIIY NYSLRSAIFQ TKLNHIYRKL NVDKMNFEAQ 150
    FLLGEHDFTS FRALGCQSHS PWRNITKLNV FRFHNWVVVD ITANSFLHHM 200
    VRNIVGSLIE VGISKKKEYW IKDLLEKKDR SHAGATAPAK GLYLVYVEYP 250
    LHFNLPRSAY TSIFFK 266
    Length:266
    Mass (Da):30,769
    Last modified:December 1, 2000 - v1
    Checksum:i112C5F3322459ACD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12916.1.
    RefSeqiNP_240030.1. NC_002528.1.
    WP_010895997.1. NC_002528.1.

    Genome annotation databases

    EnsemblBacteriaiBAB12916; BAB12916; BAB12916.
    GeneIDi1109642.
    KEGGibuc:BU199.
    PATRICi21243916. VBIBucAph127364_0210.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000003 Genomic DNA. Translation: BAB12916.1 .
    RefSeqi NP_240030.1. NC_002528.1.
    WP_010895997.1. NC_002528.1.

    3D structure databases

    ProteinModelPortali P57295.
    SMRi P57295. Positions 6-264.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB12916 ; BAB12916 ; BAB12916 .
    GeneIDi 1109642.
    KEGGi buc:BU199.
    PATRICi 21243916. VBIBucAph127364_0210.

    Phylogenomic databases

    eggNOGi COG0101.
    HOGENOMi HOG000248672.
    KOi K06173.
    OMAi QANAFVH.
    OrthoDBi EOG6Z9B4R.

    Enzyme and pathway databases

    BioCyci BAPH107806:GBZJ-198-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.580. 1 hit.
    3.30.70.660. 1 hit.
    HAMAPi MF_00171. TruA.
    InterProi IPR020103. PsdUridine_synth_cat_dom.
    IPR001406. PsdUridine_synth_TruA.
    IPR020097. PsdUridine_synth_TruA_a/b_dom.
    IPR020095. PsdUridine_synth_TruA_C.
    IPR020094. PsdUridine_synth_TruA_N.
    [Graphical view ]
    PANTHERi PTHR11142. PTHR11142. 1 hit.
    Pfami PF01416. PseudoU_synth_1. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF001430. tRNA_psdUrid_synth. 1 hit.
    SUPFAMi SSF55120. SSF55120. 1 hit.
    TIGRFAMsi TIGR00071. hisT_truA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
      Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
      Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: APS.

    Entry informationi

    Entry nameiTRUA_BUCAI
    AccessioniPrimary (citable) accession number: P57295
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 79 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Buchnera aphidicola (subsp. Acyrthosiphon pisum)
      Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3