P57295 (TRUA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine synthase A
EC=5.4.99.12

Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I

Gene names

Name:	truA
Ordered Locus Names:	BU199

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Acyrthosiphon pisum)

Protein attributes

Sequence length	266 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171
Catalytic activity	tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Isomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	tRNA pseudouridine synthesis Inferred from electronic annotation. Source: HAMAP
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 266	266	tRNA pseudouridine synthase A HAMAP-Rule MF_00171		PRO_0000057348
Sites
	Active site	57	1	Nucleophile By similarity
	Binding site	115	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57295 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 112C5F3322459ACD	FASTA	266	30,769
	10 20 30 40 50 60 MVAKNVKTFA LGVEYDGSYY HGWQRQKILP SIQEEIEKAL SIIANHKIDV VCAGRTDAGV 70 80 90 100 110 120 HSIGQVIHFK TTANRKKSSW SIGVNSYLSE NISVVWVKEV TENFHARYSA ITRSYRYIIY 130 140 150 160 170 180 NYSLRSAIFQ TKLNHIYRKL NVDKMNFEAQ FLLGEHDFTS FRALGCQSHS PWRNITKLNV 190 200 210 220 230 240 FRFHNWVVVD ITANSFLHHM VRNIVGSLIE VGISKKKEYW IKDLLEKKDR SHAGATAPAK 250 260 GLYLVYVEYP LHFNLPRSAY TSIFFK

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12916.1.
RefSeq	NP_240030.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57295.
SMR	P57295. Positions 6-264.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB12916; BAB12916; BAB12916.
GeneID	1109642.
KEGG	buc:BU199.
PATRIC	21243916. VBIBucAph127364_0210.
Phylogenomic databases
eggNOG	COG0101.
HOGENOM	HOG000248672.
KO	K06173.
OMA	TPFRNVH.
ProtClustDB	PRK00021.
Family and domain databases
Gene3D	3.30.70.580. 1 hit. 3.30.70.660. 1 hit.
HAMAP	MF_00171. TruA.
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
PANTHER	PTHR11142. PTHR11142. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
PIRSF	PIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. hisT_truA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRUA_BUCAI

Accession

Primary (citable) accession number: P57295

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program