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P57295 (TRUA_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA pseudouridine synthase A

EC=5.4.99.12
Alternative name(s):
tRNA pseudouridine(38-40) synthase
tRNA pseudouridylate synthase I
tRNA-uridine isomerase I
Gene names
Name:truA
Ordered Locus Names:BU199
OrganismBuchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium) [Reference proteome] [HAMAP]
Taxonomic identifier107806 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs By similarity. HAMAP-Rule MF_00171

Catalytic activity

tRNA uridine(38-40) = tRNA pseudouridine(38-40). HAMAP-Rule MF_00171

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00171

Sequence similarities

Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
   Biological processtRNA processing
   Molecular functionIsomerase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processtRNA pseudouridine synthesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: InterPro

pseudouridine synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266tRNA pseudouridine synthase A HAMAP-Rule MF_00171
PRO_0000057348

Sites

Active site571Nucleophile By similarity
Binding site1151Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P57295 [UniParc].

Last modified December 1, 2000. Version 1.
Checksum: 112C5F3322459ACD

FASTA26630,769
        10         20         30         40         50         60 
MVAKNVKTFA LGVEYDGSYY HGWQRQKILP SIQEEIEKAL SIIANHKIDV VCAGRTDAGV 

        70         80         90        100        110        120 
HSIGQVIHFK TTANRKKSSW SIGVNSYLSE NISVVWVKEV TENFHARYSA ITRSYRYIIY 

       130        140        150        160        170        180 
NYSLRSAIFQ TKLNHIYRKL NVDKMNFEAQ FLLGEHDFTS FRALGCQSHS PWRNITKLNV 

       190        200        210        220        230        240 
FRFHNWVVVD ITANSFLHHM VRNIVGSLIE VGISKKKEYW IKDLLEKKDR SHAGATAPAK 

       250        260 
GLYLVYVEYP LHFNLPRSAY TSIFFK 

« Hide

References

[1]"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS."
Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.
Nature 407:81-86(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: APS.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000003 Genomic DNA. Translation: BAB12916.1.
RefSeqNP_240030.1. NC_002528.1.

3D structure databases

ProteinModelPortalP57295.
SMRP57295. Positions 6-264.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB12916; BAB12916; BAB12916.
GeneID1109642.
KEGGbuc:BU199.
PATRIC21243916. VBIBucAph127364_0210.

Phylogenomic databases

eggNOGCOG0101.
HOGENOMHOG000248672.
KOK06173.
OMAQANAFVH.
OrthoDBEOG6Z9B4R.
ProtClustDBPRK00021.

Enzyme and pathway databases

BioCycBAPH107806:GBZJ-198-MONOMER.

Family and domain databases

Gene3D3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPMF_00171. TruA.
InterProIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERPTHR11142. PTHR11142. 1 hit.
PfamPF01416. PseudoU_synth_1. 2 hits.
[Graphical view]
PIRSFPIRSF001430. tRNA_psdUrid_synth. 1 hit.
SUPFAMSSF55120. SSF55120. 1 hit.
TIGRFAMsTIGR00071. hisT_truA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRUA_BUCAI
AccessionPrimary (citable) accession number: P57295
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: February 19, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Buchnera aphidicola (subsp. Acyrthosiphon pisum)

Buchnera aphidicola (subsp. Acyrthosiphon pisum): entries and gene names