P57292 (PANC_BUCAI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate synthetase
Short name=PS
EC=6.3.2.1

Alternative name(s):
Pantoate--beta-alanine ligase
Pantoate-activating enzyme

Gene names

Name:	panC
Ordered Locus Names:	BU196

Organism

Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon pisum symbiotic bacterium)

Taxonomic identifier

107806 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	285 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate By similarity. HAMAP MF_00158
Catalytic activity	ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate. HAMAP MF_00158
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1. HAMAP MF_00158
Subunit structure	Homodimer By similarity. HAMAP MF_00158
Subcellular location	Cytoplasm Potential HAMAP MF_00158.
Miscellaneous	The reaction proceeds by a bi uni uni bi ping pong mechanism By similarity. HAMAP MF_00158
Sequence similarities	Belongs to the pantothenate synthetase family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW pantoate-beta-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 285	285	Pantothenate synthetase HAMAP MF_00158		PRO_0000128212
Regions
	Nucleotide binding	30 – 37	8	ATP By similarity
	Nucleotide binding	149 – 152	4	ATP By similarity
	Nucleotide binding	186 – 189	4	ATP By similarity
Sites
	Active site	37	1	Proton donor By similarity
	Binding site	61	1	Beta-alanine By similarity
	Binding site	61	1	Pantoate By similarity
	Binding site	155	1	Pantoate By similarity
	Binding site	178	1	ATP; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P57292 [UniParc]. Last modified December 1, 2000. Version 1. Checksum: 655446232252F0F9	FASTA	285	33,269
	10 20 30 40 50 60 MHIIKTIKVL YKEIKILKKS NKKIGLVPTM GNLHDGHIKL ILLAKKYSDI IIVSIFINPM 70 80 90 100 110 120 QFDNLSDLKN YPKTFMKDSI ILKKYHVDIL FFPHINEIYP NGIEHQTFVE VIKLSKILEG 130 140 150 160 170 180 QSRPGHFRGV TTIITKLFNF IQPDFAFFGE KDYQQLLIIK ILVKELNYMI KIISLPTIRL 190 200 210 220 230 240 KNGLALSSRN NYLSSQENEI APYLYKIIKK TCEKIIKEDD NIRPKIIHES KILLIKKGFS 250 260 270 280 VDIFDIYDYK NLEHPSKKVK KVILLASVWL GNTRLIDNKK IILNY

References

[1]	"Genome sequence of the endocellular bacterial symbiont of aphids Buchnera sp. APS." Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H. Nature 407:81-86(2000) [PubMed: 10993077] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: APS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000003 Genomic DNA. Translation: BAB12913.1.
RefSeq	NP_240027.1. NC_002528.1.
3D structure databases
ProteinModelPortal	P57292.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000002870; EBBUCP00000002646; EBBUCG00000002870.
GeneID	1109639.
GenomeReviews	Gene locus BU196 in contig BA000003_GR.
KEGG	buc:BU196.
PATRIC	21243910. VBIBucAph127364_0207.
Phylogenomic databases
GeneTree	EBGT00050000007772.
HOGENOM	HBG428839.
OMA	EMDGLAM.
PhylomeDB	P57292.
ProtClustDB	PRK00380.
Enzyme and pathway databases
BioCyc	BSP107806:BU196-MONOMER.
Family and domain databases
HAMAP	MF_00158. PanC. [Tree]
InterPro	IPR003721. Pantoate_ligase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01918.
PANTHER	PTHR21299:SF1. Pantoate_ligase. 1 hit.
Pfam	PF02569. Pantoate_ligase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00018. PanC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANC_BUCAI

Accession

Primary (citable) accession number: P57292

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program